9 research outputs found
Diagnostic graphics produced for synergistic effect quantification of DOX/Dis/Hyd combination against MCF-7_DoxR cells.
(A) Dose -Fa curve for DOX alone and for DOX with (DOX/Dis/Hyd combination) at different concentration points (B) Table of IC50 of DOX, Dis, Hyd alone and combination (C) The fraction affected (Fa) versus combination index (CI) plot after treatment with DOX/Dis/Hyd combination, that most of CI values are < 1 for the range of 0.25–0.8 (D) The Fa-DRI plot for the non-constant ratios of DOX/Dis/Hyd combination.</p
Fig 7 -
IC50 values after treatment with DOX/Dis/Hid. The MCF-7_WT and MCF-7_DoxR cells were treated with DOX, Dis, Hyd and DOX/Dis/Hyd to assess the cytotoxicity levels (A) The dose-response curve for cells treated with Hyd and; (B) The dose-response curve for cells treated with Dis; (C)The dose-response curve for cells treated with DOX; (D) The dose-response curve for cells treated with DOX/Dis/Hyd in combination, the curve showing DOX IC50 with and without combination with Dis/Hyd (0.03/20 μM). All cytotoxicity values represent the average ± SD of three independent experiments.</p
STRING analysis of protein–protein interactions.
(A) Interaction between proteins involved in drug resistance STAT3, NOTCH, DNMT, (B) Illustration of combination of HDACi and DNMTi pathway, (C) combination of HDACi and DNMTi and the effect of cell apoptosis proteins, (D) interactions of DNMTi and proteins ivoved in cell cycle, (E) Effect of ALDH enzymes on P53 proteins, (F) Interactions between ALDH and glutathione (GSH), (G) Interactions between ALDH, ABCC1 and p53 proteins, (H) Signalling pathway showed interaction of DNMT, ALDH, P53, TOP2A, ABCB1. STAT3, NOCH1. (thickness of edges indicate confidence).</p
Diagnostic graphics produced for synergistic affect quantification.
(A) Isobologram for Dis/Hyd combination at different concentration points, (B) The Fa-DRI plot (Chou-Martin plot) for the non-constant ratios of Dis/Hyd combination, (C) The fraction affected (Fa) versus combination index (CI) plot after treatment with Dis/Hyd combination, that most of CI values are 50 of Dis, Hyd and Dis/Hyd combination.</p
Diagnostic graphics produced for synergistic effect quantification of DOX/Dis/Hyd combination against MCF-7_WT cells.
(A) Dose -Fa curve for DOX alone and for DOX with (DOX/Dis/Hyd combination) at different concentration points (B) Table of IC50 of DOX, Dis, Hyd alone and combination (C) The fraction affected (Fa) versus combination index (CI) plot after treatment with DOX/Dis/Hyd combination, that most of CI values are <1 for the range of 0.5–0.95(D) The Fa-DRI plot for the non-constant ratios of DOX/Dis/Hyd combination.</p
Fig 4 -
A) Induced apoptosis of MCF-7_WT cells treated with Dis and Hyd alone and combined B) Induced apoptosis of MCF-7_DoxR cells treated with Dis and Hyd alone and combined.</p
Apoptosis Induced by DOX/Dis/Hyd single treatment and combinations.
A) and B) MCF-7_WT cells, C) and D) MCF-7_DoxR cells.</p