13 research outputs found
Serum sTREM-1 as a surrogate marker of treatment outcome in patients with peptic ulcer disease
Objective Soluble triggering receptor expressed on myeloid cells-1 (sTREM-1) is elevated in the gastric juice and in cultures of gastric mucosa of patients with peptic ulcer disease (PUD). Its application as a surrogate marker for the treatment of PUD was assessed. Methods From 138 eligible patients, 96 were enrolled; 50 with duodenal ulcer, 29 with gastric ulcer and 17 with chronic gastritis. Patients were endoscoped twice; once before treatment and once after treatment. Biopsy specimens were collected for histopathologic estimation of gastritis. Blood was sampled prior to each endoscopy. Serum was collected and sTREM-1 was measured by an enzyme immunoabsorbent assay (http://www. clinicaltrials.gov identifier NCT00534443). Results At the end of treatment sTREM-1 was either: (a) below the limit of detection (this occurred in 62 patients and it was accompanied by lacks signs of residual disease in 58 patients, 93.5%); or (b) above the limit of detection (this occurred in 17 patients and it was accompanied by residual disease in 14 patients, 82.3%) (p<0.0001). Odds ratio for complete healing of peptic ulcer with sTREM-1 below detection limit was 5.30 (95% CI: 1.89-14.83, p<0.001) compared to serum sTREM-1 above the limit of detection. Conclusions Serum sTREM-1 below detection limit may effectively distinguish patients who successfully completed therapy for PUD from those with residual disease and apply as a surrogate marker. © Springer Science+Business Media, LLC 2011
Gastric mucosa epithelial cell kinetics are differentiated by anatomic site and Helicobacter pylori infection
Changes in epithelial cell turnover related to Helicobacter pylori
infection may contribute to gastric cancer development. The response of
different anatomic sites of the gastric mucosa to H. pylori is not
known. We studied apoptosis and cell proliferation at the grater and
lesser curvature of the antrum and corpus, the fundus, and the cardia
from 9 H. pylori gastritis patients and 11 H. pylori-negative controls
with normal histology. Proliferation was highest at the major curve of
the antrum and lowest at the fundus, and apoptosis was highest at the
cardia and lowest at the major curve of the antrum in both H. pylori
gastritis and normal mucosa. Proliferation was significantly higher at
all anatomic sites, while apoptosis was significantly lower only at the
major and lesser curve of the corpus in H. pylori gastritis compared
with normal controls. Our data suggest that gastric mucosa epithelial
cell kinetics is differentiated by the anatomic site and H. pylori
infection
The prevalence of overgrowth by aerobic bacteria in the small intestine by small bowel culture: Relationship with irritable bowel syndrome
Objectives Many studies have linked irritable bowel syndrome (IBS) with small intestinal bacterial overgrowth (SIBO), although they have done so on a qualitative basis using breath tests even though quantitative cultures are the hallmark of diagnosis. The purpose of this study was to underscore the frequency of SIBO in a large number of Greeks necessitating upper gastrointestinal (GI) tract endoscopy by using quantitative microbiological assessment of the duodenal aspirate. Methods Consecutive subjects presenting for upper GI endoscopy were eligible to participate. Quantitative culture of aspirates sampled from the third part of the duodenum during upper GI tract endoscopy was conducted under aerobic conditions. IBS was defined by Rome II criteria. Results Among 320 subjects enrolled, SIBO was diagnosed in 62 (19.4%); 42 of 62 had IBS (67.7%). SIBO was found in 37.5% of IBS sufferers. SIBO was found in 60% of IBS patients with predominant diarrhea compared with 27.3% without diarrhea (P = 0.004). Escherichia coli, Enterococcus spp and Klebsiella pneumoniae were the most common isolates within patients with SIBO. A stepwise logistic regression analysis revealed that IBS, history of type 2 diabetes mellitus and intake of proton pump inhibitors were independently and positively linked with SIBO; gastritis was protective against SIBO. Conclusions Using culture of the small bowel, SIBO by aerobe bacteria is independently linked with IBS. These results reinforce results of clinical trials evidencing a therapeutic role of non-absorbable antibiotics for the management of IBS symptoms. © 2012 Springer Science+Business Media, LLC
Percutaneous endoscopic gastrostomy: Adequacy and quality of information given to decision-makers
Background/Aim: Nowadays percutaneous endoscopic gastrostomy (PEG) is
widely available, but patient-selection criteria and quality of informed
consent are debated. The aims of this retrospective study were to
evaluate the quality of information given to the decision-makers
(relatives) and determine the overall acceptance of the procedure by the
patients’ family. Methods: The relatives of patients with PEG were
interviewed by telephone, using a structured questionnaire. They (n =
55; 36% spouses, 34% children, 30% other) gave information about
themselves and the patient (34 males, 21 females, median age 69, range
16-92 years) who underwent PEG tube placement for eating disorders or
dysphagia. Results: At the time of evaluation 30/55 (54.6%) patients
had died. The cumulative median survival was significantly longer in
patients younger than 75 years by 58 days (p = 0.009). Relatives
believed that PEG could improve the patients’ quality of life (56%)
or/and the underlying disease. Although 93% of the decision-makers
considered that their opinion had been taken into account when the
procedure was done, 25% said that they had not adequately been informed
about alternative methods and the complications of the procedure (38%).
