22 research outputs found
Antidepressant Mechanism Research of Acupuncture: Insights from a Genome-Wide Transcriptome Analysis of Frontal Cortex in Rats with Chronic Restraint Stress
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Anatomical brain difference of subthreshold depression in young and middle-aged individuals
Background: Subthreshold depression (StD) is associated with substantial functional impairments due to depressive symptoms that do not fully meet the diagnosis of major depressive disorder (MDD). Its high incidence in the general population and debilitating symptoms has recently put it at the forefront of mood disorder research. Aim In this study we investigated common volumetric brain changes in both young and middle-aged StD patients. Methods: Two cohorts of StD patients, young and middle-aged, (n = 57) and matched controls (n = 76) underwent voxel-based morphometry (VBM). Results: VBM analysis found that: 1) compared with healthy controls, StD patients showed decreased gray matter volume (GMV) in the bilateral globus pallidus and precentral gyrus, as well as increased GMV in the left thalamus and right rostral anterior cingulate cortex/medial prefrontal cortex; 2) there is a significant association between Center for Epidemiological Studies Depression Scale scores and the bilateral globus pallidus (negative) and left thalamus (positive); 3) there is no interaction between age (young vs. middle-age) and group (StD vs. controls). Conclusions: Our findings indicate significant VBM brain changes in both young and middle-aged individuals with StD. Individuals with StD, regardless of age, may share common neural characteristics
TRAJECTORIES FOR SASAKIAN MAGNETIC FIELDS ON HOMOGENEOUS REAL HYPERSURFACES IN COMPLEX SPACE FORMS
Variation Characteristics of Ecosystem Water Use Efficiency and Its Response to Human Activity and Climate Change in Inner Mongolia
Water use efficiency (WUE) reflects the balance between carbon assimilation and water consumption in terrestrial ecosystems. Considering the fragile conditions of global water resources, the evaluation of regional WUE variation characteristics and response mechanisms is critical for promoting sustainable ecological development and water resource utilization. Based on gross primary productivity (GPP) and evapotranspiration (ET) datasets, combined with vegetation and meteorological data, this study examined the spatiotemporal variations, annual variation contribution rate, and driving mechanism of WUE in Inner Mongolia from 2001 to 2020. The main results are as follows: (1) The interannual and spatial variations of GPP, ET, and WUE all exhibited increasing trends, with WUE increasing in approximately 70% of the region and significantly increasing in 22.35% of pixels exhibiting a significant increase. Areas with the most significant increases were located in the Horqin sandy land and Mu Us sandy land. (2) The highest WUE values were observed in the summer season, followed by autumn and winter, and the lowest in spring. (3) Among all vegetation types, the typical steppe ecosystems contributed most to the interannual variability (IAV) of GPP, ET, and WUE, with values of 169.89%, 141.09%, and 193.42%, respectively. While the coniferous forest contributed least or negatively to GPP, ET, and WUE IAV, with values of −36.28%, 28.20%, and −32.86%, respectively. (4) The primary driver of WUE variation was found to be GPP, which contributed 59.36%, mainly in the central and western regions. The remaining 40.64% was attributable to ET, concentrated in the northeast region. (5) Human activities significantly affected WUE, with a contribution (about 53.52%) larger than that of climate change (nearly 46.48%). Increased precipitation improves vegetation WUE and is the most important climate factor influencing WUE variations. These findings will aid the formulation of vegetation protection and water resource management strategies in water-stressed areas
Antidepressant-Like Effects of Acupuncture-Insights From DNA Methylation and Histone Modifications of Brain-Derived Neurotrophic Factor
