The measurement of sediment irrigation rates: A comparison of the BR- tracer and 222RN/226RA disequilibrium techniques

We have carried out a series of experiments designed to allow comparison of sediment irrigation rates determined simultaneously using two methods: the measurement of 222Rn/226Ra disequilibrium in pore waters, and the measurement of distributions of a tracer, Br−, which was added to the water overlying sediments at the start of incubation experiments. The experiments were carried out on fine-grained sediments from Buzzards Bay, MA. We made irrigation rate measurements on sediments in their natural state, as well as on sediments that had been treated to alter macrofaunal abundance and diversity. The range of irrigation rates measured was similar for both tracers, and was similar to rates measured at the study site previously by Martin and Sayles (1987). Furthermore, the two tracers gave similar patterns of irrigation rate variability between cores and with depth below the sediment-water interface. On the other hand, comparisons of individual cores showed significant differences in the absolute rates measured using the different tracers; in particular, the 222Rn/226Ra disequilibrium method yielded more rapid irrigation rate estimates at depths exceeding 10 cm below the sediment-water interface. These differences could be due to the inherent limitations on the sensitivity of the methods, to artifacts in measurement procedures, to differences in the permeability of burrow walls to the two tracers (Rn and Br−), or to differences in the time-scales on which the two tracers record irrigation events. Irrigation rates determined by the Br− tracer method were roughly correlated with the abundance of Nephtys incisa in the sediments, but were not related to abundances of the other numerically important deposit feeders, Nucula annulata and Mediomastus ambiseta.

Are Nordic Saltmarshes Europe’s Way to ‘Live in Harmony with Nature’? Scientists Driven Future Scenarios via a Participatory Workshop

Saltmarshes have the ability to not only promote biodiversity, but to put nations on the path towards climate recovery and net-zero emissions through saltmarshes’ capability to take up carbon. As the European Union’s (EU) Green Deal sets out to reach net-zero emissions by 2050, innovative solutions will need to be identified, possibly even through better preserving century-old habitats such as saltmarshes. Based on the upcoming needs from the EU, in the Spring of 2021, a workshop was held with leading Nordic saltmarsh and blue carbon scientists using the transdisciplinary methods of Systems Thinking and Bayesian Belief Networks to identify solutions that can include saltmarshes in future policy. These joint methods elicited multiple future scenarios in which data were collected on perceived notions of the value of saltmarshes and how to better govern them to ensure their longevity. The models developed in this study include human perceptions and comprehensive quantitative scenarios through their ability to define paths forward in the form of comprehensive policy recommendations. We found through scenario analysis that a major belief among the stakeholders was numerous events of change such as ‘outreach, getting salt marshes on the political agenda and forming new narratives would help to increase saltmarsh area via conservation and restoration prioritization’ would have a positive impact of saltmarshes in Nordic countries.publishedVersio

PREPARATION OF INORGANIC CATALYST BASED HEMATITE (a-Fe2O3) COMPOUND EXTRACTED FROM ACEH IRON ORE MINERAL AND ITS CATALYTIC ACTIVITY ON TRANSESTERIFICATION OF COCONUT OIL

Hematite (Fe2O3) was successfully extracted from the Aceh iron ore mineral by precipitation method with employing sulphuricacid (H2SO4) as chemical agent.  Furthermore, the extracted hematite was modified with sodium metal (K), namely it doped with NaOH of 5% and 10% (w/w), respectively. Based on the characterization results using XRD and SEM- EDS proved that modified catalyst (Na2O/Fe2O3) with their homogeneous size were resulted while the hematite (Fe2O3) particles was dopped withNaOH at its low concentration of (5% wt/wt), however while doping with the higher concentration of NaOH (10% w/w), agglomeration was taken place among the catalyst particles in which decreasing the surface area of the modified catalysts. Finally, the modified catalyst (Na2O/Fe2O3) was successfully applied as an inorganic catalyst on transesterification of coconut oil and methanol (T = 55 – 60oC, 2 h)for forming methyl esters asbiodiesel compounds.