54% said that the procedure had improved the quality of life of the
family. Most of the decision-makers believed that their decision was
correct (87%) and they would recommend PEG (84%) to other patients
suffering from dysphagia. Conclusion: Though several decision-makers
were not satisfied with the quality of information given before informed
consent, the overall acceptance of the PEG placement for nutritional
support is high. Copyright (C) 2003 S. Karger AG, Basel
Evidence for the role of gastric mucosa at the secretion of soluble triggering receptor expressed on myeloid cells (strem-1) in peptic ulcer disease
Aim: To investigate the role of gastric mucosa at the secretion of sTREM-1 in peptic ulcer. Methods: Seventy two patients were enrolled; 35 with duodenal, 21 with gastric ulcer and 16 with chronic gastritis. Patients were endoscoped and gastric juice was aspirated. Patients with duodenal and gastric ulcer underwent a second endoscopy post-treatment. Biopsies were incubated in the absence/presence of endotoxins or gastric juice. Supernatants were collected and sTREM-1 and TNFα were measured by enzyme immunoabsorbent assays. Scoring of gastritis was performed before and after treatment according to updated Sydney score. Results: Patients with duodenal and gastric ulcer and those with chronic gastritis had similar scores of gastritis. sTREM-1 was higher in supernatants of tissue samples of H pylori-positive than of H pylori-negative patients with gastric ulcer. Median (± SE) sTREM-1 was found increased in supernatants of patients with gastric ulcer before treatment (203.21 ± 88.91 pg/1000 cells) compared to supernatants either from the same patients post-treatment (8.23 ± 5.79 pg/1000 cells) or from patients with chronic gastritis (6.21 ± 0.71 pg/1000 cells) (P < 0.001 and < 0.001, respectively). Similar differences for sTREM-1 were recorded among LPS-stimulated tissue samples of patients (P = 0.001). Similar differences were not found for TNFα. Positive correlations were found between sTREM-1 of supernatants from patients with both duodenal and gastric ulcer before treatment and the degree of infiltration of neutrophils and monocytes. Conclusion: sTREM-1 secreted by the gastric mucosa is an independent mechanism connected to the pathogenesis of peptic ulcer. sTREM-1 was released at the presence of H pylori from the inflamed gastric mucosa in the field of gastric ulcer. © 2007 WJG. All rights reserved
Implications for a role of interleukin-23 in the pathogenesis of chronic gastritis and of peptic ulcer disease
The present study aimed to investigate the role of gastric mucosa for
the secretion of interleukin (IL)-23 in chronic gastritis. One hundred
and one patients were enrolled; 47 with duodenal ulcer, 33 with gastric
ulcer and 31 with chronic gastritis. Biopsies were incubated in the
absence/presence of endotoxins. Supernatants were collected and IL-23
and IL-1 beta were measured by enzyme-linked immunosorbent assay.
Scoring of gastritis was performed according to the updated Sydney
score. Patients with duodenal and gastric ulcer and those with chronic
gastritis had similar scores of gastritis. IL-23 was higher in
supernatants of tissue samples of Helicobacter pylori-positive than of
H. pylori-negative patients. No differences were recorded in
concentrations of IL-23 and IL-1 beta between patients with duodenal
ulcer, gastric ulcer and chronic gastritis. Positive correlations were
found between IL-23 of patients with both duodenal and gastric ulcer and
chronic gastritis and the degree of infiltration of neutrophils and
monocytes. Similar correlations were observed between IL-23 and IL-1
beta. IL-23 secreted by the gastric mucosa could be implicated in the
pathogenesis of chronic gastritis. IL-23 was released in the presence of
H. pylori from the inflamed gastric mucosa and followed the kinetics of
IL-1 beta
Molecular assessment of differences in the duodenal microbiome in subjects with irritable bowel syndrome
Objective. Breath testing and duodenal culture studies suggest that a
significant proportion of irritable bowel syndrome (IBS) patients have
small intestinal bacterial overgrowth. In this study, we extended these
data through 16S rDNA amplicon sequencing and quantitative PCR (qPCR)
analyses of duodenal aspirates from a large cohort of IBS, non-IBS and
control subjects. Materials and methods. Consecutive subjects presenting
for esophagogastroduodenoscopy only and healthy controls were recruited.
Exclusion criteria included recent antibiotic or probiotic use.
Following extensive medical work-up, patients were evaluated for
symptoms of IBS. DNAs were isolated from duodenal aspirates obtained
during endoscopy. Microbial populations in a subset of IBS subjects and
controls were compared by 16S profiling. Duodenal microbes were then
quantitated in the entire cohort by qPCR and the results compared with
quantitative live culture data. Results. A total of 258 subjects were
recruited (21 healthy, 163 non-healthy non-IBS, and 74 IBS). 16S
profiling in five IBS and five control subjects revealed significantly
lower microbial diversity in the duodenum in IBS, with significant
alterations in 12 genera (false discovery rate < 0.15), including
overrepresentation of Escherichia/Shigella (p = 0.005) and Aeromonas (p
= 0.051) and underrepresentation of Acinetobacter (p = 0.024),
Citrobacter (p = 0.031) and Microvirgula (p = 0.036). qPCR in all 258
subjects confirmed greater levels of Escherichia coli in IBS and also
revealed increases in Klebsiella spp, which correlated strongly with
quantitative culture data. Conclusions. 16S rDNA sequencing confirms
microbial overgrowth in the small bowel in IBS, with a concomitant
reduction in diversity. qPCR supports alterations in specific microbial
populations in IBS