Sensitive and stable biomarkers that facilitate depression detection and monitor the antidepressant efficiency are currently unavailable. Thus, the objective is to investigate the potential of DNA methylation and histone modifications of brain-derived neurotrophic factor (BDNF) in monitoring severity and antidepressive effects of acupuncture. The depression rat model was imitated by social isolation and chronic unpredicted mild stress (CUMS). The expression of serum BDNF was detected by enzyme-linked immunosorbent assay (ELISA), the hippocampal BDNF, acetylation levels in histone H3 lysine 9 (acH3K9), and HDAC2 by Western blot, the hippocampal mRNA of BDNF by RT-polymerase chain reaction (PCR). The DNA methylation patterns of the promoter I of BDNF was detected by MS-PCR. We investigated that the expression of BDNF in serum and hippocampus were significantly downregulated compared with controls. The same trend was found in mRNA of BDNF. Notably, acupuncture reversed the downregulation of BDNF in serum and hippocampus and mRNA of BDNF compared with model group. Acupuncture reversed the CUMS-induced downregulation of hippocampal acH3K9. On the contrary, the CUMS-induced upregulation of hippocampal HDAC2 in model group was significantly reversed by acupuncture. Collectively, the antidepressant effect of acupuncture might be mediated by regulating the DNA methylation and histone modifications of BDNF, which may represent novel biomaker for detection of depression and monitoring severity and antidepressive effects
Variation Characteristics of Ecosystem Water Use Efficiency and Its Response to Human Activity and Climate Change in Inner Mongolia
Water use efficiency (WUE) reflects the balance between carbon assimilation and water consumption in terrestrial ecosystems. Considering the fragile conditions of global water resources, the evaluation of regional WUE variation characteristics and response mechanisms is critical for promoting sustainable ecological development and water resource utilization. Based on gross primary productivity (GPP) and evapotranspiration (ET) datasets, combined with vegetation and meteorological data, this study examined the spatiotemporal variations, annual variation contribution rate, and driving mechanism of WUE in Inner Mongolia from 2001 to 2020. The main results are as follows: (1) The interannual and spatial variations of GPP, ET, and WUE all exhibited increasing trends, with WUE increasing in approximately 70% of the region and significantly increasing in 22.35% of pixels exhibiting a significant increase. Areas with the most significant increases were located in the Horqin sandy land and Mu Us sandy land. (2) The highest WUE values were observed in the summer season, followed by autumn and winter, and the lowest in spring. (3) Among all vegetation types, the typical steppe ecosystems contributed most to the interannual variability (IAV) of GPP, ET, and WUE, with values of 169.89%, 141.09%, and 193.42%, respectively. While the coniferous forest contributed least or negatively to GPP, ET, and WUE IAV, with values of −36.28%, 28.20%, and −32.86%, respectively. (4) The primary driver of WUE variation was found to be GPP, which contributed 59.36%, mainly in the central and western regions. The remaining 40.64% was attributable to ET, concentrated in the northeast region. (5) Human activities significantly affected WUE, with a contribution (about 53.52%) larger than that of climate change (nearly 46.48%). Increased precipitation improves vegetation WUE and is the most important climate factor influencing WUE variations. These findings will aid the formulation of vegetation protection and water resource management strategies in water-stressed areas
Antidepressant Mechanism Research of Acupuncture: Insights from a Genome-Wide Transcriptome Analysis of Frontal Cortex in Rats with Chronic Restraint Stress
Major depressive disorder (MDD) is a chronic disease that adversely affects mood and cognition. In this study, we randomly divided the rats into control group (C), model group (M), fluoxetine group (F), and acupuncture group (A), used open-field test to ascertain whether acupuncture affects chronic restraint stress (CRS) induced depression-like behaviors of rats, and explored the antidepressant mechanism of acupuncture at the molecular level of transcriptome in the frontal cortex of CRS rats by RNA-sequencing (RNA-seq). According to differentially expressed genes (DEG) analysis, we identified 134, 46, and 89 response genes differentially expressed in C versus M, F versus M, and A versus M, respectively. Through Gene Ontology (GO) term enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis, we identified the gene sets involved in extracellular space, inflammatory response, Toll-like receptor signaling pathway, chemokine signaling pathway, and TNF signaling pathway. In this study, RNA-seq technology was used to investigate the frontal cortex genome-wide transcriptomes in depression rats under CRS, which suggested that the antidepressant effect of acupuncture is effective and has a multitarget characteristic, which may be related to amino acid metabolism and inflammatory pathways, especially the Toll-like receptor signaling pathway, TNF signaling pathway, and NF-kappa B signaling pathway