6 research outputs found
ΠΠ·Π°ΠΈΠΌΠΎΡΠ²ΡΠ·Ρ ΠΏΠΎΠ»ΠΈΠΌΠΎΡΡΠ½ΡΡ Π»ΠΎΠΊΡΡΠΎΠ², ΡΠ°ΡΠΏΠΎΠ»ΠΎΠΆΠ΅Π½Π½ΡΡ Π² ΠΏΡΠΎΠΌΠΎΡΠΎΡΠ½ΡΡ ΠΎΠ±Π»Π°ΡΡΡΡ Π³Π΅Π½ΠΎΠ² VEGF (rs699947 ΠΈ rs2010963), ICAM1 (rs281437) ΠΈ ET-1 (rs1800541), Ρ ΡΡΠΎΠ²Π½Π΅ΠΌ ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²ΡΡΡΠΈΡ Π±Π΅Π»ΠΊΠΎΠ²ΡΡ ΠΏΡΠΎΠ΄ΡΠΊΡΠΎΠ² Π² ΡΡΠ²ΠΎΡΠΎΡΠΊΠ΅ ΠΊΡΠΎΠ²ΠΈ ΠΈ ΡΠΈΡΠΊΠΎΠΌ ΡΠ°Π·Π²ΠΈΡΠΈΡ Π°Π»ΠΊΠΎΠ³ΠΎΠ»ΡΠ½ΠΎΠ³ΠΎ ΡΠΈΡΡΠΎΠ·Π° ΠΏΠ΅ΡΠ΅Π½ΠΈ
Background: Uncontrolled use of alcohol can lead to the development of cirrhosis of the liver, which is manifested by fibrosis with the formation of regenerative nodes, an increase in pressure in the portal vein system and impaired liver function. Hepatic endothelium dysfunction during the formation of portal hypertension is accompanied by an increase in the level of protein molecules involved in the functioning of the endothelium: vascular endothelial growth factor A (VEGF-A), a soluble form of the intercellular adhesion molecule (s-ICAM-1) and endothelin-1 (ET -one). It is assumed that elevated levels of VEGF-A, s-ICAM-1 and ET-1 in alcoholic liver cirrhosis (AHC) may be interconnected with the structure of polymorphic loci, the promoter regions of the respective genes, which in turn may be a genetic risk factor for developing cirrhosis.Aims: Investigate the relationship of carriage of variant forms of polymorphic loci located in the promoter regions of VEGF-A, ICAM-1 and ET-1 with the level of the corresponding proteins in the blood serum and the risk of AHC.Materials and methods: The main group consisted of patients with pathological dependence on alcohol, aggravated by cirrhosis of the liver (AHC, n=60). The control group consisted of persons suffering from alcohol abuse, without liver pathology (AA, n=24). The observation period was the period of hospitalization. The serum levels of VEGF-A, s-ICAM-1 and ET-1 were evaluated by enzyme immunoassay. The distribution of variant forms of polymorphic loci located in the promoter regions of the VEGF-A genes (rs699947 and rs2010963), ICAM1 (rs281437) and ET-1 (rs1800541) in the studied sample was performed by real-time PCR.Results: The development of alcoholic cirrhosis