In vitro comparison of the effect of honey and clotrimazole against Candida albicans isolated from vagina

Background and aims: Candida albicans is the most common cause of Vulvovaginitis Candidiasis that is the most common vaginitis in human. It is estimated that more than 90 of these infections are caused by Candida albicans. Medical treatment for these infections is carried out with chemotherapeutic drugs such as azoles. The increasing resistance of C. albicans to the azoles as well as their probable side effects is public concerns. Therefore, research for new natural component having antifungal activity has been considered to be very important. This study was designed to compare the effect of honey and clotrimazole against Candida albicans isolated from vagina and standard strain. Methods: In this clinical trial study, Samples of vaginal discharges were prepared from 100 women with vulvovaginitis candidiasis. Isolation and complete identification of Candida species were performed andthe suspension of Candida albicans prepared for insemination. Different concentrations of honey and clotrimazol were prepared and 10 µl of yeast suspension was added and incubated. Then 10 µl of these medium was cultured. Finally, the number of yeasts was counted and MIC50 and MIC90 were determined in comparison with positive control. Results: Different concentrations of honey and clotrimazol inhibited the growth of Candida albicans. In MIC 50 the mean concentrations of honey and clotrimazol were 1.15±0.49 g/ml and 60.26± 6.24 µ g/ml respectively. In MIC 90 the mean concentrations of honey and clotrimazol were 3.10± 3.15 g/ml and 2.38±2.36µ g/ml respectively. This means that honey in 3.10 g/ml concentration had the same effect as clotrimazol with concentration of 2.38µ g/ml. Conclusion: Honey markedly inhibited the growth of Candida albicans and its effect was comparable to cloitrimazol

Comparing the effects of massage and intra intramuscular pethidine on pain relief in the stages of labor and the length of labor

Introduction: Systemic opioids and massage therapy are widely used for pain relief during labor. The aim of this study was to compare between massage and intramuscular pethidine (meperidine) for pain relief in the stages of labor and the length of labor. Methods: This clinical trial was conducted on 120 pregnant women referred to valiasr hospital in Broojen, Iran in 2012. Subjects were randomly allocated into three groups of massage, intramuscular pethidine and standard care. Data were collected using interview forms, observation and examination. Pain intensity was measured using visual analogue scale. Data were analyzed using SPSS software version 20 and descriptive statistics, paired t-test, chi-square, ANOVA, Spearman and Pearson correlation coefficient tests. P value less than 0.05 was considered significant. Results: Pain intensity showed significant differences between the three groups, 30 minutes after the intervention and pain intensity was lower in the massage group than other groups (p=0.001). Intensity of uterine contractions showed significant differences between the three groups and it was lower in massage group than other groups (p<0.0001). There was no significant differences in duration of the first (p=0.086) and the second stages (p=0.295) of labor in all groups. Conclusion: Massage and intramuscular pethidine reduced pain during labor, but massage therapy provided more persistent pain relief and without any side effects. Massage therapy is a safe, effective and inexpensive intervention and it can be used for pain relief during labor

Comparison of vaginal ointment of honey and clotrimazole for treatment of vulvovaginal candidiasis: A random clinical trial

BACKGROUND AND OBJECTIVE: Vulvovaginal candidiasis (VVC) is the most prevalent vaginitis in women, accounting for 10 million medical referrals a year. Vaginal clotrimazole is a drug of choice for VVC treatment. However, increased drug resistance to this microorganism has led to an interest in naturally derived antifungal drugs. This study was conducted to compare honey vaginal ointment and clotrimazole vaginal ointment for VVC treatment. METHODS: Eighty women diagnosed with VVC were assigned to two groups for honey ointment and clotrimazole ointment treatment using a simple randomization rule. The ointments were applied at night for seven days. The disease symptoms including inflammation, vaginal discharge, and irritation at baseline in the fourth and eighth days of treatment were examined and compared between the two groups. The data was analyzed by SPSS version 20 with the Friedman test, Chi-square test, and independent t-test. P<0.05 was considered as the significance. RESULTS: The two groups were similar for inflammation severity, irritation, and discharge at baseline. In both the groups, the symptoms disappeared after treatment. On the eighth day of treatment, there was a significant difference in inflammation and vaginal discharge between the two groups. Inflammation (P=0.002) and vaginal discharge (P=0.003) recovered better in the clotrimazole group. But there was no significant difference in irritation severity and satisfaction with treatment between the two groups. In the two groups, no side effects were reported. CONCLUSION: Honey contributes to treating VVC. Thanks to the popular positive attitudes of honey, its availability, no need for sterility, and its cost-effectiveness, it is a choice of treatment for VVC. Copyright © 2017. Published by Elsevier Masson SAS

A COMPARATIVE STUDY OF THE EFFECTS OF CLOTRIMAZOLE AND SALVIA OFFICINALIS EXTRACT AGAINST VAGINAL CANDIDA ALBICANS ISOLATED FRO...