A challenging diagnosis of alpha-1-antitrypsin deficiency: identification of a patient with a novel F/Null phenotype

Alpha-1-antitrypsin (A1AT) deficiency is a genetic disease characterized by low levels and/or function of A1AT protein. A1AT deficiency can result in the development of COPD, liver disease, and certain skin conditions. The disease can be diagnosed by demonstrating a low level of A1AT protein and genotype screening for S and Z mutations, which are the most common. However, there are many genetic variants in A1AT deficiency, and this screening may miss rarer cases, such as those caused by dysfunctional protein. We identified a patient with a previously unreported F/null phenotype that was missed by routine screening. This case highlights the wide variation in possible mutations, limitations in diagnostics, and the importance of combining clinical suspicion with measurement of protein levels, phenotypic analysis, and in appropriate cases expanded genetic analysis

Potential Distribution of Six North American Higher-Attine Fungus-Farming Ant (Hymenoptera: Formicidae) Species

Ants are among the most successful insects in Earth’s evolutionary history. However, there is a lack of knowledge regarding range-limiting factors that may influence their distribution. The goal of this study was to describe the environmental factors (climate and soil types) that likely impact the ranges of five out of the eight most abundant Trachymyrmex species and the most abundant Mycetomoellerius species in the United States. Important environmental factors may allow us to better understand each species’ evolutionary history. We generated habitat suitability maps using MaxEnt for each species and identified associated most important environmental variables. We quantified niche overlap between species and evaluated possible congruence in species distribution. In all but one model, climate variables were more important than soil variables. The distribution of M. turrifex (Wheeler, W.M., 1903) was predicted by temperature, specifically annual mean temperature (BIO1), T. arizonensis (Wheeler, W.M., 1907), T. carinatus, and T. smithi Buren, 1944 were predicted by precipitation seasonality (BIO15), T. septentrionalis (McCook, 1881) were predicted by precipitation of coldest quarter (BIO19), and T. desertorum (Wheeler, W.M., 1911) was predicted by annual flood frequency. Out of 15 possible pair-wise comparisons between each species’ distributions, only one was statistically indistinguishable (T. desertorum vs T. septentrionalis). All other species distribution comparisons show significant differences between species. These models support the hypothesis that climate is a limiting factor in each species distribution and that these species have adapted to temperatures and water availability differently