was accompanied by a significant increase in the concentration of VEGF-A, s-ICAM-1 and ET-1 in serum. At the same time, direct correlations between the concentrations of VEGF-A, s-ICAM-1 and ET-1 in serum and the diameter of the portal vein in persons with liver cirrhosis were revealed. Patients with AHC are often carriers of the G allele of rs1800541 locus, located in the promoter of the ET-1 gene, compared with individuals suffering from control without liver pathology, which is associated with an increased risk of developing cirrhosis in alcohol dependence. The carriage of the C allele rs699947, as well as the C allele rs2010963 located in the promoter of the VEGF gene was associated with an increased level of VEGF-A in the AHC compared to carriers of this allele in the AA group. In addition, in the group of patients with AHC, carriers of allele C, homozygous CC genotype and heterozygous GC genotype of rs2010963 locus compared with carriers of G allele or homozygous GG genotype, respectively, were characterized by elevated serum VEGF-A levels.Conclusion: Carrier allele G of the rs1800541 locus (ET-1) is a risk factor for liver cirrhosis with alcohol abuse. The carriage of the C allele rs699947, as well as the C allele rs2010963 located in the promoter of the VEGF gene, can determine the elevated serum VEGF-A level in the AHC.ΠΠ±ΠΎΡΠ½ΠΎΠ²Π°Π½ΠΈΠ΅. ΠΠ΅ΠΊΠΎΠ½ΡΡΠΎΠ»ΠΈΡΡΠ΅ΠΌΠΎΠ΅ ΡΠΏΠΎΡΡΠ΅Π±Π»Π΅Π½ΠΈΠ΅ Π°Π»ΠΊΠΎΠ³ΠΎΠ»Ρ ΠΌΠΎΠΆΠ΅Ρ ΠΎΠ±ΡΡΠ»ΠΎΠ²Π»ΠΈΠ²Π°ΡΡ ΡΠ°Π·Π²ΠΈΡΠΈΠ΅ ΡΠΈΡΡΠΎΠ·Π° ΠΏΠ΅ΡΠ΅Π½ΠΈ, ΠΊΠΎΡΠΎΡΡΠΉ ΠΏΡΠΎΡΠ²Π»ΡΠ΅ΡΡΡ ΡΠΈΠ±ΡΠΎΠ·ΠΎΠΌ Ρ ΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΡΠ·Π»ΠΎΠ²-ΡΠ΅Π³Π΅Π½Π΅ΡΠ°ΡΠΎΠ², ΠΏΠΎΠ²ΡΡΠ΅Π½ΠΈΠ΅ΠΌ Π΄Π°Π²Π»Π΅Π½ΠΈΡ Π² ΡΠΈΡΡΠ΅ΠΌΠ΅ Π²ΠΎΡΠΎΡΠ½ΠΎΠΉ Π²Π΅Π½Ρ ΠΈ Π½Π°ΡΡΡΠ΅Π½ΠΈΠ΅ΠΌ ΡΡΠ½ΠΊΡΠΈΠΈ ΠΏΠ΅ΡΠ΅Π½ΠΈ. ΠΠΈΡΡΡΠ½ΠΊΡΠΈΡ ΠΏΠ΅ΡΠ΅Π½ΠΎΡΠ½ΠΎΠ³ΠΎ ΡΠ½Π΄ΠΎΡΠ΅Π»ΠΈΡ ΠΏΡΠΈ ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΠΈ ΠΏΠΎΡΡΠ°Π»ΡΠ½ΠΎΠΉ Π³ΠΈΠΏΠ΅ΡΡΠ΅Π½Π·ΠΈΠΈ ΡΠΎΠΏΡΠΎΠ²ΠΎΠΆΠ΄Π°Π΅ΡΡΡ ΠΏΠΎΠ²ΡΡΠ΅Π½ΠΈΠ΅ΠΌ ΡΡΠΎΠ²Π½Ρ ΠΌΠΎΠ»Π΅ΠΊΡΠ» Π±Π΅Π»ΠΊΠΎΠ²ΠΎΠΉ ΠΏΡΠΈΡΠΎΠ΄Ρ, ΡΡΠ°ΡΡΠ²ΡΡΡΠΈΡ
