Associations between Wolbachia, maize and Diabrotica virgifera virgifera

Title from PDF of title page (University of Missouri--Columbia, viewed on Feb. 24, 2010).The entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract appears in the public.pdf file.Dissertation advisor: Dr. Georgia Davis.VitaPh. D. University of Missouri--Columbia 2008.Diabrotica virgifera virgifera which is infected with Wolbachia, is the most significant and widespread pest to maize in North America and Europe. Wolbachia are obligate intracellular bacteria which seem to be limited to ecdyzoan hosts. Many Wolbachia hosts induce or vector serious human diseases resulting in the loss of millions of lives annually. The majority of differentially expressed genes identified in a microarray experiment performed to locate endogenous sources of D. virgifera virgifera resistance in maize, Zea mays L., are normally involved in microbial defense rather than wounding or insect attack. A second microarray experiment to determine whether Wolbachia were influencing the response of maize to D. virgifera virgifera attack indicated the presence of Wolbachia in the insect can down regulate genes in all plant defense classes. This may contribute to the success of D. virgifera virgifera as a maize pest. To further test this idea, assays on D. virgifera virgifera larval competitiveness and fertility were performed. Results of the assay suggest D. virgifera virgifera and Wolbachia share a commensal association.Includes bibliographical reference

Achieving escape velocity: Breaking Free from the impact failure of applied philosophy

As in the sciences, the humanities also feel the pressure to demonstrate societal relevance. Applied philosophy is a natural place to look. But how has it fared in terms of having an impact? Adam Briggle, Robert Frodeman, and Kelli Barr are investigating the impact of philosophical work on both the STEM disciplines and society. Historically, philosophers have not been particularly self-conscious about how their insights are being taken up outside the academy. They argue the problem is not what they say, but to whom they are speaking and where

Nicholas and Anna Ricco Ethics Awards

This paper was awarded a Nicholas and Anna Ricco Ethics Award for 2013. In this paper, the author discusses issues related to accountability versus autonomy and suggestions toward a more responsible practice of science

Practicing Relevance: The Origins, Practices, and Future of Applied Philosophy

This dissertation takes up the question of the social function of philosophy. Popular accounts of the nature and value of philosophy reinforce long-standing perceptions that philosophy is useless or irrelevant to pressing societal problems. Yet, the increasingly neoliberal political-economic environment of higher education places a premium on mechanisms that link public funding for research to demonstrations of return on investment in the form of benefitting broader society. This institutional situation presents a philosophical problem warranting professional attention. This project offers a diagnosis of the problem and develops a way forward from it. Drawing from Foucauldian archaeological methods, my analysis focuses on the interplay of institutional structures and intellectual practices. Since the early 20th century, departments of philosophy on college and university campuses have been the center of gravity for professional philosophy in the US. Establishing this institutional ‘home' for philosophers drove the adoption of disciplinary practices, norms, and standards for inquiry. But the metaphilosophical assumptions underpinning disciplinarity have become problematic, I argue: they are poor guides for navigating the situation of higher education in the 21st century. Several movements within the profession of philosophy during the 1960s and 70s sought to reverse philosophers' general retreat from public affairs. Applied philosophy, environmental philosophy, and bioethics each offer a case study in attempts to address the problem of societal relevance. However, surveys of the journal literature in each field uncovered few reflections on whether or not individual projects, or the field as a whole, had any impact on the societal problems to which those philosophers turned their attention. This suggests a need for further thinking about implementation – how to institutionalize alternative practices of philosophy that do demonstrate societal relevance. By way of conclusion, I offer some of the necessary groundwork toward a philosophy of implementation, in the form of discussing significant questions and challenges confronting philosophers who aim for societal relevance both in principle and in practice

Re-engineering Ethics: Pushing Philosophy Outside of its Comfort Zone at the APPE Annual Meeting

This article discusses ethics and pushing philosophy outside of its comfort zone at the Association for Practical and Professional Ethics (APPE) annual meeting

Chikungunya outbreak in Karachi Pakistan 2016-2017: An analysis of viral isolates

In December 2016 physicians in Karachi, Pakistan,witnessed an increase in patients presenting with febrile illness and severe polyarthralgia. Subsequently, chikungunya virus (CHIKV)) was isolated from three patients. This virus was sequenced and compared with other isolates of CHIKV obtained in India and Pakistan during recent outbreaks. Phylogenetic analysis indicated that the Karachi isolates were most similar to the East Central South African CHIKV lineage and showed sequence homology to isolates obtained in other parts of Pakistan and India. More importantly, two of the CHIKV isolates had a nucleotide substitution in the E1 gene corresponding to an amino acid change at chain F portion of the E1 protein

Research Impact: We need negative metrics too

Article discussing different ways to indicate the impact of a researcher's scholarly activities

Evidence of chikungunya virus disease in Pakistan since 2015 with patients demonstrating involvement of the central nervous system