Recent Cases

Constitutional Law-First Amendment-School Authorities May Prohibit High School Student\u27s Distribution of Sex Questionnaire to Prevent Possible Psychological Harm to Other Students Robert Edward Banta Plaintiff, editor of a high school publication,\u27 brought suit in federal court seeking an order compelling defendant school officials to allow the student publication to distribute a sex questionnaire,to students in the high school and to publish the results. Plaintiff claimed that defendants had not shown that the planned distribution would disrupt school activities and that, therefore, defendants\u27prohibition of the questionnaire violated 42 U.S.C. § 19831 and the first and fourteenth amendments. Pointing to potential psychological harm to students, defendants argued that the state\u27s interest in protecting the students\u27 emotional well-being outweighed plaintiffs interest in distributing the questionnaire. The trial court held that defendants could prohibit distribution of the questionnaire to ninth-and tenth-grade students but not to eleventh- and twelfth-grade students. On appeal to the United States Court of Appeals for the Second Circuit, held, reversed in part\u27 and remanded with instructions to dismiss the complaint. If school officials reasonably believe that distribution on school grounds of a high school student\u27s questionnaire soliciting information about the sexual habits of his fellow students might cause psychological harm to other students, then prohibition of the questionnaire does not violate the right to freedom of expression of the student seeking to distribute the questionnaire. Trachtman v. Anker, 563 F.2d 512 (2d Cir. 1977). Corporations--Freeze-Out Mergers--The Delaware Supreme Court Requires Majority Shareholder Proof of a Valid Business Purpose As a Component of Entire Fairness in Freeze-Out Merger Challenges Oby T. Brewer, III Recent declines in stock market averages\u27 accompanied by costly disclosure requirements imposed under the federal securities laws have prompted many companies to reconsider their positions as publicly held corporations. In response to the resulting minimal benefits of public ownership, many controlling shareholders now seek to increase their control and participation in a corporation\u27s future profits by going private.\u27 One means of going private is the freeze-out merger, by which a parent company forces the liquidation of minority interests in a publicly held subsidiary through a merger of the subsidiary with the parent. By complying with applicable state merger statutes, the parent may eliminate the minority\u27s shares by tendering a cash-out price, which the minority either must accept or have appraised judicially. The merger statutes thus represent a legislative compromise between total majority control and the single vote veto available to the minority at common law. Criminal Procedure--Prosecutorial Immunity-Federal Prosecutor Is Not Absolutely Immune From Suit for Alleged Perjury Cornelia Anne Clark Plaintiffs\u27 brought a civil action in tort charging that defendant federal prosecutor\u27s alleged perjury at a hearing incident to a grand jury investigation of plaintiffs\u27 activities\u27 violated their constitutional rights. On motion to dismiss, defendant contended that he enjoyed absolute quasi-judicial immunity because he had been acting at the hearing in his official capacity as a special federal prosecutor. The district court\u27 denied the motion, holding that the doctrine of quasi-judicial immunity does not apply when the prosecutor is alleged to have committed perjury. On interlocutory appeal to the United States Court of Appeals for the District of Columbia Circuit, held, affirmed. Because a federal prosecutor\u27s perjury during a court hearing incident and prior to a grand jury investigation falls within his investigative duty rather than his advocatory duty,the prosecutor is entitled only to a qualified immunity.\u27 Briggs v.Goodwin, No. 75-1642 (D.C. Cir. Sept. 21, 1977). Securities Law--Rule 10b-5-Defense of In Pari Delicto Bars Private Damage Action Brought Against Tipper by Tippee Who Fails to Disclose Before Trading Terry Currie Tippees\u27 brought a private action for damages under section 10(b) of the Securities Exchange Act of 1934 and rule 10b-53 charging that defendant tippers disseminated false and misleading material inside information in advising plaintiffs of an imminent merger between two corporations that would result in appreciated stock values.\u27 In reliance on this information, plaintiffs purchased stock in one of the corporations and subsequently incurred substantial losses on the stock when the proposed merger did not occur. Defendants moved for summary judgment, claiming that the doctrine of in pari delicto barred plaintiffs\u27 recovery because plaintiffs also had violated rule 10b-5 by failing to make full disclosure of the inside information before trading on the open market.\u27 The district court granted the tippers\u27 motion for summary judgment. On appeal to the United States Court of Appeals for the Third Circuit, held, affirmed. When a tippee violates rule 10b-5 by failing to make full disclosure to the investing public of material inside information prior to trading on the open market, the defense of in pari delicto bars a rule 10b-5 private damage action by the tippee against the tipper. Torts--Negligence--Child Has Cause of Action for Preconception Medical Malpractice Douglas William Ey, Jr. Plaintiff, claiming that she suffered permanent physical injuries\u27 as a result of defendants\u27 conduct prior to her conception,sought to recover damages in a negligence action.2 In 1965 plaintiffs mother,who had Rh negative blood, was given two transfusions of incompatible Rh positive blood in defendant hospital where defendant physician was director of laboratories.\u27 Plaintiff\u27s mother did not discover that these transfusions had sensitized her blood until shortly before plaintiffs birth in 1973. Defendants moved to dis-miss for failure to state a cause of action, arguing that because plaintiff had not been conceived at the time of the alleged negligent conduct, defendants owed no duty of care to plaintiff. The trial court granted defendants\u27 motion, but the Illinois Appellate Court reversed and remanded the case for further proceedings. On appeal to the Supreme Court of Illinois, held, affirmed. A child injured by the negligent acts of a physician and a hospital committed against his mother prior to his conception has a cause of action based on negligence. Renslow v. Mennonite Hospital, 367 N.E.2d 1250 (Ill.1977)

Elevated acetate concentrations in the rhizosphere of Spartina alterniflora and potential influences on sulfate reduction