Π² ΡΡΠ½ΠΊΡΠΈΠΎΠ½ΠΈΡΠΎΠ²Π°Π½ΠΈΠΈ ΡΠ½Π΄ΠΎΡΠ΅Π»ΠΈΡ, β Π²Π°ΡΠΊΡΠ»ΠΎΡΠ½Π΄ΠΎΡΠ΅Π»ΠΈΠ°Π»ΡΠ½ΠΎΠ³ΠΎ ΡΠ°ΠΊΡΠΎΡΠ° ΡΠΎΡΡΠ° Π (VEGF-A), ΡΠ°ΡΡΠ²ΠΎΡΠΈΠΌΠΎΠΉ ΡΠΎΡΠΌΡ ΠΌΠΎΠ»Π΅ΠΊΡΠ»Ρ ΠΌΠ΅ΠΆΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠΉ Π°Π΄Π³Π΅Π·ΠΈΠΈ (s-ICAM-1) ΠΈ ΡΠ½Π΄ΠΎΡΠ΅Π»ΠΈΠ½Π°-1 (ET-1). ΠΡΠ΅Π΄ΠΏΠΎΠ»Π°Π³Π°Π΅ΡΡΡ, ΡΡΠΎ ΠΏΠΎΠ²ΡΡΠ΅Π½Π½ΡΠΉ ΡΡΠΎΠ²Π΅Π½Ρ VEGF-A, s-ICAM-1 ΠΈ ET-1 ΠΏΡΠΈ Π°Π»ΠΊΠΎΠ³ΠΎΠ»ΡΠ½ΠΎΠΌ ΡΠΈΡΡΠΎΠ·Π΅ ΠΏΠ΅ΡΠ΅Π½ΠΈ (ΠΠ¦Π) ΠΌΠΎΠΆΠ΅Ρ Π±ΡΡΡ Π²Π·Π°ΠΈΠΌΠΎΡΠ²ΡΠ·Π°Π½ ΡΠΎ ΡΡΡΠΎΠ΅Π½ΠΈΠ΅ΠΌ ΠΏΠΎΠ»ΠΈΠΌΠΎΡΡΠ½ΡΡ
Π»ΠΎΠΊΡΡΠΎΠ² ΠΏΡΠΎΠΌΠΎΡΠΎΡΠ½ΡΡ
ΠΎΠ±Π»Π°ΡΡΠ΅ΠΉ ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²ΡΡΡΠΈΡ
Π³Π΅Π½ΠΎΠ², ΡΡΠΎ Π² ΡΠ²ΠΎΡ ΠΎΡΠ΅ΡΠ΅Π΄Ρ ΠΌΠΎΠΆΠ΅Ρ ΡΠ²Π»ΡΡΡΡΡ Π³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΈΠΌ ΡΠ°ΠΊΡΠΎΡΠΎΠΌ ΡΠΈΡΠΊΠ° ΡΠ°Π·Π²ΠΈΡΠΈΡ ΡΠΈΡΡΠΎΠ·Π° ΠΏΠ΅ΡΠ΅Π½ΠΈ.Π¦Π΅Π»Ρ β ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΡ Π²Π·Π°ΠΈΠΌΠΎΡΠ²ΡΠ·Ρ Π½ΠΎΡΠΈΡΠ΅Π»ΡΡΡΠ²Π° Π²Π°ΡΠΈΠ°Π½ΡΠ½ΡΡ
ΡΠΎΡΠΌ ΠΏΠΎΠ»ΠΈΠΌΠΎΡΡΠ½ΡΡ
Π»ΠΎΠΊΡΡΠΎΠ², ΡΠ°ΡΠΏΠΎΠ»ΠΎΠΆΠ΅Π½Π½ΡΡ
Π² ΠΏΡΠΎΠΌΠΎΡΠΎΡΠ½ΡΡ
ΠΎΠ±Π»Π°ΡΡΡΡ
VEGF-A, ICAM-1 ΠΈ ET-1, Ρ ΡΡΠΎΠ²Π½Π΅ΠΌ ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²ΡΡΡΠΈΡ
Π±Π΅Π»ΠΊΠΎΠ² Π² ΡΡΠ²ΠΎΡΠΎΡΠΊΠ΅ ΠΊΡΠΎΠ²ΠΈ ΠΈ ΡΠΈΡΠΊΠΎΠΌ ΡΠ°Π·Π²ΠΈΡΠΈΡ ΠΠ¦Π.ΠΠ΅ΡΠΎΠ΄Ρ. ΠΡΠ½ΠΎΠ²Π½ΡΡ Π³ΡΡΠΏΠΏΡ ΡΠΎΡΡΠ°Π²ΠΈΠ»ΠΈ ΠΏΠ°ΡΠΈΠ΅Π½ΡΡ Ρ ΠΏΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠΉ Π·Π°Π²ΠΈΡΠΈΠΌΠΎΡΡΡΡ ΠΎΡ Π°Π»ΠΊΠΎΠ³ΠΎΠ»Ρ, ΠΎΡΡΠ³ΠΎΡΠ΅Π½Π½ΠΎΠΉ ΡΠΈΡΡΠΎΠ·ΠΎΠΌ ΠΏΠ΅ΡΠ΅Π½ΠΈ (ΠΠ¦Π, n=60). ΠΡΡΠΏΠΏΡ ΠΊΠΎΠ½ΡΡΠΎΠ»Ρ ΡΠΎΡΡΠ°Π²ΠΈΠ»ΠΈ Π»ΠΈΡΠ°, ΡΡΡΠ°Π΄Π°ΡΡΠΈΠ΅ Π°Π»ΠΊΠΎΠ³ΠΎΠ»ΡΠ½ΠΎΠΉ Π·Π°Π²ΠΈΡΠΈΠΌΠΎΡΡΡΡ, Π±Π΅Π· ΠΏΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΠΈ ΠΏΠ΅ΡΠ΅Π½ΠΈ (ΠΠ, n=24). ΠΠ΅ΡΠΈΠΎΠ΄ Π½Π°Π±Π»ΡΠ΄Π΅Π½ΠΈΡ ΡΠ°Π²Π½ΡΠ»ΡΡ ΠΏΠ΅ΡΠΈΠΎΠ΄Ρ Π³ΠΎΡΠΏΠΈΡΠ°Π»ΠΈΠ·Π°ΡΠΈΠΈ. Π‘ΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅ VEGF-A, s-ICAM-1 ΠΈ ET-1 Π² ΡΡΠ²ΠΎΡΠΎΡΠΊΠ΅ ΠΊΡΠΎΠ²ΠΈ ΠΎΡΠ΅Π½ΠΈΠ²Π°Π»ΠΈ ΠΏΠΎΡΡΠ΅Π΄ΡΡΠ²ΠΎΠΌ ΠΈΠΌΠΌΡΠ½ΠΎΡΠ΅ΡΠΌΠ΅Π½ΡΠ½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π°. Π Π°ΡΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ Π²Π°ΡΠΈΠ°Π½ΡΠ½ΡΡ
ΡΠΎΡΠΌ ΠΏΠΎΠ»ΠΈΠΌΠΎΡΡΠ½ΡΡ
Π»ΠΎΠΊΡΡΠΎΠ², ΡΠ°ΡΠΏΠΎΠ»ΠΎΠΆΠ΅Π½Π½ΡΡ
Π² ΠΏΡΠΎΠΌΠΎΡΠΎΡΠ½ΡΡ
ΠΎΠ±Π»Π°ΡΡΡΡ
