Fermentation chemistry analysis of wines using HPLC.

<p>Detection Limit 0.1g/L * g/L, ≠ % v/v Levels not connected by same letter are significantly different (p<0.05).</p

Solvent-extractable volatile fermentation products of AWRI 838, CXM1 and CXM4 in Chardonnay wines.

<p>Levels not connected by same letter are significantly different (p<0.05).</p

Grape juice fermentation profile of AWRI 838 and hybrid strains CxM1-CxM5.

<p><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0062053#pone-0062053-g005" target="_blank">Figure 5a</a>. (top) Cell growth during fermentation as determined by Optical Density. Data points are presented with error bars. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0062053#pone-0062053-g005" target="_blank">Figure 5b</a>. (bottom) Sugar utilisation during fermentation as determined by Refractive Index. Data points are presented with error bars.</p

Primer sets and restriction endonucleases used to generate species-specific chromosomal markers.

<p>Primer sets and restriction endonucleases used to generate species-specific chromosomal markers.</p

Polyphenolic analysis of Chardonnay wines made by AWRI 838, CxM1 and CxM4 using UV Scan data: an index of Phenolic content.

<p>Levels not connected by same letter are significantly different (p<0.05).</p

Genetic stability of CxM4 fermentation isolates using chromosomal targeted PCR-RFLP.

<p>First gel Chromosome XIV left arm, second gel Chromosome XIV right arm, third gel Chromosome XVI left arm and fourth gel Chromosome XVI right arm. Fifth gel Chromosome XII left arm, sixth gel Chromosome XII right arm, seventh gel Chromosome XIV left arm. Lane 1 100 bp ladder, lane 2 AWRI838, Lane 3 NCYC2888, lane 4 DNA from both parents, lane 5 Hybrid CxM4, lanes 6 to 55 isolates 1 to 50. Arrows point to isolates with altered chromosomal content.</p

Genetic confirmation of cell hybridization by rDNA ITS PCR-RFLP.

<p>Lane 1 100 bp ladder, lane 2 AWRI838, lane 3 NCYC2888, lane 4 DNA from both parents, lanes 5–9 Hybrids CxM1-5.</p

Target volatile fermentation products of AWRI 838, CXM1 and CXM4 in Chardonnay wines.

<p>Levels not connected by same letter are significantly different (p<0.05).</p

Sample sets of array-CGH data for parents and hybrid strain CxM1.

<p>Within each panel of microarray data, each column contains the a-CGH data for a given strain while each row corresponds to a probe for a chromosomal location. The leftmost three panels show the data for probes to the S. cerevisiae genome, located on chromosome V (“YD’ followed by chromosome coordinate), XIV (‘YN”), and XVI (”YP”); the rightmost three panels show data for probes to various regions (contig “c” followed by contig number) of the <i>S. mikatae</i> genome. 838 is the <i>S. cerevisiae</i> parent strain, AWRI 1529 is the <i>S. mikatae</i> parent strain NCYC2888, and AWRI2526 is the hybrid strain CxM1. Red hybridisation intensities for a probe indicate the presence of that species’ genome region, while green hybridisation intensities indicate the absence of that species’ genome region. The reduced intensity of <i>S. mikatae</i> probes in the hybrid dataset indicates a reduced <i>S. mikatae</i> ploidy level relative to <i>S. cerevisiae</i>, within the hybrid genome.</p

Phenotypic assessment assay plates.

<p>Top row plates left to right; YEPD at temperatures 22°C, 4°C and 37°C. Bottom row plates left to right; YEP 25% glucose, YEPD 14% ethanol. Strains are plated in columns at 10 fold serial dilutions from top to bottom; columns 1–5 CxM5-CxM1 in descending order, column 6 NCYC2888, column 7 AWRI838.</p