3 research outputs found
Advances and challenges in adeno-associated viral inner-ear gene therapy for sensorineural hearing loss
There is growing attention and effort focused on treating the root cause of sensorineural hearing loss rather than managing associated secondary characteristic features. With recent substantial advances in understanding sensorineural hearing-loss mechanisms, gene delivery has emerged as a promising strategy for the biological treatment of hearing loss associated with genetic dysfunction. There are several successful and promising proof-of-principle examples of transgene deliveries in animal models; however, there remains substantial further progress to be made in these avenues before realizing their clinical application in humans. Herein, we review different aspects of development, ongoing preclinical studies, and challenges to the clinical transition of transgene delivery of the inner ear toward the restoration of lost auditory and vestibular function
Oleoyl-Chitosan-Based Nanofiber Mats Impregnated with Amniotic Membrane Derived Stem Cells for Accelerated Full-Thickness Excisional Wound Healing
Wound
healing management is a major challenge for critical full-thickness
skin wounds. Development of nanofibrous scaffolds with tunable wettability,
degradation, and biocompatibility are highly desirable. Herein, we
demonstrated synthesis of oleoyl chitosan (OC) by grafting monounsaturated
fatty acid residue, C<sub>18</sub> oleoyl chain, to the backbone of
chitosan molecule and blending with gelatin to form the nanofiber
mats. The physicochemical properties of the nanofiber mats revealed
mechanical strength, moderate surface wettability, and suitable degradation
rate. The nanofibrous mats showed excellent in vitro cytocompatibility
with human amniotic membrane-derived stem cells (HAMSCs) in terms
of enhanced adhesion and proliferation owing to biomimetic nanoarchitecture
and chemical cues. Furthermore, the fabricated nanofiber was implanted
with and without preseeded HAMSCs in the full-thickness wound to evaluate
the skin wound healing efficacy in a rat model. Histological and immunohistochemical
studies were conducted to evaluate the plausible changes of tissue
architecture and expression of molecular markers involved in wound
healing process. Both acellular and HAMSCs incorporated cellular nanofibers
promoted wound contraction remarkably with superior skin tissue regeneration
in terms of enhanced collagen synthesis, re-epithelialization and
initiation of epithelial cells stratification compared to control
group