The Pregnancy Diagnosis in Buffalo Species: Laboratory Methods

Pregnancy diagnosis plays an important role in the reproduction management of ruminants since embryonicmortality has a substantial impact on the fertility of a herd. Most of the embryonic losses occur during the first days afterfertilization and during the process of implantation. So it is very important to discriminate, with an early pregnancydiagnosis, non-pregnant from pregnant animals. Hormone analysis to detect pregnancy may be utilize as a more simpletechnique as an alternative of rectal palpation or ultrasound. In the last years, a large polymorphic family of placentaexpressedproteins has been discovered in ruminant species and used for pregnancy diagnosis. Members of this familyare named pregnancy-associated glycoproteins (PAG), being synthesized in the mono-and binucleate cells of theruminant's trophectoderm. Part of them is released in the maternal blood circulation where they can be assayed bydifferent laboratory techniques. Due to large variety of expressed molecules and to large variations in the posttranslationalprocessing of the PAG, different immuno-systems present different ability to quantify the PAG released inblood. The assay of PAG can also bring very interesting information for researchers working in programs focused on thestudy of embryonic and fetal mortalities, as well as on embryo biotechnology, animal nutrition or infections diseasesresulting in pathologies affecting the pregnancy

Feedlot rearing versus pasture grazing enhances plasma leptin and insulin-like growth factor-1 concentrations but does not anticipate puberty in dairy buffalo (Bubalus bubalis) heifers

Plasma Leptin (LEP) and Insulin-like Growth Factor-1 (IGF-1), related to growth and puberty onset, were assayed in growing buffalo heifers from 8 up to 21 months of age, reared in feedlot (FR, n=13; initial body weight 132 ± 11 Kg) or grazing at pasture (PG, n = 13; 137 ± 12 Kg). Mean age at puberty was not different between the two groups (599.5 and 610.5 days, in FR and PG, respectively). At puberty, FR heifers showed higher BCS, and higher average daily weight gain (DWG) than PG ones (0.87 vs. 0.62 Kg/day), and were heavier (462 vs. 375 Kg). Negative correlation between DWG and age at puberty was significant for PG group. Plasma Inhibin-A increased in both groups one month before puberty. Plasma LEP and IGF-1 sharply increased 2 months before puberty (about 18 months old) in FR heifers only, which showed higher concentrations of both hormones than PG heifers. Plasma LEP levels correlated positively with body weight and IGF-1. Despite hormone levels were affected by rearing systems, being lower in heifers grazing at pasture, these latter experienced adequate growth and reproductive maturation. In fact, grazing heifers reached puberty at the same age of the feedlot reared ones, with shorter puberty–conception interval (51.3 vs. 67.2 days, for PG and FR groups, respectively) and no difference in conception rates (83.3 and 84.6%, for PG and FR groups respectively). The higher BW and DWG of FR heifers were due to body fat deposition, which did not anticipate the puberty onset

Energy homeostasis in rabbit does during pregnancy and pseudopregnancy

This study was conducted to evaluate the changing concentrations of metabolic hormones and metabolites in pregnant (P) and pseudopregnant (PP) rabbit does. Twenty-five New Zealand White rabbit does were submitted to artificial insemination (AI) and then classified as P (n = 15) or PP (n = 10). Blood samples were collected weekly until day 32 post AI. During pregnancy, leptin concentrations were greater on Days 14 and 21 (P < 0.05), while insulin was greater on days 21 and 32 post AI (P < 0.05) compared to PP does. The triiodothyronine/thyroxine (T3/T4) ratio was greater in the first and last week (P < 0.001); whereas, cortisol concentrations were greater in the last week of pregnancy and after parturition (P < 0.01) compared with that of PP does. Non-esterified fatty acids (NEFA) concentrations increased from day 7 until day 32 post AI (P < 0.05). Glucose concentrations were unchanged throughout pregnancy although concentrations were positively associated with litter size. These results indicate concentrations of hormones and metabolites change during pregnancy to ensure energy requirements are met for both the foetuses and the maternal tissues. Physiological hyperleptinemia, hyperinsulinemia, and changes in cortisol as well as thyroid hormones indicate there is an adaptation of metabolic functions induced by pregnancy. These adaptations could be mediated by gonadal steroids because changes mainly occur in the second half of pregnancy when the profile of the sex hormones differs between P and PP does

Approaches to Identify Pregnancy Failure in Buffalo Cows

Simple SummaryEmbryonic mortality and pregnancy failures still represent a major issue in domestic livestock production, particularly in dairy cattle. Despite the presence of extensive work in this research area, there is still no effective, accurate and practical method able to determine timing and viability of embryo specifically during early gestation. Indeed, technologies and techniques for predicting pregnancy success must continue to be developed. The aim of this work was to find the best strategy to diagnose pregnancy failures in buffalo cows in order to improve farm reproductive management. Among the methods compared in this study (ultrasonography, progesterone, PAGs), pregnancy-associated glycoproteins (PAGs) seem to be the best marker for predicting embryonic mortality between 25 and 40 days of gestation to be utilized as a diagnostic tool to improve reproductive management in buffalo farms.The aim of this work was to find the best strategy to diagnose pregnancy failures in buffalo. A total of 109 animals belonging to a buffalo herd subjected to a synchronization and artificial insemination (AI) program were enrolled in this study. Blood samples were collected at days 0, 14, 25, 28 and 40 after AI for the determination of progesterone (P4) and pregnancy-associated glycoproteins (PAGs) by the radioimmunoassay (RIA) method. Transrectal ultrasonography was performed on day 25, 28 and 40 after AI to monitor pregnancy. The animals included in the data analysis were assigned ex post in pregnant (n = 50) and mortality (n = 12) groups. By ultrasonography, the predictive sign of mortality was the heartbeat. At day 25, the PAGs concentration was significant in predicting embryonic mortality with respect to ultrasonography and P4, at the cut-off of 1.1 ng/mL. At day 28, either PAGs, at a cut-off of 2.2 ng/mL, or ultrasonography, with no detection of heartbeat, were highly predictive of embryonic mortality. PAGs were the best marker (p < 0.05) for predicting embryonic mortality between 25 and 40 days of gestation in buffalo. Its utilization as a diagnostic tool can influence management decisions in order to improve farm reproductive management

