Terapija endometritisa krmača intrauterinom instilacijom preparata na bazi zida kvasca

On the basis of our investigations it was possible to conclude that intrauterine treatment of sows with puerperal uterine infections with sterile YCW (Yeast Cell Wall) resulted in significant clinical improvement. The percent of recidivism was the lowest (10%) in groups of sows treated with 10 and 20 g of YCW. The degree of bacterial CFU (Colony Forming Units) reduction in samples of sows uterine flushings following instillation of YCW (5, 10 and 20 g) was wery high and ranged from 1361 to 1444 times, while in sows treated with Lotagen 2% solution (100 mL) this parametar was only 32. At the moment of weaning, piglets from sows treated with 10 and 20 g of YCW were heavier when compared to the control and Lotagen group and their DBWG (Daily Body Weight Gain) was higher when compared to the Lotagen and control group. Treatment of sows by IU instillation of YCW did not influence the number of piglets in the next breeding cycle.Na osnovu rezultata naših istraživanja bilo je moguće zaključiti da tretman krmača obolelih od puerperalnih infekcija materice, sterilnim preparatom dobijenim iz zida kvasca (YCW), ima za posledicu značajan klinički napredak. Procenat krmača sa recidivom je bio najniži (10%) u grupi tretiranoj sa 10 i 20g YCW. Stepen redukcije broja bakterijskih kolonija (CFU) u uzorcima dobijenih iz materice krmača posle tretmana YCW preparatom je bio veoma visok i kretao se u opsegu od 1361 do 1444 puta. Kod obolelih krmača, tretiranih rastvorom Lotagena (2%), vrednost ovog parametra je bila svega 32. U momentu zalučenja, prasad krmača tretiranih YCW preparatom u količini od 10 i 20 g bila su teža u poređenju sa prasadima krmača grupe tretirane Lotagenom i prasadima kontrolnih (neteretiranih) krmača. Osim toga i njihovi prosečni dnevni prirasti su bili veći. Tretman krmača intrauterinom instilacijom preparata na bazi YCW nije negativno uticao na broj prasadi u sledećem reproduktivnom ciklusu

Laparoskopsko osemenjavanje ovaca u Republici Srbiji

This paper describes a procedure of laparoscopic insemination (LAI) performed in 42 Ile de France ewes with deep-frozen rams' semen. Frozen semen doses was imported from France, originating from two rams (A and B), and used intermittently in three LAI cycles on different females. The ewes were examined ultrasonographically for pregnancy, two months following insemination and data of lambing were recorded at the end. Total achieved lambing rate was 61.90% (26/42) and total number of lambs was 49 (prolificacy 1.88; 1.16 lambs per sheep included in LAI protocol). Three labs from 2 ewes were stillborn and one dyed at 10th day after lambing. Lambing rates for rams A and B were 53.85% (14/26) and 75.00% (12/16), respectively. Introduction of laparoscopic insemination of sheep with imported deep-frozen semen of top quality rams, gaining technical experience and achieving conception rate over 65% could make this technique acceptable in improving genetic potential of top quality sheep flocks.U radu je opisan postupak laparoskopskog osemenjavanja (LAO) primenjen kod 42 ovce rase Ile de France duboko zamrznutim semenom. Tokom tri ciklusa osemenjavanja naizmenično je korišćeno seme dva ovna (A i B), uvezeno iz Francuske. Nakon dva meseca od osemenjavanja ovce su pregledane na sjagnjenost ultrasonografskom metodom i analizirani su podaci o jagnjenju. Ostvarena je koncepcija od 61,90% (26/42) i dobijeno je ukupno 49 jaganjaca (indeks jagnjena 1,88 po ojagnjenoj ovci, odnosno, 1,16 jagnjeta po ovci uključenoj u protokol LAO). Tri jagnjeta od dve ovce bila su mrtvorođena i jedno jagnje je uginulo 10. dana nakon jagnjenja. Sjagnjenost od semena ovna A iznosila je 58,85% (14/26) a od ovna B 75,00% (12/16). Uvođenje laparoskopskog osemenjavanja ovaca, uvoz duboko-zamrznutog semena vrhunskih ovnova, sticanje iskustva i ostvarenje koncepcije od preko 65% može ovu tehniku učiniti prihvatljivom u popravljanju genetskog potencijala kvalitetnih stada ovaca