Π³Π΅Π½ΠΎΠ² VEGF-A (rs699947 ΠΈ rs2010963), ICAM1 (rs281437) ΠΈ ET-1 (rs1800541), Π² ΠΈΡΡΠ»Π΅Π΄ΡΠ΅ΠΌΠΎΠΉ Π²ΡΠ±ΠΎΡΠΊΠ΅ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ Ρ ΠΏΠΎΠΌΠΎΡΡΡ ΠΏΠΎΠ»ΠΈΠΌΠ΅ΡΠ°Π·Π½ΠΎΠΉ ΡΠ΅ΠΏΠ½ΠΎΠΉ ΡΠ΅Π°ΠΊΡΠΈΠΈ Π² ΡΠ΅ΠΆΠΈΠΌΠ΅ ΡΠ΅Π°Π»ΡΠ½ΠΎΠ³ΠΎ Π²ΡΠ΅ΠΌΠ΅Π½ΠΈ.Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. Π Π°Π·Π²ΠΈΡΠΈΠ΅ Π°Π»ΠΊΠΎΠ³ΠΎΠ»ΡΠ½ΠΎΠ³ΠΎ ΡΠΈΡΡΠΎΠ·Π° ΠΏΠ΅ΡΠ΅Π½ΠΈ ΡΠΎΠΏΡΠΎΠ²ΠΎΠΆΠ΄Π°Π»ΠΎΡΡ Π·Π½Π°ΡΠΈΡΠ΅Π»ΡΠ½ΡΠΌ ΠΏΠΎΠ²ΡΡΠ΅Π½ΠΈΠ΅ΠΌ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΈ VEGF-A, s-ICAM-1 ΠΈ ET-1 Π² ΡΡΠ²ΠΎΡΠΎΡΠΊΠ΅ ΠΊΡΠΎΠ²ΠΈ. ΠΡΠΈ ΡΡΠΎΠΌ Π±ΡΠ»ΠΈ Π²ΡΡΠ²Π»Π΅Π½Ρ ΠΏΡΡΠΌΡΠ΅ ΠΊΠΎΡΡΠ΅Π»ΡΡΠΈΠΎΠ½Π½ΡΠ΅ ΠΎΡΠ½ΠΎΡΠ΅Π½ΠΈΡ ΠΌΠ΅ΠΆΠ΄Ρ Π·Π½Π°ΡΠ΅Π½ΠΈΡΠΌΠΈ ΠΊΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΠΈ VEGF-A, s-ICAM-1 ΠΈ ET-1 Π² ΡΡΠ²ΠΎΡΠΎΡΠΊΠ΅ ΠΊΡΠΎΠ²ΠΈ ΠΈ Π΄ΠΈΠ°ΠΌΠ΅ΡΡΠΎΠΌ ΠΏΠΎΡΡΠ°Π»ΡΠ½ΠΎΠΉ Π²Π΅Π½Ρ Ρ Π»ΠΈΡ Ρ ΡΠΈΡΡΠΎΠ·ΠΎΠΌ ΠΏΠ΅ΡΠ΅Π½ΠΈ. ΠΠ°ΡΠΈΠ΅Π½ΡΡ Ρ ΠΠ¦Π ΡΠ°ΡΠ΅ ΡΠ²Π»ΡΡΡΡΡ Π½ΠΎΡΠΈΡΠ΅Π»ΡΠΌΠΈ Π°Π»Π»Π΅Π»Ρ G Π»ΠΎΠΊΡΡΠ° rs1800541, ΡΠ°ΡΠΏΠΎΠ»ΠΎΠΆΠ΅Π½Π½ΠΎΠ³ΠΎ Π² ΠΏΡΠΎΠΌΠΎΡΠΎΡΠ΅ Π³Π΅Π½Π° ET-1, ΠΏΠΎ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ Ρ Π»ΠΈΡΠ°ΠΌΠΈ, ΡΡΡΠ°Π΄Π°ΡΡΠΈΠΌΠΈ ΠΠ Π±Π΅Π· ΠΏΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΠΈ ΠΏΠ΅ΡΠ΅Π½ΠΈ, ΡΡΠΎ ΡΠΎΠΏΡΡΠΆΠ΅Π½ΠΎ Ρ ΠΏΠΎΠ²ΡΡΠ΅Π½Π½ΡΠΌ ΡΠΈΡΠΊΠΎΠΌ ΡΠ°Π·Π²ΠΈΡΠΈΡ ΡΠΈΡΡΠΎΠ·Π° ΠΏΡΠΈ Π°Π»ΠΊΠΎΠ³ΠΎΠ»ΡΠ½ΠΎΠΉ Π·Π°Π²ΠΈΡΠΈΠΌΠΎΡΡΠΈ. ΠΠΎΡΠΈΡΠ΅Π»ΡΡΡΠ²ΠΎ Π°Π»Π»Π΅Π»Ρ Π‘ Π»ΠΎΠΊΡΡΠ° rs699947, Π° ΡΠ°ΠΊΠΆΠ΅ Π°Π»Π»Π΅Π»Ρ Π‘ Π»ΠΎΠΊΡΡΠ° rs2010963, ΡΠ°ΡΠΏΠΎΠ»ΠΎΠΆΠ΅Π½Π½ΡΡ
Π² ΠΏΡΠΎΠΌΠΎΡΠΎΡΠ΅ Π³Π΅Π½Π° VEGF, Π±ΡΠ»ΠΎ ΡΠ²ΡΠ·Π°Π½ΠΎ Ρ ΠΏΠΎΠ²ΡΡΠ΅Π½Π½ΡΠΌ ΡΡΠΎΠ²Π½Π΅ΠΌ VEGF-A ΠΏΡΠΈ ΠΠ¦Π ΠΏΠΎ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ Π½ΠΎΡΠΈΡΠ΅Π»ΡΠΌΠΈ Π΄Π°Π½Π½ΠΎΠ³ΠΎ Π°Π»Π»Π΅Π»Ρ Π² Π³ΡΡΠΏΠΏΠ΅ ΠΠ. ΠΡΠΎΠΌΠ΅ ΡΠΎΠ³ΠΎ, Π² Π³ΡΡΠΏΠΏΠ΅ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² Ρ ΠΠ¦Π Π½ΠΎΡΠΈΡΠ΅Π»ΠΈ Π°Π»Π»Π΅Π»Ρ Π‘, Π³ΠΎΠΌΠΎΠ·ΠΈΠ³ΠΎΡΠ½ΠΎΠ³ΠΎ Π³Π΅Π½ΠΎΡΠΈΠΏΠ° Π‘Π‘ ΠΈ Π³Π΅ΡΠ΅ΡΠΎΠ·ΠΈΠ³ΠΎΡΠ½ΠΎΠ³ΠΎ Π³Π΅Π½ΠΎΡΠΈΠΏΠ° GC Π»ΠΎΠΊΡΡΠ° rs2010963 ΠΏΠΎ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ Ρ Π½ΠΎΡΠΈΡΠ΅Π»ΡΠΌΠΈ Π°Π»Π»Π΅Π»Ρ G ΠΈΠ»ΠΈ Π³ΠΎΠΌΠΎΠ·ΠΈΠ³ΠΎΡΠ½ΠΎΠ³ΠΎ Π³Π΅Π½ΠΎΡΠΈΠΏΠ° GG ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²Π΅Π½Π½ΠΎ Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΠ·ΠΎΠ²Π°Π»ΠΈΡΡ ΠΏΠΎΠ²ΡΡΠ΅Π½Π½ΡΠΌ ΡΡΠΎΠ²Π½Π΅ΠΌ VEGF-A Π² ΡΡΠ²ΠΎΡΠΎΡΠΊΠ΅ ΠΊΡΠΎΠ²ΠΈ.ΠΠ°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅. ΠΠΎΡΠΈΡΠ΅Π»ΡΡΡΠ²ΠΎ Π°Π»Π»Π΅Π»Ρ G Π»ΠΎΠΊΡΡΠ° rs1800541 (ET-1) ΡΠ²Π»ΡΠ΅ΡΡΡ ΡΠ°ΠΊΡΠΎΡΠΎΠΌ ΡΠΈΡΠΊΠ° ΡΠ°Π·Π²ΠΈΡΠΈΡ ΡΠΈΡΡΠΎΠ·Π° ΠΏΠ΅ΡΠ΅Π½ΠΈ ΠΏΡΠΈ Π·Π»ΠΎΡΠΏΠΎΡΡΠ΅Π±Π»Π΅Π½ΠΈΠΈ Π°Π»ΠΊΠΎΠ³ΠΎΠ»Π΅ΠΌ. ΠΠΎΡΠΈΡΠ΅Π»ΡΡΡΠ²ΠΎ Π°Π»Π»Π΅Π»Ρ Π‘ Π»ΠΎΠΊΡΡΠ° rs699947, Π° ΡΠ°ΠΊΠΆΠ΅ Π°Π»Π»Π΅Π»Ρ Π‘ Π»ΠΎΠΊΡΡΠ° rs2010963, ΡΠ°ΡΠΏΠΎΠ»ΠΎΠΆΠ΅Π½Π½ΡΡ