Effect of Goji Berry (Lycium barbarum) Supplementation on Reproductive Performance of Rabbit Does

Simple Summary Infectious diseases represent serious problems for the reproductive performance of livestock animals because they negatively affect not only the welfare of the animals, but also the profitability of the farm. Moreover, the European Community continues to promote the reduction of the use of antibiotics and hormones in animal breeding. In this context, it is necessary to find new nutritional approaches to reduce the negative energy balance, and at the same time, to reinforce the immune system of the animals. In this research, the effect of goji berry supplementation on the reproductive activity and productive performance of rabbits is evaluated. Lycium barbarum fruit is considered a nutraceutical natural product containing various biologically active substances that show health benefits for both humans and animals. In particular, the berry can modulate hormones and metabolites involved in energy balance and reproduction, stimulate and balance the immune system activity, contributing to the defense of the organism against pathogens. Our results suggest that the integration with goji berry in the rabbit diet at 1% affects the reproductive activity, influencing the pattern secretion of luteinizing hormone (LH) and estrogens, as well as the sexual receptivity. Moreover, the fruit induced a higher milk production, improving the productive performance of young rabbits. Goji berry shows a wide range of beneficial properties in human health, but only a few studies evaluated its effects in livestock animals. The objective of this research was to assess the effects of goji berry supplementation on the hormonal profile, productive, and reproductive performance of does. Two months before artificial insemination, 105 nulliparous does were randomly divided into three groups (n = 35) based on the dietary treatment: commercial diet (C), or a diet supplemented with either 1% (G1), or 3% (G3) of goji berry, respectively. The results showed that receptivity was higher in G1 than in the C group (p < 0.05). Trends toward significance for differences between the G1 and G3 groups in marginal means of LH concentrations (p = 0.059), and between G1 and C in LH AUC values (p = 0.078), were evidenced. Estrogen concentrations showed a more fluctuating trend but a significant interaction effect (p < 0.001). The G1 group showed higher litter weight than C at birth (p = 0.008) and weaning (p < 0.001), as well as higher litter size at weaning (p = 0.020). The G1 group also exhibited the highest mean milk production (p < 0.01). In conclusion, goji berry influenced reproductive and productive performance, probably via modulating hormonal patterns and milk production in rabbits. However, further studies are needed to validate these preliminary results

Using Pregnancy-Associated Glycoproteins (PAGs) to Improve Reproductive Management: From Dairy Cows to Other Dairy Livestock

Pregnancy success represents a major issue for the economic income of cattle breeders. Early detection of pregnant and non-pregnant animals, as well as the prediction of early pregnancy failure, can influence farm management decisions. Several diagnostic tools for pregnancy are currently available. Among these, pregnancy-associated glycoproteins (PAGs) have been shown to be useful for identifying the presence of vital embryos and for pregnancy follow-up monitoring. This review presents an overview of the PAGs’ functions, their pregnancy trends, and their use as a tool to improve reproductive management in bovine and other dairy livestock, such as small ruminants and buffalos

Relationship between oxidative stress and the success of artificial insemination in dairy cows in a pasture-based system

This study was designed to evaluate whether the outcome of artificial insemination (AI) was affected by the metabolic and oxidative status of dairy cows. Seventy-nine inseminations in 40 cows, were classified, on the basis of blood progesterone (P4) and pregnancy-associated glycoprotein (PAG) concentrations and clinical confirmation of pregnancy into, three categories: (1) positive (AI+, resulted in pregnancy, n = 26; 33%), (2) negative (AI, did not result in pregnancy, n = 49; 62%), and (3) embryonic mortality (EM, n = 4; 5%). Reactive oxygen metabolites, biological antioxidant potential, oxidative stress index, body condition score, glucose, total proteins, albumin, urea, non-esterified fatty acids (NEFAs), cholesterol, triglycerides, haptoglobin and advanced oxidative protein products (AOPPs) were measured on the day of AI (day 0), and 30 and 42 days later. Cows with EM had lower BCS scores (2.5) than AI+ (2.8) and AI (2.9) cows (P < 0.05). During the postpartum period, body condition score (BCS) increased and NEFAs decreased (P < 0.05) suggesting a recovery from the negative energy balance (NEB). The only significant differences found were that the mean concentration of AOPPs was higher and that of albumin lower in EM cows than in AI+ and AI (P < 0.05) animals. Plasma concentration of reactive oxygen metabolites and biological antioxidant potential were not related to AI outcome. Further studies are required to confirm this finding and to clarify the role of oxidative status on cows\u2019 fertility