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Additional file 3: Figure S1. of Isolation, cultivation and molecular characterization of a new Trypanosoma equiperdum strain in Mongolia
The phylogenetic tree of the 18S rRNA and ITS region. A phylogenetic analysis was performed using the T. equiperdum IVM-t1, SITB818, STIB841, STIB842, BoTat1.1, T. evansi Tansui (Accession No. D89527.1), Cairo (AB551922.1), KAI.2 (AY912277), Sam.2 (AY912279.1), T. brucei TREU927 (AC012647), T. b. gambiense DAL972 (FN554966.1), T. b. gambiense Tsuua (AJ009141) and T. b. rhodesiense Utro (AJ009142) sequences. A: A phylogenetic tree based on the 18S rRNA sequence. B: A phylogenetic tree based on the ITS sequence. Figure S2. The maxicircle PCR of the Trypanozoon species. Gel electrophoresis images of the PCR products are shown in A to G, NADH-dehydrogenase subunit 7 (NAD7; 383Â bp), Cytochrome oxidase subunit 2 (Cox2; 1747Â bp), ATOas subunit 6 (A6; 299Â bp), 12S ribosomal RNA (12S rRNA; 1597Â bp in T. b. brucei GUTat3.1 strain and T. equiperdum STIB818 strain, 1415Â bp in T. equiperdum STIB841, STIB842, BoTat1.1 strains, respectively), NADH-dehydrogenase subunit 7-cytochromeB (ND7-CyB; 1450Â bp), Maxicircle unknown reading frame-NADH dehydrogenase subunit 1 (MURF-ND1; 1779Â bp) and Maxicircle unknown reading frame 2-cytochrome oxidase subunit 1 (MURF2-Cox1; 1551Â bp), respectively. M: the 100Â bp and 1 kbp DNA ladders; Lanes 1 to 8 show T. b. brucei GUTat3.1, T. evansi IL3960, T. equiperdum IVM-t1, STIB818, STIB841, STIB842, BoTat1.1 strains and negative control (distilled water), respectively. (PPTX 164 kb