Quantitative proteomic analysis of Ibuprofen-degrading Patulibacter sp. strain I11

The constant increase in diversity and quantity of Pharmaceutically Active Compounds (PhACs) detected in waste water treatment plants (WWTPs) effluents is an issue of rising concern due to the potential negative impact of the PhACs on the surrounding environment1. Of PhACs, Ibuprofen, a non-steroidal anti-inflammatory drug, is considered one of the most severe contaminants in the influent of WWTPs, typically being found in the range of 10-400 μg/L. Concentrations of Ibuprofen in the effluent wastewater is considerably lower indicating a partial removal of Ibuprofen2,3. This elimination of ibuprofen is being ascribed to primarily to biodegradation. However, if biodegradation is to be used as a solution for removing ibuprofen from wastewater, figuring out which bacteria are responsible for degrading the compound and understanding the processes by which it is being degraded are the first steps on the way. In the current study, the pathway of degradation of ibuprofen of the ibuprofen degrading strain Patulibacter sp. strain I11 was characterised using quantitative proteomics. The subsequent data analysis revealed several proteins which were up-regulated in response to ibuprofen by Patulibacter sp. strain I11 that might be involved in the degradation of ibuprofen.<br/

A Conformally Invariant Holographic Two-Point Function on the Berger Sphere

We apply our previous work on Green's functions for the four-dimensional quaternionic Taub-NUT manifold to obtain a scalar two-point function on the homogeneously squashed three-sphere (otherwise known as the Berger sphere), which lies at its conformal infinity. Using basic notions from conformal geometry and the theory of boundary value problems, in particular the Dirichlet-to-Robin operator, we establish that our two-point correlation function is conformally invariant and corresponds to a boundary operator of conformal dimension one. It is plausible that the methods we use could have more general applications in an AdS/CFT context.Comment: 1+49 pages, no figures. v2: Several typos correcte

Analysis of norfloxacin ecotoxicity and the relation with its degradation by means of electrochemical oxidation using different anodes

[EN] In this work, ecotoxicological bioassays based on Lactuca sativa seeds and bioluminescent bacterium (Vibrio fischeri) have been carried out in order to quantify the toxicity of Norfloxacin (NOR) and sodium sulfate solutions, before and after treating them using electrochemical advanced oxidation. The effect of some process variables (anode material, reactor configuration and applied current) on the toxicity evolution of the treated solution has been studied. A NOR solution shows an EC50 (5 days) of 336 mg L-1 towards Lactuca sativa. This threshold NOR concentration decreases with sodium sulfate concentration, in solutions that contain simultaneously Norfloxacin and sodium sulfate. In every case considered in this work, the electrochemical advanced oxidation process increased the toxicity (towards both Lactuca sativa and Vibrio fischeri) of the solution. This toxicity increase is mainly due to the persulfate formation during the electrochemical treatment. From a final solution toxicity point of view, the best results were obtained using a BDD anode in a divided reactor applying the lowest current intensity.The authors are very grateful to the Ministerio de Economia y Competitividad (Projects CTQ2015-65202-C2-1-R and RTI2018-101341-B-C21) for their economic support.Montañés, M.; García Gabaldón, M.; Roca-Pérez, L.; Giner-Sanz, JJ.; Mora-Gómez, J.; Pérez-Herranz, V. (2020). Analysis of norfloxacin ecotoxicity and the relation with its degradation by means of electrochemical oxidation using different anodes. Ecotoxicology and Environmental Safety. 188:1-10. https://doi.org/10.1016/j.ecoenv.2019.109923S110188Banks, M. K., & Schultz, K. E. (2005). Comparison of Plants for Germination Toxicity Tests in Petroleum-Contaminated Soils. Water, Air, and Soil Pollution, 167(1-4), 211-219. doi:10.1007/s11270-005-8553-4Barreto, J. P. d. P., Araujo, K. C. d. F., de Araujo, D. M., & Martinez-Huitle, C. A. (2015). 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Toxics, 6(1), 8. doi:10.3390/toxics6010008Mora-Gómez, J., García-Gabaldón, M., Ortega, E., Sánchez-Rivera, M.-J., Mestre, S., & Pérez-Herranz, V. (2018). Evaluation of new ceramic electrodes based on Sb-doped SnO2 for the removal of emerging compounds present in wastewater. Ceramics International, 44(2), 2216-2222. doi:10.1016/j.ceramint.2017.10.178Mora-Gomez, J., Ortega, E., Mestre, S., Pérez-Herranz, V., & García-Gabaldón, M. (2019). Electrochemical degradation of norfloxacin using BDD and new Sb-doped SnO2 ceramic anodes in an electrochemical reactor in the presence and absence of a cation-exchange membrane. Separation and Purification Technology, 208, 68-75. doi:10.1016/j.seppur.2018.05.017Özcan, A., Atılır Özcan, A., & Demirci, Y. (2016). Evaluation of mineralization kinetics and pathway of norfloxacin removal from water by electro-Fenton treatment. Chemical Engineering Journal, 304, 518-526. doi:10.1016/j.cej.2016.06.105Priac, A., Badot, P.-M., & Crini, G. (2017). 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Review of new physics effects in t-tbar production

Both CDF and DO report a forward-backward asymmetry in t-tbar production that is above the standard model prediction. We review new physics models that can give a large forward backward asymmetry in t-tbar production at the Tevatron and the constraints these models face from searches for dijet resonances and contact interactions, from flavor physics and the t-tbar cross section. Expected signals at the LHC are also reviewed.Comment: 18 pages, 18 figures, 4 tables, invited review for a special "Top and flavour physics in the LHC era" issue of The European Physical Journal C, we invite comments regarding contents of the revie