Acetate is important in anaerobic metabolism of non-vegetated sediments but its role in salt marsh soils was not investigated thoroughly. Acetate concentrations, oxidation (C-14) and SO4(2-) reduction (S-35) were measured in S. alterniflora soils in NH and MA. Pore water from cores contained greater than 0.1 mM acetate and in some instances greater than 1.0 mM. Non-destructive samples contained less than 0.01 mM. Acetate was associated with roots and concentrations were highest during vegetative growth and varied with changes in plant physiology. Acetate turnover was very low whether whole core or slurry incubations were used. Radiotracers injected directly into soils yielded rates of SO4(2-) reduction and acetate oxidation not significantly different from core incubation techniques. Regardless of incubation method, acetate oxidation did not account for a significant percentage of SO4(2-) reduction. These results differ markedly from data for non-vegetated coastal sediments where acetate levels are low, oxidation rate constants are high and acetate oxidation rates greatly exceed rates of SO4(2-) reduction. The discrepancy between rates of acetate oxidation and SO4(2-) reduction in marsh soils may be due either to the utilization of substrates other than acetate by SO4(2-) reducers or artifacts associated with measurements of organic utilization by rhizosphere bacteria

Capturing of organic carbon and nitrogen in eelgrass sediments of southern Scandinavia

The ability of seagrass meadows to filter nutrients and capture and store CO2 and nutrients in the form of organic carbon (OC) and nitrogen (N) in their sediments may help to mitigate local eutrophication as well as climate change via meadow restoration and protection. This study assesses OC and N sediment stocks (top 50 cm) and sequestration rates within Danish eelgrass meadows. At four locations, eelgrass-vegetated and nearby unvegetated plots were studied in protected and exposed areas. The average OC and N sediment 50 cm stocks were 2.6 ± 0.3 kg OC m − 2 and 0.23 ± 0.01 kg N m − 2, including vegetated and unvegetated plots. In general, OC and N stocks did not differ significantly between eelgrass meadows and unvegetated sediments. Lack of accumulation of excess 210Pb suggested sediment erosion or low rates of sediment accumulation at most sites. OC accumulation rates ranged from 6 to 134 g m − 2 yr − 1 and N from 0.7 to 14 g m − 2 yr − 1. Generalized additive models showed that ≥ 80 % of the variation in sediment OC and N stocks was explained by sediment grain size, organic matter source, and hydrodynamic exposure. Long cores, dated with 210Pb, showed declining OC and N densities toward present time, suggesting long-term declines in eelgrass OC and N pools. Estimates of potential nation-wide OC and N accumulation in eelgrass sediments show that they could annually capture up to 0.7 % ± 0.5 % of CO2 emissions and 6.9 % ± 5.2 % of the total terrestrial N load

Using Comparative Genomics for Inquiry-Based Learning to Dissect Virulence of Escherichia coli O157:H7 and Yersinia pestis

Genomics and bioinformatics are topics of increasing interest in undergraduate biological science curricula. Many existing exercises focus on gene annotation and analysis of a single genome. In this paper, we present two educational modules designed to enable students to learn and apply fundamental concepts in comparative genomics using examples related to bacterial pathogenesis. Students first examine alignments of genomes of Escherichia coli O157:H7 strains isolated from three food-poisoning outbreaks using the multiple-genome alignment tool Mauve. Students investigate conservation of virulence factors using the Mauve viewer and by browsing annotations available at the A Systematic Annotation Package for Community Analysis of Genomes database. In the second module, students use an alignment of five Yersinia pestis genomes to analyze single-nucleotide polymorphisms of three genes to classify strains into biovar groups. Students are then given sequences of bacterial DNA amplified from the teeth of corpses from the first and second pandemics of the bubonic plague and asked to classify these new samples. Learning-assessment results reveal student improvement in self-efficacy and content knowledge, as well as students’ ability to use BLAST to identify genomic islands and conduct analyses of virulence factors from E. coli O157:H7 or Y. pestis. Each of these educational modules offers educators new ready-to-implement resources for integrating comparative genomic topics into their curricula