30 research outputs found
Terapija endometritisa krmaÄa intrauterinom instilacijom preparata na bazi zida kvasca
On the basis of our investigations it was possible to conclude that intrauterine treatment of sows with puerperal uterine infections with sterile YCW (Yeast Cell Wall) resulted in significant clinical improvement. The percent of recidivism was the lowest (10%) in groups of sows treated with 10 and 20 g of YCW. The degree of bacterial CFU (Colony Forming Units) reduction in samples of sows uterine flushings following instillation of YCW (5, 10 and 20 g) was wery high and ranged from 1361 to 1444 times, while in sows treated with Lotagen 2% solution (100 mL) this parametar was only 32. At the moment of weaning, piglets from sows treated with 10 and 20 g of YCW were heavier when compared to the control and Lotagen group and their DBWG (Daily Body Weight Gain) was higher when compared to the Lotagen and control group. Treatment of sows by IU instillation of YCW did not influence the number of piglets in the next breeding cycle.Na osnovu rezultata naÅ”ih istraživanja bilo je moguÄe zakljuÄiti da tretman krmaÄa obolelih od puerperalnih infekcija materice, sterilnim preparatom dobijenim iz zida kvasca (YCW), ima za posledicu znaÄajan kliniÄki napredak. Procenat krmaÄa sa recidivom je bio najniži (10%) u grupi tretiranoj sa 10 i 20g YCW. Stepen redukcije broja bakterijskih kolonija (CFU) u uzorcima dobijenih iz materice krmaÄa posle tretmana YCW preparatom je bio veoma visok i kretao se u opsegu od 1361 do 1444 puta. Kod obolelih krmaÄa, tretiranih rastvorom Lotagena (2%), vrednost ovog parametra je bila svega 32. U momentu zaluÄenja, prasad krmaÄa tretiranih YCW preparatom u koliÄini od 10 i 20 g bila su teža u poreÄenju sa prasadima krmaÄa grupe tretirane Lotagenom i prasadima kontrolnih (neteretiranih) krmaÄa. Osim toga i njihovi proseÄni dnevni prirasti su bili veÄi. Tretman krmaÄa intrauterinom instilacijom preparata na bazi YCW nije negativno uticao na broj prasadi u sledeÄem reproduktivnom ciklusu
Laparoskopsko osemenjavanje ovaca u Republici Srbiji
This paper describes a procedure of laparoscopic insemination (LAI) performed in 42 Ile de France ewes with deep-frozen rams' semen. Frozen semen doses was imported from France, originating from two rams (A and B), and used intermittently in three LAI cycles on different females. The ewes were examined ultrasonographically for pregnancy, two months following insemination and data of lambing were recorded at the end. Total achieved lambing rate was 61.90% (26/42) and total number of lambs was 49 (prolificacy 1.88; 1.16 lambs per sheep included in LAI protocol). Three labs from 2 ewes were stillborn and one dyed at 10th day after lambing. Lambing rates for rams A and B were 53.85% (14/26) and 75.00% (12/16), respectively. Introduction of laparoscopic insemination of sheep with imported deep-frozen semen of top quality rams, gaining technical experience and achieving conception rate over 65% could make this technique acceptable in improving genetic potential of top quality sheep flocks.U radu je opisan postupak laparoskopskog osemenjavanja (LAO) primenjen kod 42 ovce rase Ile de France duboko zamrznutim semenom. Tokom tri ciklusa osemenjavanja naizmeniÄno je koriÅ”Äeno seme dva ovna (A i B), uvezeno iz Francuske. Nakon dva meseca od osemenjavanja ovce su pregledane na sjagnjenost ultrasonografskom metodom i analizirani su podaci o jagnjenju. Ostvarena je koncepcija od 61,90% (26/42) i dobijeno je ukupno 49 jaganjaca (indeks jagnjena 1,88 po ojagnjenoj ovci, odnosno, 1,16 jagnjeta po ovci ukljuÄenoj u protokol LAO). Tri jagnjeta od dve ovce bila su mrtvoroÄena i jedno jagnje je uginulo 10. dana nakon jagnjenja. Sjagnjenost od semena ovna A iznosila je 58,85% (14/26) a od ovna B 75,00% (12/16). UvoÄenje laparoskopskog osemenjavanja ovaca, uvoz duboko-zamrznutog semena vrhunskih ovnova, sticanje iskustva i ostvarenje koncepcije od preko 65% može ovu tehniku uÄiniti prihvatljivom u popravljanju genetskog potencijala kvalitetnih stada ovaca