Π² ΠΏΡΠΎΠΌΠΎΡΠΎΡΠ΅ Π³Π΅Π½Π° VEGF, ΠΌΠΎΠΆΠ΅Ρ ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΡΡ ΠΏΠΎΠ²ΡΡΠ΅Π½Π½ΡΠΉ ΡΡΠΎΠ²Π΅Π½Ρ VEGF-Π Π² ΡΡΠ²ΠΎΡΠΎΡΠΊΠ΅ ΠΊΡΠΎΠ²ΠΈ ΠΏΡΠΈ ΠΠ¦Π
Oxidative stress in the alcoholic liver disease
Parameters reflecting oxidative stress (OS) have been studied in 37 patients with alcoholic liver disease (ALD) during admission to the hospital and 2 weeks after the beginning of therapy. The patients were divided into 3 groups: alcoholic hepatitis (AH), alcoholic cirrhosis with hepatic insufficiency (the group C by the Child-Paquet scale) and terminal stage patients (subsequently died). All patients were characterized by a significant increase in plasma products of lipid peroxidation (conjugated diene and malondialdehyde) and a decrease of the ceruloplasmin level. The coefficient C OS significantly exceeded normal values both on admission and after the 2-week course of traditional therapy. This suggests an important role of the OS with ALD. Β© 2014 Pleiades Publishing, Ltd
Oxidative stress in the alcoholic liver disease
Parameters reflecting oxidative stress (OS) have been studied in 37 patients with alcoholic liver disease (ALD) during admission to the hospital and 2 weeks after the beginning of therapy. The patients were divided into 3 groups: alcoholic hepatitis (AH), alcoholic cirrhosis with hepatic insufficiency (the group C by the Child-Paquet scale) and terminal stage patients (subsequently died). All patients were characterized by a significant increase in plasma products of lipid peroxidation (conjugated diene and malondialdehyde) and a decrease of the ceruloplasmin level. The coefficient C OS significantly exceeded normal values both on admission and after the 2-week course of traditional therapy. This suggests an important role of the OS with ALD. Β© 2014 Pleiades Publishing, Ltd
