The role of AmtB, GlnK and glutamine synthetase in regulation of transcription factor tnra in bacillus subtilis

The nitrogen is a macroelement for all alive cells, from bacteria to animals. Although NH3/NH4 are highly toxic to animal, they are the preferred source of nitrogen for the most microorganisms and are assimilated by glutamine synthetase in the GOGAT cycle. The nitrogen limitation triggers a number of regulatory processes and activates many genes, providing the utilizing of alternative nitrogen sources. In Bacillus subtilis the genes of nitrogen metabolism are regulated by the transcription factor TnrA. In a cells it is bound to AmtB-GlnK proteins, the interaction with Glutamine synthetase (GS) represses its DNA-binding activity. Here we show the lack of AmtB leads to the nitrogen deficiency in a cell and, consequently, the increased expression of TnrA-de-pendent genes. In the lack of GlnK the transcription factor TnrA is constitutive bound to GS, the TnrA activity is repressed even under nitrogen limit conditions. Apparently, the TnrA activity is subjected to permanent repression by GS. In the absence of GS, the TnrA activity is strongly higher in compare to control, even under nitrogen limitation, when GS is active. These data allow to suggest that TnrA activity is regulated by the competitive binding to GlnK and GS

Significant delay of lethal outcome in cancer patients due to peroral administration of Bacillus oligonitrophilus KU-1.

Treatment of cancer patients remains a serious medical problem and the development of alternative treatment strategies is therefore of great importance. In this connection, we developed a new bacterial-based, anticancer method. Ten cancer patients (three males, seven females) were involved in this study. Bacterial suspension of stationary phase Bacillus oligonitrophilus KU-1 was used as a remedy for peroral administration. In five patients, side effects (sicchasia, slight blood, and intracranial pressure gain) were detected, but all patients showed significant delay of lethal outcome without serious side effects. In conclusion, the suggested method was, in our opinion, a good alternative to conventional chemo- and radiotherapy techniques. In order to evaluate its efficiency for various tumors, a double-blind, placebo-controlled, multicenter study is needed

Antitumor features of Bacillus oligonitrophilus KU-1 strain

Chemotherapy and radiotherapy remain mostly palliative methods for metastatic cancer treatment. Limitations in efficacy and safety of established treatments continue to underline the need for improved treatments for malignancy. Results with some probiotics with antitumor activity have been promising. Here, we report that oral reception of Bacillus oligonitrophilus KU-1 resulted in the prolongation of lives in cancer patients with terminal prognosis and stabilization of cancer growth. The theoretical basis for the phenomena observed is discussed

The monitoring of dirty electricity in A secondary school in kazan, republic of tatarstan, Russia

Electromagnetic fields from electronic equipment are detrimental environmental factors. Recently, a new type of electromagnetic pollution referred to as "dirty electricity" was discovered to affect human health. The current research measures levels of dirty electricity in one secondary school in Kazan, Republic of Tatarstan, Russia. A Microsurge II meter that measures high frequency transients and harmonics between 4 to 100 kHz (expressed as Graham-Stetzer units) was used in this study. Levels of dirty electricity were elevated in all areas of the school and the installation of Graham-Stetzer filters significantly reduced these levels. Taking into account the detrimental effects of the dirty electricity on human health, plugging one Graham-Stetzer filter into each classroom is highly recommended. © PSP Volume 18 - No 6. 2009

The monitoring of dirty electricity in A secondary school in kazan, republic of tatarstan, Russia

Electromagnetic fields from electronic equipment are detrimental environmental factors. Recently, a new type of electromagnetic pollution referred to as "dirty electricity" was discovered to affect human health. The current research measures levels of dirty electricity in one secondary school in Kazan, Republic of Tatarstan, Russia. A Microsurge II meter that measures high frequency transients and harmonics between 4 to 100 kHz (expressed as Graham-Stetzer units) was used in this study. Levels of dirty electricity were elevated in all areas of the school and the installation of Graham-Stetzer filters significantly reduced these levels. Taking into account the detrimental effects of the dirty electricity on human health, plugging one Graham-Stetzer filter into each classroom is highly recommended. © PSP Volume 18 - No 6. 2009