Uvoz nerastova - kontrola semena i moguÄnost reklamacije
Serbia is one of the countries with the continuous import of breeding sows and boars. Boars are usually imported at the age of 4 to 6 months, in the period when the quality of the breeding males cannot be determined due to sexual immaturity (prepubertal and pubertal age). In this paper, the method and results of semen quality control in 40 imported young boars are described, and also the method of documenting the cause for action claim. In the case of suspicious semen quality it is necesseary to perform at least 3 consecutive controls in one month intervals in order to establish a final estimation of quality and usability of semen. Of 40 imported boars, 4 boars (10%) were subject of complaint due to: azoospermia (1 boar), absence or reduction of total and progressive motility, present sperm agglutination (2 boars), and increased number of pathological forms of spermatozoa (78%, 1 boar). Increased proportion of sperm with unstable chromatin structure (SCSA test - 33.2% and 37.1%) was established in two boars. To initiate the complaint it is necessary to have a sales contract that provides possibility for the reclamation, recognized methods of semen quality control and trustful business relationship between all interested parties.Srbija se svrstava u red zemalja sa kontinuiranim uvozom priplodnih nazimica i nerastova. Nerastovi se uglavnom uvoze u dobi od 4 do 6 meseci, u periodu kada se kvalitet priplodnjaka ne može pouzdano utvrditi usled polne nezrelosti (prepubertetsko ili pubertetska dob nerastiÄa). U ovom radu opisan je postupak i rezultati kontrole kvaliteta semena kod 40 mladih nerastova iz uvoza, kao i naÄin dokumentovanja razloga za pokretanje postupka reklamacije. Kod sumnjivog kvaliteta semena potrebno je izvrÅ”iti najmanje 3 uzastopne kontrole u razmaku od po mesec dana, kako bi se donela konaÄna ocena o kvalitetu semena i upotrebljivosti nerasta za priplod. Od 40 uvezenih nerastova, 4 su reklamirana (10%) usled: azoospermije (1 nerast), odsustva ili smanjenje ukupne ili progresivne pokretljivosti, uz prisustvo aglutinacija spermatozoida (2 nerasta), i poveÄanog broja patoloÅ”kih formi spermatozoida (78%; 1 nerast). Kod dva nerasta zabeležen je poveÄan udeo spermatozoida sa nestabilnom strukturom hromatina (SCSA test - 33,2% i 37,1%). Za pokretanje reklamacije neophodno je imati kupoprodajni ugovor koji predviÄa moguÄnost reklamacije, priznate metode kontrole semena kao i izgraÄen poslovni odnos poverenja zainteresovanih strana
Influence of the protein content of boar seminal plasma on spermatozoa viability, motility and acrosome integrity in diluted semen stored for 3 days
The aim of the present study was to investigate the influence of the protein content of seminal plasma on the motility, viability and acrosome integrity of spermatozoa in extended semen stored for 3 days. A total of 32 semen samples (from four boars) with high (4 mg/ml) and 32 semen samples (from four boars) with low (2 mg/ml) protein content were investigated. The semen samples were diluted by BTS at a ratio of 1:4, and stored for 72 h at 17oC. The percentages of live sperm (LS), live sperm with damaged acrosome (LDA) and total sperm with damaged acrosome (TDA) were detected by flow cytometry. Sperm progressive motility (PM) was detected using CASA. After 72 h of storage, the percentage of LS and PM was significantly (P lt 0.01) higher, and the LDA and TDA were significantly (P lt 0.01) lower in samples with high protein content than in the samples with low protein content (LS = 66 vs. 44%, PM = 64 vs. 48%, LDA = 15 vs. 21% and TDA = 29 vs. 45%, respectively). When comparing the difference between 0 and 72 h of storage, the percentage decrease in LS and PM, while increase in LDA and TDA were significantly higher in the samples with low (LS: 75 to 44%; PM: 68 to 48%; LDA: 11 to 21% and TDA: 23 to 45%) than in the samples with high protein content (LS: 78 to 66%; PM: 70 to 64%; LDA: 9 to 15% and TDA: 17 to 29%). We concluded that protein content in seminal plasma has a significant influence on progressive motility, viability and acrosome integrity in diluted semen stored for 3 days