Blood Content of Markers of Inflammation and Cytokines in Patients With Alcoholic Cardiomyopathy and Ischemic Heart Disease at Various Stages of Heart Failure
We conducted a comparative study of content proinflammatory cytokines, biomarkers of inflammatory process, biochemical indicators of congestive heart failure (CHF) and hemodynamic parameters in patients with alcoholic cardiomyopathy (ACMP) and ischemic heart disease (IHD) with various NYHA classes. We examined 62 men with ACMP (n = 45) and IHD (n = 17) and NYHA class III-IV CHF. Patients of both groups had lowered ejection fraction (EF), dilated cardiac chambers, and increased left ventricular (LV) myocardial mass index (MMI). Relative LV wall thickness was within normal limits but in the ACMP group it was significantly lower than in IHD group what corresponded to the eccentric type of myocardial hypertrophy. Higher NYHA class was associated with lower EF and larger end diastolic and end systolic LV dimensions. In ACMP it was also associated with larger dimension of the right ventricle while in IHD with substantially larger (by 30%) dimension of atria. Substantial amount of endotoxin found in blood plasma of patients with IHD corresponded to the conception of increased intestinal permeability of in CHF. Alcohol abuse was an aggravating factor of endotoxin transmission and its concentration in patients with ACMP was 3 times higher than in patients with IHD. Patients with ACMP had substantially elevated blood concentrations of interleukins (IL) 6, 8, 12, tumor necrosis factor alpha (TNF-alpha), and its soluble receptor s-TNF-R; they also had twofold elevation of C-reactive protein concentration. ACMP was associated with manifold rise of blood content of brain natriuretic peptide (BNP). Patients with IHD also had elevated blood concentrations of IL 6, 8 and 12 but their values were 1.5-2 times lower than ACMP group. Blood content of TNF-alpha and s-TNF-R in IHD group was within normal limits. Higher NYHA class in ACMP patients was associated with higher concentrations of IL 6 and 8, TNF-alpha, and BNP. In both groups of patients contents of IL-12, s-TNF-R, TGF-1 beta and factors of acute phase of inflammation did not reflect severity of CHF. Functional insufficiency of myocardium in IHD patients was best characterized by blood content of IL-6 while in ACMP patients of BNP
