L vs. R Retroauricular Crease U-U Plot.

<p>This U-U plot represents the taxonomic differences between the L and R retroauricular crease samples collected from the cohort. The taxonomic ubiquity of the L and R retroauricular crease is represented along the x-axis and y-axis, respectively. If a U-U curve falls along the diagonal line with a slope of 1, then there is no difference between the ubiquities across the abundances for that taxon. The more significant the differences between the ubiquities of a taxon, the further off the diagonal its U-U curve will deviate. The most saliently deviating U-U curves are labeled. The p-value (0.158) for the AWKS statistic computed between the symmetric body habits indicate that there was not a statistically significant (α = 0.05) difference between the microbial communities of the two symmetric body habitats.</p

The Core Genera and OTUs at 1% | 80% and 10% | 75%.

<p>Corresponding core OTUs underlying the taxonomy are labeled in parentheses.</p><p>A bullet (•) identifies core family and genera at >75% ubiquity and >10% abundance.</p

R Retroauricular Crease vs.R Antecubital Fossa U-U Plot.

<p>This U-U plot represents the taxonomic differences between the two skin body habitats of R retroauricular crease and R antecubital fossa. The ubiquity of taxa for R retroauricular crease and R antecubital fossa are represented along the x-axis and y-axis, respectively. Taxonomic shifts toward the R antecubital fossa are represented by curves above the diagonal reference line. This plot reveals the greater taxonomic presence of <i>Staphylococcus</i>, <i>Propionibacterium</i>, <i>Anaerococcus</i>, and <i>Staphylococcaceae</i> (family) in the R retroauricular crease. The labeled points represent where the greatest difference of ubiquities are found. For example, at matching abundances, the maximum difference in ubiquity for <i>Streptococcus</i> was found at 76% of the cohort in R antecubital fossa, but only at 19% of the cohort in R retroauricular crease.</p

UPGMA Dendrogram of Stool Bacteroides OTUs.

<p>This is a UPGMA (average-linkage) dendrogram based on a distance matrix computed by using ANDES to estimate the RMSD between the nucleotide distributions of two groups of aligned sequences. The OTUs identified in the stool samples are colored blue. The reference sequences are labeled according to their Genbank ID and color-coded: green for the uncultured organisms and black for the cultured isolates. A short description for each reference sequence is labeled below the Genbank ID. A horizontal line was drawn to mark the possible cutoff for the RMSD difference separating species. The three novel branches and the three species-associated branches (<i>acidifaciens</i>-like, <i>massiliensis</i>-like and <i>vulgatus</i>-like) are marked with red dots.</p

Analyses of the Stability and Core Taxonomic Memberships of the Human Microbiome

<div><p>Analyses of the taxonomic diversity associated with the human microbiome continue to be an area of great importance. The study of the nature and extent of the commonly shared taxa (“core”), versus those less prevalent, establishes a baseline for comparing healthy and diseased groups by quantifying the variation among people, across body habitats and over time. The National Institutes of Health (NIH) sponsored Human Microbiome Project (HMP) has provided an unprecedented opportunity to examine and better define what constitutes the taxonomic core within and across body habitats and individuals through pyrosequencing-based profiling of 16S rRNA gene sequences from oral, skin, distal gut (stool), and vaginal body habitats from over 200 healthy individuals. A two-parameter model is introduced to quantitatively identify the core taxonomic members of each body habitat’s microbiota across the healthy cohort. Using only cutoffs for taxonomic ubiquity and abundance, core taxonomic members were identified for each of the 18 body habitats and also for the 4 higher-level body regions. Although many microbes were shared at low abundance, they exhibited a relatively continuous spread in both their abundance and ubiquity, as opposed to a more discretized separation. The numbers of core taxa members in the body regions are comparatively small and stable, reflecting the relatively high, but conserved, interpersonal variability within the cohort. Core sizes increased across the body regions in the order of: vagina, skin, stool, and oral cavity. A number of “minor” oral taxonomic core were also identified by their majority presence across the cohort, but with relatively low and stable abundances. A method for quantifying the difference between two cohorts was introduced and applied to samples collected on a second visit, revealing that over time, the oral, skin, and stool body regions tended to be more transient in their taxonomic structure than the vaginal body region.</p></div

Genera Minor Core for 2 Body Habitats.

<p>In these Ub-Ab plots, heavy bars are drawn vertically on the y-axis from 50% to 100% ubiquity, and horizontally on the log₁₀ transformed x-axis (abundance) from −4 to −2, representing an abundance of <1%. To qualify for greater than 50% ubiquity and less than 1% abundance, the Ub-Ab curve must originate from the range highlighted by the vertical bar and terminate on the range highlighted by the horizontal bar. The identified minor core taxa are identified for the tongue dorsum and stool body habitats.</p

Minor Core Taxa.

<p>Minor Core Taxa.</p

Palatine Tonsils, Second vs.First Visit U-U Plot.

<p>This U-U plot represents the comparison between the first and second visits for the palatine tonsils body habitat. There was a statistically significant difference between the two visits (p-value <0.001, α = 0.05).</p

OTU Ub-Ab Plots for 4 Body Habitats.

<p>These Ub-Ab plots were generated based on OTUs. The colored curves are based on the same colors used for the core genera in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0063139#pone-0063139-g001" target="_blank">Figure 1</a>. Lines with the same color belong to the same genera.</p

Variation of Abundance Plot.

<p>In these Var-Ab plots, the abundance is along the x-axis, and the variation is along the y-axis, with both measurements increasing towards the top right of the plot. Each glyph represents a single taxon, and its size is proportional to the ubiquity of the taxon, thus the larger the glyph, the more ubiquitous the taxon is across the cohort. Those taxa that were previously selected as core at 80% ubiquity and 1% abundance have been filled in with blue, and the minor core have been filled in with green. Taxa towards the top of the plot have greater variation than those on the bottom.</p