Uvoz nerastova - kontrola semena i mogućnost reklamacije

Serbia is one of the countries with the continuous import of breeding sows and boars. Boars are usually imported at the age of 4 to 6 months, in the period when the quality of the breeding males cannot be determined due to sexual immaturity (prepubertal and pubertal age). In this paper, the method and results of semen quality control in 40 imported young boars are described, and also the method of documenting the cause for action claim. In the case of suspicious semen quality it is necesseary to perform at least 3 consecutive controls in one month intervals in order to establish a final estimation of quality and usability of semen. Of 40 imported boars, 4 boars (10%) were subject of complaint due to: azoospermia (1 boar), absence or reduction of total and progressive motility, present sperm agglutination (2 boars), and increased number of pathological forms of spermatozoa (78%, 1 boar). Increased proportion of sperm with unstable chromatin structure (SCSA test - 33.2% and 37.1%) was established in two boars. To initiate the complaint it is necessary to have a sales contract that provides possibility for the reclamation, recognized methods of semen quality control and trustful business relationship between all interested parties.Srbija se svrstava u red zemalja sa kontinuiranim uvozom priplodnih nazimica i nerastova. Nerastovi se uglavnom uvoze u dobi od 4 do 6 meseci, u periodu kada se kvalitet priplodnjaka ne može pouzdano utvrditi usled polne nezrelosti (prepubertetsko ili pubertetska dob nerastića). U ovom radu opisan je postupak i rezultati kontrole kvaliteta semena kod 40 mladih nerastova iz uvoza, kao i način dokumentovanja razloga za pokretanje postupka reklamacije. Kod sumnjivog kvaliteta semena potrebno je izvršiti najmanje 3 uzastopne kontrole u razmaku od po mesec dana, kako bi se donela konačna ocena o kvalitetu semena i upotrebljivosti nerasta za priplod. Od 40 uvezenih nerastova, 4 su reklamirana (10%) usled: azoospermije (1 nerast), odsustva ili smanjenje ukupne ili progresivne pokretljivosti, uz prisustvo aglutinacija spermatozoida (2 nerasta), i povećanog broja patoloških formi spermatozoida (78%; 1 nerast). Kod dva nerasta zabeležen je povećan udeo spermatozoida sa nestabilnom strukturom hromatina (SCSA test - 33,2% i 37,1%). Za pokretanje reklamacije neophodno je imati kupoprodajni ugovor koji predviđa mogućnost reklamacije, priznate metode kontrole semena kao i izgrađen poslovni odnos poverenja zainteresovanih strana

Influence of the protein content of boar seminal plasma on spermatozoa viability, motility and acrosome integrity in diluted semen stored for 3 days

The aim of the present study was to investigate the influence of the protein content of seminal plasma on the motility, viability and acrosome integrity of spermatozoa in extended semen stored for 3 days. A total of 32 semen samples (from four boars) with high (4 mg/ml) and 32 semen samples (from four boars) with low (2 mg/ml) protein content were investigated. The semen samples were diluted by BTS at a ratio of 1:4, and stored for 72 h at 17oC. The percentages of live sperm (LS), live sperm with damaged acrosome (LDA) and total sperm with damaged acrosome (TDA) were detected by flow cytometry. Sperm progressive motility (PM) was detected using CASA. After 72 h of storage, the percentage of LS and PM was significantly (P lt 0.01) higher, and the LDA and TDA were significantly (P lt 0.01) lower in samples with high protein content than in the samples with low protein content (LS = 66 vs. 44%, PM = 64 vs. 48%, LDA = 15 vs. 21% and TDA = 29 vs. 45%, respectively). When comparing the difference between 0 and 72 h of storage, the percentage decrease in LS and PM, while increase in LDA and TDA were significantly higher in the samples with low (LS: 75 to 44%; PM: 68 to 48%; LDA: 11 to 21% and TDA: 23 to 45%) than in the samples with high protein content (LS: 78 to 66%; PM: 70 to 64%; LDA: 9 to 15% and TDA: 17 to 29%). We concluded that protein content in seminal plasma has a significant influence on progressive motility, viability and acrosome integrity in diluted semen stored for 3 days