Blood Content of Markers of Inflammation and Cytokines in Patients With Alcoholic Cardiomyopathy and Ischemic Heart Disease at Various Stages of Heart Failure
We conducted a comparative study of content proinflammatory cytokines, biomarkers of inflammatory process, biochemical indicators of congestive heart failure (CHF) and hemodynamic parameters in patients with alcoholic cardiomyopathy (ACMP) and ischemic heart disease (IHD) with various NYHA classes. We examined 62 men with ACMP (n = 45) and IHD (n = 17) and NYHA class III-IV CHF. Patients of both groups had lowered ejection fraction (EF), dilated cardiac chambers, and increased left ventricular (LV) myocardial mass index (MMI). Relative LV wall thickness was within normal limits but in the ACMP group it was significantly lower than in IHD group what corresponded to the eccentric type of myocardial hypertrophy. Higher NYHA class was associated with lower EF and larger end diastolic and end systolic LV dimensions. In ACMP it was also associated with larger dimension of the right ventricle while in IHD with substantially larger (by 30%) dimension of atria. Substantial amount of endotoxin found in blood plasma of patients with IHD corresponded to the conception of increased intestinal permeability of in CHF. Alcohol abuse was an aggravating factor of endotoxin transmission and its concentration in patients with ACMP was 3 times higher than in patients with IHD. Patients with ACMP had substantially elevated blood concentrations of interleukins (IL) 6, 8, 12, tumor necrosis factor alpha (TNF-alpha), and its soluble receptor s-TNF-R; they also had twofold elevation of C-reactive protein concentration. ACMP was associated with manifold rise of blood content of brain natriuretic peptide (BNP). Patients with IHD also had elevated blood concentrations of IL 6, 8 and 12 but their values were 1.5-2 times lower than ACMP group. Blood content of TNF-alpha and s-TNF-R in IHD group was within normal limits. Higher NYHA class in ACMP patients was associated