Model saradnje repro-centara i laboratorije za reprodukciju u kontroli kvaliteta semena nerastova
In this article we presented procedures and results of boar semen quality control performed at the Scientific Veterinary Institute 'Novi Sad' based on continuous cooperation with the farms' centers for boar semen production. The data obtained by computer analysis (CASA-computer assisted sperm analysis), flow cytometry and cyto-morphologic examination were used for semen quality evaluation. The selected parameters were compared with the reproductive results in sows, such as: farrowing rate, number of piglets per litter, ratio of piglets born alive and stillborn piglets). Semen quality evaluation based on spermatozoa progressive motility, sperm concentration, morphological characteristics and chromatine structure damage were used to give recommendations for semen processing, dilution degree, prospective therapy of boars, or, at least, their culing. Analysis of semen was complemented with seasonal bacterial cultivation and controls in cases of sudden drop on semen quality. Separate files containing semen quality graphs and reproductive indicators for easier monitoring were created for every boar. Systematic semen analyses performed by the use of several modern methods, along with periodic bacteriological control, offer possibilities for reliable assessment of boars' semen quality.U ovom radu su opisani postupak i rezultati kontrole kvaliteta semena nerastova u NauÄnom institutu za veterinarstvo 'Novi Sad' na osnovu kontinuirane saradnje sa farmskim centrima za proizvodnju semena nerastova. U analizi kvaliteta semena je koriÅ”Äena kompjuterska analiza (CASA-computer assysted sperm analysis), protoÄna citometrija i cito-morfoloÅ”ki pregled. Odabrani parametri kvaliteta semena su uporeÄivani sa reproduktivnim parametrima krmaÄa kao Å”to su oprasivost, broj prasadi po leglu, odnos živoroÄene i mrtvoroÄene prasadi. Ocena kvaliteta semena na osnovu progresivne pokretljivosti, koncentracije spermatozoida, morfoloÅ”kih odlika i oÅ”teÄenja hromatina je koriÅ”Äena za davanje preporuka o naÄinu pripreme semena, odreÄivanje stepena razreÄenja ili sprovoÄenje eventualne terapije nerastova, odnosno, njihovog iskljuÄenja iz priploda. Ove analize semena se dopunjavaju sezonskim bakterioloÅ”kim pregledom i kontrolom u sluÄaju pada kvaliteta semena. Za svakog nerasta je otvorena kartoteka kvaliteta semena sa grafiÄkim prikazom i reproduktivnim pokazateljima, radi lakÅ”eg praÄenja. Kontinuirana sistematska analiza kvaliteta semena, kombinovana sa viÅ”e savremenih metoda, dopunjena povremenim bakterioloÅ”kim pregledom, daje moguÄnost pouzdane procene kvaliteta semena nerastova
Reproduktivna efikasnost visoko-mleÄnih krava posle telenja
The aim of this work was to determine incidence of calving and postcalving reproduction disorders and their effect on the major parameters of cows reproductive efficiency at dairy farm in the R. Serbia. Total number of 1835 high-milking dairy cows (av. milk production >8500L/305d of lactation) records has been reviewed during one year period, at the large dairy farm in area of Vojvodina (R. Serbia). Normal calving and puerperal period without health problems was observed at 583 cows (27,6%), while different abnormalities during and after calving were recorded in 1252 cows (72,4%). The highest percentages of registered peripartal disorders were dystocia and endometritis (62% and 64%, respectively), affecting almost the same percentage of cows. Peripartal disorders had statistically significant influence (P lt 0.05) on the increase of number of services/conception (3,5 vs. 2,7, reproductive disorders vs. healthy cows, respectively), prolongation of the interval from first postpartal insemination till conception (110 vs. 57 days, reproductive disorders vs. healthy cows, respectively), and increase of days open period (214 vs. 146 days, reproductive disorders vs. healthy cows, respectively). This results have