Micrographs of two vesicle populations with different vesicle densities in solution.

<p>Vesicle abundance is higher in (a) than in (b). The lower abundance of vesicles (b) is preferred for better performance of the computer vision algorithms.</p

Vesicle measurements from three experiments; the experimental parameters for each experiment (time is the duration of incubation at recording; the number of chambers is the same as the number of samples).

<p>Relative standard deviation (RSD) measures the variation between chambers of the same populations. Each population had multiple chambers; <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0113405#pone-0113405-t001" target="_blank">Table 1</a> contains the mean vesicle quantity per chamber mean vesicle diameter and mean vesicle isoperimetric quotient (IPQ).</p><p>Vesicle measurements from three experiments; the experimental parameters for each experiment (time is the duration of incubation at recording; the number of chambers is the same as the number of samples).</p

Histograms of vesicle diameter size distribution (a) and vesicle isoperimetric quotient (b).

<p>Data samples for both histograms were taken from one randomly selected sample and their shapes are representative of all samples.</p

Scheme of the perfusion chambers (a).

<p>Silicone model with four perfusion chambers named C1–C4 (b). The side view is given in the figure and the depth of the chambers is 0.5 mm. A single chamber with two tracks (P1 and P2) locations of two tracks recorded in each chamber (c). The two tracks together cover approximately 3% of the chamber area.</p

Changes in samples from different vesicle populations over time (from 3 to 60 minutes).

<p>Each experiment was conducted using a new initial vesicle population and each sample point represents a single chamber. Vesicle quantities (a), mean projected diameter sizes (b), and mean isoperimetric quotients (c). The box plot consists of mean minimal and maximal values 25th 50th and 75th percentile.</p

Light micrograph of vesicles in a micrograph (a), vesicles with overlaid segmentation (b), mask with segmented vesicles separated from the background in which each vesicle is marked with a number (c).

<p>Light micrograph of vesicles in a micrograph (a), vesicles with overlaid segmentation (b), mask with segmented vesicles separated from the background in which each vesicle is marked with a number (c).</p