with higher concentrations of IL 6 and 8, TNF-alpha, and BNP. In both groups of patients contents of IL-12, s-TNF-R, TGF-1 beta and factors of acute phase of inflammation did not reflect severity of CHF. Functional insufficiency of myocardium in IHD patients was best characterized by blood content of IL-6 while in ACMP patients of BNP
PNPLA3 rs738409 associates with alcoholic liver cirrhosis but not with serum levels of IL6, IL10, IL8 or CCL2 in the Russian population
Introduction and aim: Polymorphic variant rs738409 within the PNPLA3 gene associates with alcoholic liver cirrhosis (ALC) in heavy drinkers of various ancestry but has not yet been established in the Russian population characterized by high incidence of ALC. PNPLA3 rs738409 involvement in the inflammatory process has been proposed as one of the mechanisms of liver dysfunction. Relationship between the PNPLA3 polymorphism and the biochemical markers of inflammation in patients with ALC remains unclear. The current study revealed the association between the rs738409 polymorphism, liver cirrhosis and serum cytokines in heavy drinkers in the Russian population. Materials and methods: The serum levels of IL6, IL10, IL8, and CCL2 along with PNPLA3 rs738409 polymorphism were determined in heavy drinkers (AA, n = 71) and heavy drinkers with diagnosed liver cirrhosis (ALC, n = 110). All of the recruited individuals were Caucasians and belonged to the Russian population. Results: Heavy drinkers carrying PNPLA3 rs738409 CG or CG+GG genotypes as compared with CC genotype carriers or G allele as compared with C allele carriers had significant risk of ALC. In ALC levels of interleukins and CCL2 increased as compared with AA. PNPLA3 rs738409 CC carriers had lower cirrhosis stage as compared with CG+GG carriers, however there were no differences of IL6, IL10, IL8 or CCL2 levels between G allele carriers and non-carriers in heavy drinkers. Conclusion: Thus, in the Russian population heavy drinkers carrying PNPLA3 rs738409 G allele are at higher risk of ALC, however the presence of rs738409 allele does not influence the serum cytokine levels. Β© 2020 FundaciΓ³n ClΓnica MΓ©dica Sur, A.C