confirmed increased incidence of reproduction disorders during calving and postcalving period that significantly reduces reproductive efficiency in high milking dairy cows after parturition.Istraživanja u mnogim zemljama, tokom poslednjih decenija, pokazuju permanentan pad reproduktivne efikasnosti krava visoke mleÄnosti. Cilj ovog rada je da se utvrdi da li i u kojoj meri, peripartalni poremeÄaji imaju uticaja na osnovne parametre reproduktivne efikasnosti krava u naÅ”im proizvodnim uslovima. Istraživanjem je obuhvaÄeno ukupno 1835 krava, tokom jedne godine, na jednoj velikoj farmi sa visokom proizvodnjom mleka (proseÄna proizvodnja >8500L/305 dana laktacije) u regionu Vojvodine (Republika Srbija). Od ukupnog broja ispitivanih krava, telenje je proÅ”lo bez poremeÄaja kod 583 krave (27,6%), a razliÄiti poremeÄaji tokom i posle telenja su ustanovljeni kod 1.252 krave (72,4%). NajÄeÅ”Äe ustanovljeni peripartalni poremeÄaji kod visoko mleÄnih krava su teÅ”ko teljenje (62%) i endometritis (64%). Peripartalni poremeÄaji imaju statistiÄki znaÄajan uticaj (P lt 0,05) na poveÄanje indeksa osemenjavanja (3,5 prema 2,7), produženje intervala od prvog do fertilnog osemenjavanja (57 prema 110 dana), kao i na produženje trajanja servis perioda (146 prema 214 dana), u poreÄenju sa kravama bez poremeÄaja. Dobijeni rezultati pokazuju da na farmama visoko mleÄnih krava poviÅ”ena uÄestalost peripartalnih reproduktivnih poremeÄaja koji znaÄajno utiÄu na smanjenje reproduktivne efikasnosti krava nakon partusa
Effect of proteine content in boar seminal plasma on the sperm motility in diluted semen stored for 3 days
Recently, it was frequently demonstrated that fertility of sows after artificially inseminated is lower than after mating. This is associated with a reduced fertilization capacity of overdiluted insemination doses. The aim of this study was to investigate the sperm motility in the semen samples, forming from the ejaculates with high or low protein content, stored in vitro on 17 degrees C for 3 days. Progressive motility was significantly higher (p lt 0.01) in the ejaculates with high, compared to the ejaculates with low protein content (82% vs. 76%). After 3 days of storage, in the1:4 dilution proportion, the average progressive motility was significantly (p lt 0.01) decreased in relation to this value in native semen from the boars with high (82% to 64%), as well from the boars with low protein content in seminal plasma (76% to 48%). However, the average diluted semen progressive motility was significantly greater (p lt 0.01) in the boars with high (64%), compared to the boars with low protein content in seminal plasma (48%). The number of good diluted semen samples (>= 65% progressive motility), was also significantly (p lt 0.01) greater in the boars with high (41%), compared to the boars with low protein content in seminal plasma (12%). These results show that seminal plasma proteins play an important role in maintaining the sperm progressive motility of diluted semen in vitro stored for 3 days
Quality and fertility of extended boar semen after prolonged transport
The aim of this study was to analyse semen quality parameters of fresh-extended porcine semen imported from Denmark
through prolonged importing regulations and transport and to analyse fertility performance in sows inseminated four days post semen
collection. Semen was analysed immediately upon the arrival (4 days post collection) using computer-assisted sperm analyses (CASA),
cytomorphology and flow cytometry, and sows were inseminated later the same day. The mean sperm concentration was 1718.09 Ā±
100.11 Ć 106, with average of 1193.40 Ā± 90.58 Ć 106 motile cells (69%) and 756.04 Ā± 70.57 Ć 106 progressive motile spermatozoa (43.58%)
in a dose, which was almost twice lower in value than producer certified-declared number of 2 billion of motile cells. Acrosome and
sperm membrane integrity test indicated high percentage of total damaged acrosome. Mean values of semen samples analysed by
sperm chromatin structure assay (SCSA) showed relatively high degree of chromatin damage. Membrane permeability test showed high
