Transcriptome Analysis of the Hepatopancreas in the Pacific White Shrimp (<i>Litopenaeus vannamei</i>) under Acute Ammonia Stress - Fig 1

<p>(a) GO categorization of non-redundant unigenes from the <i>de novo</i> assembly of LV_E and LV_C reads, and each annotated unigene was assigned at least one GO term. (b) KOG annotation of putative proteins. (c) KEGG classification of non-redundant unigenes.</p

The hierarchical clustering for the differentially expressed genes between LV_E and LV_C.

<p>The red color shows the high expression, and the blue color represents the down expression. The color from red to blue represents the log10 (FPKM+1) from large to small.</p

List of the genes in significantly changed KEGG pathways under acute ammonia stress in <i>Litopenaeus vannamei</i>.

<p>List of the genes in significantly changed KEGG pathways under acute ammonia stress in <i>Litopenaeus vannamei</i>.</p

Validation results of RNA-seq profiles by real time PCR.

<p>Validation results of RNA-seq profiles by real time PCR.</p

List of the candidate genes have homologies with known proteins in <i>Litopenaeus vannamei</i> under ammonia stress.

<p>List of the candidate genes have homologies with known proteins in <i>Litopenaeus vannamei</i> under ammonia stress.</p

The hierarchical clustering for the key differentially expressed genes between LV_E and LV_C from functional enrichment analysis of GO and KEGG pathways.

<p>The red color shows the high expression, and the blue color represents the down expression. The color from red to blue represents the log10 (FPKM+1) from large to small.</p

Transcriptome Analysis of the Hepatopancreas in the Pacific White Shrimp (<i>Litopenaeus vannamei</i>) under Acute Ammonia Stress - Fig 4

<p>The six significant changed KEGG pathways and two nitrogen metabolism pathways: a, Linoleic acid metabolism (ko00591); b, Inflammatory mediator regulation of TRP channels (ko04750); c, Arachidonic acid metabolism (ko00590); d, Serotonergic synapse (ko04726); e, Ovarian steroidogenesis (ko04913); f, Complement and coagulation cascades (ko04610); g, nitrogen metabolism pathway (ko00910); h, alanine, aspartate and glutamate metabolism pathway (ko00250). The green frames represents the genes were down-regulated, and the red frames represents the genes were up-regulated.</p

Summary of <i>de novo</i> assembly results of the transcriptome in hepatopancreas of <i>Litopenaeus vannamei</i>.

<p>Summary of <i>de novo</i> assembly results of the transcriptome in hepatopancreas of <i>Litopenaeus vannamei</i>.</p

Summary of the annotations of the unigenes under ammonia stress in <i>Litopenaeus vannamei</i>.

<p>Summary of the annotations of the unigenes under ammonia stress in <i>Litopenaeus vannamei</i>.</p