Association between MTHFR C677T and A1298C Polymorphisms and NSCL/P Risk in Asians: A Meta-Analysis

<div><p>Objective</p><p>Several studies have reported the association between methylenetetrahydrofolate reductase (MTHFR) C677T and A1298C polymorphisms and nonsyndromic cleft lip with or without palate (NSCL/P) in Asian populations. However, findings have been conflicting. In order to investigate the association, a meta-analysis was performed.</p><p>Methods</p><p>We searched Pubmed, MedLine and EmBase database to selected eligible studies. The pooled odds ratios (ORs) with 95% confidence intervals (95%CIs) were calculated using fixed effects model or random effects model to assess the association between MTHFR polymorphisms and NSCL/P in both Asian children and mothers.</p><p>Results</p><p>Finally, nine case-control studies were included. Overall, the MTHFR C677T polymorphism and NSCL/P showed pooled ORs (95%CI) of 1.41(1.23–1.61) in Asian children, and 1.70(1.19–2.42) in Asian mothers. Subgroup analyses by geographical locations further identified the association in Eastern Asian children, Western/Central Asian children and mothers, but not in Eastern Asian mothers. However, no significant relationship between MTHFR A1298C polymorphism and NSCL/P was found in this meta-analysis.</p><p>Conclusions</p><p>The MTHFR 677T allele was associated with an increased risk of NSCL/P in Asian populations.</p></div

Overall and subgroup results of the association between MTHFR C677T polymorphism and NSCL/P.

<p>Overall and subgroup results of the association between MTHFR C677T polymorphism and NSCL/P.</p

Funnel plot analysis to detect publication bias.

<p>Funnel plot analysis to detect publication bias.</p

Forest plot of association between MTHFR C677T polymorphism and NSCL/P risk in children (T vs. C).

<p>Forest plot of association between MTHFR C677T polymorphism and NSCL/P risk in children (T vs. C).</p

Overall and subgroup results of the association between MTHFR A1298C polymorphism and NSCL/P.

<p>Overall and subgroup results of the association between MTHFR A1298C polymorphism and NSCL/P.</p

Forest plot of association between MTHFR A1298C polymorphism and NSCL/P risk in mothers (T vs. C).

<p>Forest plot of association between MTHFR A1298C polymorphism and NSCL/P risk in mothers (T vs. C).</p

Flow chart explaining the selection of eligible studies included in the meta-analysis.

<p>Flow chart explaining the selection of eligible studies included in the meta-analysis.</p

The characteristics of eligible studies included in the meta-analysis.

<p><b>HWE</b> Hardy-Weinberg equilibrium; <b>HB</b> hospital based; <b>PB</b> population based.</p>a<p>two groups of control.</p

Flowchart of the process used for selection of eligible studies.

<p>Flowchart of the process used for selection of eligible studies.</p

Effect of Single Nucleotide Polymorphism Rs189037 in ATM Gene on Risk of Lung Cancer in Chinese: A Case-Control Study

<div><p>Background</p><p>Accumulated evidence has indicated that ataxia-telangiectasia mutated (ATM) gene polymorphisms are closely related to lung cancer. We aimed to explore the prognostic value of rs189037 (G>A), one of ATM single nucleotide polymorphisms (SNPs), and detect whether it involves in the risk of lung cancer in Chinese Han people.</p><p>Methods</p><p>In this hospital-based matched case-control study, 852 lung cancer patients and 852 healthy controls have been put into comparison to analyze the association between rs189037 and lung cancer risk in Chinese. The single nucleotide polymorphisms were determined by TaqMan real-time PCR and we used SPSS software to perform the statistical analyses.</p><p>Results</p><p>Individuals carrying variant AA genotype of rs189037 had higher lung cancer risk (adjusted OR: 1.56) than those carrying GG genotype. After analyzing data respectively from different groups divided by genders and smoking status, we observed that the risk effect of AA genotype on the lung cancer was significant in females, non-smokers and female non-smokers, as well as the risk effect of GA genotype in male smokers. Compared with non-smokers carrying GG genotype, smokers carrying at least one A allele had higher risk of developing lung cancer than those with GG genotype (adjusted OR: 3.52 vs. adjusted OR: 2.53).</p><p>Conclusions</p><p>This study suggested that rs189037 (G>A) polymorphism is associated with lung cancer risk in Chinese Han population. AA genotype and A allele may be dangerous lung cancer signals in Chinese and make contribution to diagnostic and treatment value.</p></div