Model saradnje repro-centara i laboratorije za reprodukciju u kontroli kvaliteta semena nerastova

In this article we presented procedures and results of boar semen quality control performed at the Scientific Veterinary Institute 'Novi Sad' based on continuous cooperation with the farms' centers for boar semen production. The data obtained by computer analysis (CASA-computer assisted sperm analysis), flow cytometry and cyto-morphologic examination were used for semen quality evaluation. The selected parameters were compared with the reproductive results in sows, such as: farrowing rate, number of piglets per litter, ratio of piglets born alive and stillborn piglets). Semen quality evaluation based on spermatozoa progressive motility, sperm concentration, morphological characteristics and chromatine structure damage were used to give recommendations for semen processing, dilution degree, prospective therapy of boars, or, at least, their culing. Analysis of semen was complemented with seasonal bacterial cultivation and controls in cases of sudden drop on semen quality. Separate files containing semen quality graphs and reproductive indicators for easier monitoring were created for every boar. Systematic semen analyses performed by the use of several modern methods, along with periodic bacteriological control, offer possibilities for reliable assessment of boars' semen quality.U ovom radu su opisani postupak i rezultati kontrole kvaliteta semena nerastova u Naučnom institutu za veterinarstvo 'Novi Sad' na osnovu kontinuirane saradnje sa farmskim centrima za proizvodnju semena nerastova. U analizi kvaliteta semena je korišćena kompjuterska analiza (CASA-computer assysted sperm analysis), protočna citometrija i cito-morfološki pregled. Odabrani parametri kvaliteta semena su upoređivani sa reproduktivnim parametrima krmača kao što su oprasivost, broj prasadi po leglu, odnos živorođene i mrtvorođene prasadi. Ocena kvaliteta semena na osnovu progresivne pokretljivosti, koncentracije spermatozoida, morfoloških odlika i oštećenja hromatina je korišćena za davanje preporuka o načinu pripreme semena, određivanje stepena razređenja ili sprovođenje eventualne terapije nerastova, odnosno, njihovog isključenja iz priploda. Ove analize semena se dopunjavaju sezonskim bakteriološkim pregledom i kontrolom u slučaju pada kvaliteta semena. Za svakog nerasta je otvorena kartoteka kvaliteta semena sa grafičkim prikazom i reproduktivnim pokazateljima, radi lakšeg praćenja. Kontinuirana sistematska analiza kvaliteta semena, kombinovana sa više savremenih metoda, dopunjena povremenim bakteriološkim pregledom, daje mogućnost pouzdane procene kvaliteta semena nerastova