percentage of sperm with damaged membrane. Prolonged transport negatively affected the sperm quality in terms of both motility and
chromatin structure stability. Fertility of sows was affected by semen quality in terms of concentration and sperm motility parameters,
as well as ratio of live sperm cells population with damaged acrosom
Effects of prostaglandin and HCG on out of season oestrous synchronization and fertility and assessment of progesterone concentration for early pregnancy diagnosis in ewes
The study investigates the possibility of oestrus synchronization in ewes of MIS sheep population using prostaglandin (PG) outside the breeding season, serum progesterone concentration for pregnancy diagnosis and effect of hCG (human chorionic gonadotropin) on reproductive parameters. Prostaglandin was used to induce oestrus at the dose of 2.5 ml/ewe, administered intramuscularly in two injections (with 11 days interval). Responsive ewes were bred to rams and were divided into two groups, one of which was administered with hCG at the dose of 300 IU i/m 7th day post mating and the second group which was used as a control. Serum progesterone was measured on the day of prostaglandin administration and 17 days post mating. Oestrus response was 66.67%, lambing rate was 70%, average litter size was 1.7 lambs/ewe and twinning rate was 60%. Administration of hCG 7 days post mating did not improve fertility results. Lambing, prolificacy and twinning rates were higher in control than in hCG group, but no statistical difference was found between these groups. From a total of 20 ewes that were mated after PG treatment in 18 of them (90%), on the basis of progesterone concentration measured on the 17th day after mating and subsequent lambing results, gestation was confirmed or denied
Development of sexual behaviour in ram lambs and its correlation to serum testosterone
The drive to mate females is variable among the rams and can have a major impact on sheep production. Androgen testosterone
may be a key mediator within the expression of various morphological and behavioural traits in mammals, but the factors underlying
individual variation in circulating testosterone levels are poorly understood.
The aim of this study was to investigate the development of sexual behaviour patterns in ram lambs as well as the role of testosterone
in the expression of their libido.
Research was carried out on the sheep farm of the Institute for Animal Husbandry, Belgrade, Serbia. The study included 20 crossbred
ram lambs (autochthonous breed Pirot Pramenka x Merinolandschaf x Ile de France). All ram lambs used in the study were
of same age and rearing conditions. They were weaned at 2 months of age and kept indoors from birth throughout the whole
study. Animals were introduced in the study at the age of 3 months and the trial was completed at the ramsā age of 17 months.
Blood samples for testosterone levels were taken bimonthly (at the age of 3, 5, 7, 9, 11, 13, 15 and 17 months), as well as were
male-female and male-male interactions recorded.
Male-female interactions included the following elements of behaviour: nosing (or anogenital sniffing), pawing, flehmen response,
attempted mounts. Also, duration of all male activities directed towards ewe was recorded (male-female interactions in total).
Male-male interactions involved the frequency of male-male mounts.
Performed investigations showed that sexual behaviour of rams was age dependent, but poorly correlated to serum testosterone.
The average serum testosterone levels ranged from 1.83-13.28 ng/mL, and were age dependant (P<0.05).
Male-female oriented behaviour developed linearly with age, while male-male specific behaviour was characterized by high intensity
in young age and then pronounced variability in later test periods. None of the studied behavioural interactions were highly
correlated to serum testosterone. These findings support standpoint of more than one factor influencing development of sexual
behaviour of ram lambs