Analytical method development and validation of assay test of pravastatin sodium tablets

A simple, accurate, precise and stability indicating Ultra performance liquid chromatographic method for determination of pravastatin sodium in tablet dosage form. The separation was carried on Acquity UPLC ® HSS C18, 2.1 × 100mm, 1.8µm ID column, with mobile phase comprising of mixture of pH 5.5 buffer: methanol in the ratio of 30 : 70 v/v, as the mobile phase at a flow rate  0.2 ml/min and the detection was carried out using UV-visible detector at 238nm. The method was validated by evaluation of different parameters such as accuracy, precision, linearity, ruggedness, robustness, filter equivalency, solution stability. The retention time were found to be 1.5 min. Calibration curves were linear with correlation coefficient (r2) 0.999. The Percent assay of Pravastatin sodium tablet was found to be 98.4%. The developed methods were validated as per the ICH guidelines. Keywords: Pravastatin sodium (PVS), UPLC, Method Validation

Formulation, development and evaluation of Microsponge loaded Topical Gel of Nystatin

Nystatin containing microsponge as active constituent (API) in different formulations by changing the proportions of drug (Nystatin), polymer (ethyl cellulose), emulsifier (Poly vinyl alcohol) were obtained successfully using quasi-emulsion solvent diffusion method. These formulations were studied for particle size and physical characterization. Scanning electron microscopy (SEM) images showed the microsponges porous and spherical in shape. The physical characterization showed that microsponge formulation coded by P6 showed a better loading efficiency and production yield. This microsponge formulation was prepared as gel in carbopol and studied for pH, viscosity, spreadability, drug content, in-vitro release. The microsponge formulation gel, F3 showed viscosity3465.84cps,spreadability of 26.22g cm/s and drug content of 89.65%. The nystatin microsponge gel formulations showed an appropriate drug release profile. F3 released 81.03% of drug at 12 hours. Keywords: Microsponge, Solvent diffusion method, Scanning electron microscope, Nystatin microsponge gel