Characterization of Pasteurella multocida isolates recovered from the oral flora of cats

In this study, it was aimed to investigate the presence of Pasteurella multocida in the swab samples taken from the oral cavity of cats, and to determine the capsular type and antimicrobial susceptibility of the isolates. For this purpose, swab samples taken from 300 cats were inoculated onto Knight's selective enriched medium and blood agar for isolation of P. multocida. Following after capsular typing of the isolates by PCR, the susceptibilities of the isolates to ceftiofur, clindamycin, erythromycin, enrofloxacin, amoxicillin+clavulanic acid, trimethoprirn/ sulfamethoxazole, tetracycline, and chloramphenicol were examined by the disk diffusion method. The relationships between oral colonization and various physiological and behavioral variables were evaluated, statistically. P. multocida was isolated from 48 (16%) of the samples and all strains were typed as capsular type A. While all of the isolates were resistant to clindamycin and susceptible to enrofloxacin, 8,33% tetracycline resistance was also remarkable. Multiple antibiotic resistance (MDR) was detected in 27% of the isolates. The colonization of P. multocida was found to be statistically significant in cats under the age of 1 and in cats living outdoors. The importance of antibiotic resistance observed in bacteria with a zoonotic character such as P. multocida should not be ignored since it poses a threat to public health. It was concluded that, in this study determination of tetracycline resistance, which has started to be reported in feline Pasterurella multocida isolates in recent years, and detection of multiple antibiotic resistance in 27% of isolates, was extremely important for public health

Characterization of Pasteurella multocida isolates recovered from the oral flora of cats

In this study, it was aimed to investigate the presence of Pasteurella multocida in the swab samples taken from the oral cavity of cats, and to determine the capsular type and antimicrobial susceptibility of the isolates. For this purpose, swab samples taken from 300 cats were inoculated onto Knight's selective enriched medium and blood agar for isolation of P. multocida. Following after capsular typing of the isolates by PCR, the susceptibilities of the isolates to ceftiofur, clindamycin, erythromycin, enrofloxacin, amoxicillin+clavulanic acid, trimethoprirn/ sulfamethoxazole, tetracycline, and chloramphenicol were examined by the disk diffusion method. The relationships between oral colonization and various physiological and behavioral variables were evaluated, statistically. P. multocida was isolated from 48 (16%) of the samples and all strains were typed as capsular type A. While all of the isolates were resistant to clindamycin and susceptible to enrofloxacin, 8,33% tetracycline resistance was also remarkable. Multiple antibiotic resistance (MDR) was detected in 27% of the isolates. The colonization of P. multocida was found to be statistically significant in cats under the age of 1 and in cats living outdoors. The importance of antibiotic resistance observed in bacteria with a zoonotic character such as P. multocida should not be ignored since it poses a threat to public health. It was concluded that, in this study determination of tetracycline resistance, which has started to be reported in feline Pasterurella multocida isolates in recent years, and detection of multiple antibiotic resistance in 27% of isolates, was extremely important for public health

Otitis externa in dogs: Distribution and antimicrobial susceptibility patterns of Staphylococcus spp. isolates

The study aimed to investigate the Staphylococcus species from dogs with chronic otitis externa in Istanbul and to determine their antibiotic susceptibility patterns. Ear swab samples were collected from 100 dogs suspected of otitis externa admitted at the clinics of Istanbul University - Cerrahpaşa, Faculty of Veterinary Medicine. The dogs were of different age, gender, and breed. The bacterial isolation was performed by conventional methods. BD Phoenix Automated Microbiology System was used to confirm bacterial identification by conventional methods and to test antimicrobial susceptibility. Staphylococcus spp. were isolated from 36% of the samples collected from the dogs. S. pseudintermedius, S. aureus, S. epidermidis, S. hyicus and S. chromogenes were identified in 41.6%, 22.2%, 11.1%, 5.5%, and 5.5%. In Staphylococcus spp. isolates, enrofloxacin, penicillin, and ampicillin-sulbactam resistance was 8.3%, marbofloxacin resistance was 11.1%, doxycycline resistance was 16.6%, amoxicillin-clavulanic acid, erythromycin, and gentamicin resistance was 19.4%, tetracycline, clindamycin, and sulphonamide resistance was 25%. Methicillin resistance was not observed in any of the isolates. However, multiple drug resistance (MDR) was detected in 11 (30.5%) of 36 isolates. In conclusion, the early detection and antimicrobial sensitivity testing of Staphylococcus spp in dog otitis externa cases that do not respond to empiric therapy could be beneficial for appropriate antibiotic selection and treatment thus preventing MDR