Reproduktivna efikasnost visoko-mlečnih krava posle telenja

The aim of this work was to determine incidence of calving and postcalving reproduction disorders and their effect on the major parameters of cows reproductive efficiency at dairy farm in the R. Serbia. Total number of 1835 high-milking dairy cows (av. milk production >8500L/305d of lactation) records has been reviewed during one year period, at the large dairy farm in area of Vojvodina (R. Serbia). Normal calving and puerperal period without health problems was observed at 583 cows (27,6%), while different abnormalities during and after calving were recorded in 1252 cows (72,4%). The highest percentages of registered peripartal disorders were dystocia and endometritis (62% and 64%, respectively), affecting almost the same percentage of cows. Peripartal disorders had statistically significant influence (P lt 0.05) on the increase of number of services/conception (3,5 vs. 2,7, reproductive disorders vs. healthy cows, respectively), prolongation of the interval from first postpartal insemination till conception (110 vs. 57 days, reproductive disorders vs. healthy cows, respectively), and increase of days open period (214 vs. 146 days, reproductive disorders vs. healthy cows, respectively). This results have confirmed increased incidence of reproduction disorders during calving and postcalving period that significantly reduces reproductive efficiency in high milking dairy cows after parturition.Istraživanja u mnogim zemljama, tokom poslednjih decenija, pokazuju permanentan pad reproduktivne efikasnosti krava visoke mlečnosti. Cilj ovog rada je da se utvrdi da li i u kojoj meri, peripartalni poremećaji imaju uticaja na osnovne parametre reproduktivne efikasnosti krava u našim proizvodnim uslovima. Istraživanjem je obuhvaćeno ukupno 1835 krava, tokom jedne godine, na jednoj velikoj farmi sa visokom proizvodnjom mleka (prosečna proizvodnja >8500L/305 dana laktacije) u regionu Vojvodine (Republika Srbija). Od ukupnog broja ispitivanih krava, telenje je prošlo bez poremećaja kod 583 krave (27,6%), a različiti poremećaji tokom i posle telenja su ustanovljeni kod 1.252 krave (72,4%). Najčešće ustanovljeni peripartalni poremećaji kod visoko mlečnih krava su teško teljenje (62%) i endometritis (64%). Peripartalni poremećaji imaju statistički značajan uticaj (P lt 0,05) na povećanje indeksa osemenjavanja (3,5 prema 2,7), produženje intervala od prvog do fertilnog osemenjavanja (57 prema 110 dana), kao i na produženje trajanja servis perioda (146 prema 214 dana), u poređenju sa kravama bez poremećaja. Dobijeni rezultati pokazuju da na farmama visoko mlečnih krava povišena učestalost peripartalnih reproduktivnih poremećaja koji značajno utiču na smanjenje reproduktivne efikasnosti krava nakon partusa

Effect of proteine content in boar seminal plasma on the sperm motility in diluted semen stored for 3 days

Recently, it was frequently demonstrated that fertility of sows after artificially inseminated is lower than after mating. This is associated with a reduced fertilization capacity of overdiluted insemination doses. The aim of this study was to investigate the sperm motility in the semen samples, forming from the ejaculates with high or low protein content, stored in vitro on 17 degrees C for 3 days. Progressive motility was significantly higher (p lt 0.01) in the ejaculates with high, compared to the ejaculates with low protein content (82% vs. 76%). After 3 days of storage, in the1:4 dilution proportion, the average progressive motility was significantly (p lt 0.01) decreased in relation to this value in native semen from the boars with high (82% to 64%), as well from the boars with low protein content in seminal plasma (76% to 48%). However, the average diluted semen progressive motility was significantly greater (p lt 0.01) in the boars with high (64%), compared to the boars with low protein content in seminal plasma (48%). The number of good diluted semen samples (>= 65% progressive motility), was also significantly (p lt 0.01) greater in the boars with high (41%), compared to the boars with low protein content in seminal plasma (12%). These results show that seminal plasma proteins play an important role in maintaining the sperm progressive motility of diluted semen in vitro stored for 3 days