Several arboviruses are endemic to and co-circulate in Pakistan. In recent years, Pakistan has observed a rise in arboviral infections. A cross-sectional study for arboviral diseases, which included screening for Chikungunya virus (CHIKV), was initiated in 2015 to determine which pathogens were causing disease in patients presenting to health care services. Exposure to CHIKV was verified via detection of viral nucleic acids or virus-specific IgM with virus-specific neutralizing antibodies. Out of 997 enrolled patients presenting with clinical features suggestive of arboviral disease, 102 patients were positive for CHIKV IgM antibodies and 60 patients were positive for CHIKV nucleic acids or neutralizing antibodies. The data presented here show that CHIKV has been circulating in Pakistan since April of 2015. CHIKV infections were detected in study subjects up to the conclusion of our enrollment period in July 2017. Syndromic and clinical data show that arthralgia was associated with CHIKV as was rash, fever greater than 38°C, and lymphopenia. Neurological symptoms were reported in 49% of CHIKV suspect patients and in 46.6% of confirmed infections. Acute disseminated encephalomyelitis was diagnosed in 5% of confirmed infection and various manifestation of encephalitis diagnosed in an additional 16.6% of patients with confirmed CHIKV infections. CHIKV-exposed patients were just as likely to present with neurological symptoms and encephalitis as patients with West Nile Virus infections but were 4.57 times more likely to have lymphopenia. This proportion of neurological symptoms may be a complicating factor in countries where WNV and/or JEV co-circulate with CHIKV

Comparison of clinical presentation and out-comes of chikungunya and dengue virus infections in patients with acute undifferentiated febrile illness from the Sindh region of Pakistan

Background: Arboviruses are a cause of acute febrile illness and outbreaks worldwide. Recent outbreaks of Chikungunya virus (CHIKV) in dengue endemic areas have alarmed clinicians as unique clinical features differentiating CHIKV from Dengue virus (DENV) are limited. This has complicated diagnostic efforts especially in resource limited countries where lab testing is not easily available. Therefore, it is essential to analyse and compare clinical features of laboratory confirmed cases to assist clinicians in suspecting possible CHIKV infection at time of clinical presentation.Methodology: A prospective point prevalence study was conducted, with the hypothesis that not all patients presenting with clinical suspicion of dengue infections at local hospitals are suffering from dengue and that other arboviruses such as Chikungunya, West Nile viruses, Japanese Encephalitis virus and Zika virus are co-circulating in the Sindh region of Pakistan. Out-patients and hospitalized (in-patients) of selected district hospitals in different parts of Sindh province of Pakistan were recruited. Patients with presumptive dengue like illness (Syndromic diagnosis) by the treating physicians were enrolled between 2015 and 2017. Current study is a subset of larger study mentioned above. Here-in we compared laboratory confirmed cases of CHIKV and DENV to assess clinical features and laboratory findings that may help differentiate CHIKV from DENV infection at the time of clinical presentation.Results: Ninety-eight (n = 98) cases tested positive for CHIKV, by IgM and PCR and these were selected for comparative analysis with DENV confirmed cases (n = 171). On multivariable analysis, presence of musculoskeletal [OR = 2.5 (95% CI:1.6-4.0)] and neurological symptoms [OR = 4.4 (95% CI:1.9-10.2)], and thrombocytosis [OR = 2.2 (95% CI:1.1-4.0)] were associated with CHIKV infection, while atypical lymphocytes [OR = 8.3 (95% CI:4.2-16.7)] and thrombocytopenia [OR = 8.1 (95% CI:1.7-38.8)] were associated with DENV cases at time of presentation. These findings may help clinicians in differentiating CHIKV from DENV infection.Conclusion: CHIKV is an important cause of illness amongst patients presenting with acute febrile illness in Sindh region of Pakistan. Arthralgia and encephalitis at time of presentation among patients with dengue-like illness should prompt suspicion of CHIKV infection, and laboratory confirmation must be sought

Human west nile virus disease outbreak in Pakistan, 2015–2016

Like most of the world, Pakistan has seen an increase in mosquito-transmitted diseases in recent years. The magnitude and distribution of these diseases are poorly understood as Pakistan does not have a nation-wide system for reporting disease. A cross-sectional study to determine which flaviviruses were causing of arboviral disease in Pakistan was instituted. West Nile virus (WNV) is a cause of seasonal fever with neurotropic findings in countries that share borders with Pakistan. Here, we describe the active and persistent circulation of WNV in humans in the southern region of Pakistan. This is the first report of WNV causing neurological disease in human patients in this country. Of 997 enrolled patients presenting with clinical features suggestive of arboviral disease, 105 were positive for WNV IgM antibodies, and 71 of these patients possessed WNV-specific neutralizing antibodies. Cross-reactivity of WNV IgM antibodies with Japanese encephalitis virus(JEV) occurred in 75 of these 105 patients. WNV co-infections with Dengue viruses were not a contributing factor for the severity of disease. Nor did prior exposure to dengue virus contribute to incidence of neurological involvement in WNV-infected patients. Patients with WNV infections were more likely to present with altered mental status, seizures, and reduced Glasgow Coma scores when compared with JEV-infected patients. Human WNV cases and vector numbers exhibited a temporal correlation with climate