Quality and fertility of extended boar semen after prolonged transport

The aim of this study was to analyse semen quality parameters of fresh-extended porcine semen imported from Denmark through prolonged importing regulations and transport and to analyse fertility performance in sows inseminated four days post semen collection. Semen was analysed immediately upon the arrival (4 days post collection) using computer-assisted sperm analyses (CASA), cytomorphology and flow cytometry, and sows were inseminated later the same day. The mean sperm concentration was 1718.09 ± 100.11 × 106, with average of 1193.40 ± 90.58 × 106 motile cells (69%) and 756.04 ± 70.57 × 106 progressive motile spermatozoa (43.58%) in a dose, which was almost twice lower in value than producer certified-declared number of 2 billion of motile cells. Acrosome and sperm membrane integrity test indicated high percentage of total damaged acrosome. Mean values of semen samples analysed by sperm chromatin structure assay (SCSA) showed relatively high degree of chromatin damage. Membrane permeability test showed high percentage of sperm with damaged membrane. Prolonged transport negatively affected the sperm quality in terms of both motility and chromatin structure stability. Fertility of sows was affected by semen quality in terms of concentration and sperm motility parameters, as well as ratio of live sperm cells population with damaged acrosom

Effects of prostaglandin and HCG on out of season oestrous synchronization and fertility and assessment of progesterone concentration for early pregnancy diagnosis in ewes

The study investigates the possibility of oestrus synchronization in ewes of MIS sheep population using prostaglandin (PG) outside the breeding season, serum progesterone concentration for pregnancy diagnosis and effect of hCG (human chorionic gonadotropin) on reproductive parameters. Prostaglandin was used to induce oestrus at the dose of 2.5 ml/ewe, administered intramuscularly in two injections (with 11 days interval). Responsive ewes were bred to rams and were divided into two groups, one of which was administered with hCG at the dose of 300 IU i/m 7th day post mating and the second group which was used as a control. Serum progesterone was measured on the day of prostaglandin administration and 17 days post mating. Oestrus response was 66.67%, lambing rate was 70%, average litter size was 1.7 lambs/ewe and twinning rate was 60%. Administration of hCG 7 days post mating did not improve fertility results. Lambing, prolificacy and twinning rates were higher in control than in hCG group, but no statistical difference was found between these groups. From a total of 20 ewes that were mated after PG treatment in 18 of them (90%), on the basis of progesterone concentration measured on the 17th day after mating and subsequent lambing results, gestation was confirmed or denied

Development of sexual behaviour in ram lambs and its correlation to serum testosterone

The drive to mate females is variable among the rams and can have a major impact on sheep production. Androgen testosterone may be a key mediator within the expression of various morphological and behavioural traits in mammals, but the factors underlying individual variation in circulating testosterone levels are poorly understood. The aim of this study was to investigate the development of sexual behaviour patterns in ram lambs as well as the role of testosterone in the expression of their libido. Research was carried out on the sheep farm of the Institute for Animal Husbandry, Belgrade, Serbia. The study included 20 crossbred ram lambs (autochthonous breed Pirot Pramenka x Merinolandschaf x Ile de France). All ram lambs used in the study were of same age and rearing conditions. They were weaned at 2 months of age and kept indoors from birth throughout the whole study. Animals were introduced in the study at the age of 3 months and the trial was completed at the rams’ age of 17 months. Blood samples for testosterone levels were taken bimonthly (at the age of 3, 5, 7, 9, 11, 13, 15 and 17 months), as well as were male-female and male-male interactions recorded. Male-female interactions included the following elements of behaviour: nosing (or anogenital sniffing), pawing, flehmen response, attempted mounts. Also, duration of all male activities directed towards ewe was recorded (male-female interactions in total). Male-male interactions involved the frequency of male-male mounts. Performed investigations showed that sexual behaviour of rams was age dependent, but poorly correlated to serum testosterone. The average serum testosterone levels ranged from 1.83-13.28 ng/mL, and were age dependant (P<0.05). Male-female oriented behaviour developed linearly with age, while male-male specific behaviour was characterized by high intensity in young age and then pronounced variability in later test periods. None of the studied behavioural interactions were highly correlated to serum testosterone. These findings support standpoint of more than one factor influencing development of sexual behaviour of ram lambs