Background: Vaginal Candidiasis is the most common vaginitis in women and Clotrimazole is the choice treatment. The side effects of drugs and microorganisms resistant are big problems in medicine and herbal therapy seems to be suitable alternative. This study was carried out to evaluate the antifungal effects of Clotrimazole and Salvia officinalis on vaginal C. albicans. Materials and Methods: A vaginal sample of 100 women with vaginal candidiasis was collected in the tube containing 1cc of normal saline. These samples

In vitro effect of honey on Candida albicans and lactobacillus

زمینه و هدف: یکی از شایع ترین واژینیت ها در زنان واژینیت های کاندیدایی است که عامل آن قارچ کاندیدا آلبیکنس می باشد. لاکتوباسیلوس از شایع ترین فلورهای طبیعی واژن می باشد که در حفظ شرایط میکروبیولوژی واژن اهمیت زیادی دارد. این مطالعه با هدف بررسی آزمایشگاهی تاثیر عسل بر کاندیدا آلبیکنس و لاکتوباسیلوس انسانی انجام شده است. روش بررسی: در این مطالعه تجربی آزمایشگاهی غلظت های مختلف (وزنی حجمی) بین 20 تا 95 درصد عسل تهیه شد. پس از کشت کاندیدا در محیط کشت RPMI 1640 و عسل و لاکتوباسیلوس در محیط کشت تریپتی کیس سوی براث (TBS) و عسل و انکوباسیون، میزان رشد این میکروارگانسیم ها در ساعات مختلف مورد برسی قرار گرفت. یافته ها: ازغلظت های مورد بررسی، غلظت80 عسل به میزان زیادی از رشد کاندیدا جلوگیری نمود که بیشترین تاثیر مهار کنندگی عسل بر کاندیدا را نشان داد. در محیط های حاوی عسل با غلظت های کمتر و بیشتر از 80 میزان رشد کاندیدا متوسط یا شدید بود. در محیط کشت شاهد، قارچ به طور کامل رشد نمود. هیچکدام از غلظت های عسل نتوانست رشد لاکتوباسیلوس را مهار نماید. نتیجه گیری: با توجه به نتایج بدست آمده عسل می تواند به طور قابل توجهی از رشد کاندیدا جلوگیری کند بدون اینکه تاثیری بر روی لاکتوباسیلوس داشته باشد. با توجه به اینکه لاکتوباسیلوس یک فلور طبیعی واژن است، این مسئله حائز اهمیت است که عسل بدون تغییر فلور طبیعی واژن می تواند رشد کاندیدا را مهار نماید

Comparison of the effect of honey and miconazole against Candida albicans in vitro.

BACKGROUND One of the most common causes of vaginitis is candidiasis. The aim of this study is to compare the effect of honey and miconazole against Candida albicans, in vitro. MATERIALS AND METHODS The different W/V concentrations of honey were prepared at 20, 40, 60, 80, and 95% and different dilutions of miconazole were prepared in 0.05, 5, and 50 μg/ml. A microdilution of 100/000 cells per ml of a two-day old culture of Candida albicans was prepared in normal saline, after culturing the strain of PTCC 5027 in RPMI 1640 medium. Ten microliters of this dilution was added to 1 ml of the RPMI 1640 medium containing different concentrations of honey and to 1 ml of the RPMI 1640 medium containing different dilutions of miconazole. The cultures were incubated at 35°C for 12, 24, and 48 hours. RESULTS The growth rate of Candida albicans was determined in the cultures. The results indicated that the honey prevented the growth of C. albicans greatly only at an 80% concentration, whereas, miconazole inhibited it completely. CONCLUSIONS As Candida albicans is a normal vaginal flora, the inhibitory effect of honey without the fungicide effect is a very good trend in the treatment of vaginal candidiasis

A comparative study of the effects of Clotrimazole and Salvia officinalis extract against vaginal Candida albicans isolated from women with candidacies vaginit

Background: Vaginal Candidiasis is the most common vaginitis in women and Clotrimazole is the choice treatment. The side effects of drugs and microorganisms resistant are big problems in medicine and herbal therapy seems to be suitable alternative. This study was carried out to evaluate the antifungal effects of Clotrimazole and Salvia officinalis on vaginal C. albicans. Materials and Methods: A vaginal sample of 100 women with vaginal candidiasis was collected in the tube containing 1cc of normal saline. These samples ..

Midwifery Students’ Self Assessment of their Ability in Practical Skills

Aims: Use of senior students’ viewpoints about the learned skills during education , can help to reinforcment the strenght and eliminating the weakness points of education. The current study was carried out with the purpose of evaluating the self-assessment of senior midwifery students of Shahrekord University of Medical Sciences from their abilities in practical skills . Methods: This cross-sectional study was carried out in undergraduate midwifery students who were ready for the final exam in second semester of 2009-10 and second semester of 2010-11 academic years in Shahrekord University of Medical Sciences and all of 47 students were studied. Data were collected by practical skills assessment form in areas of gynecology, obstetrics, maternal and child health, infants and fundamentals of nursing and midwifery. SPSS 16 software and descriptive statistics were used to analyse the data. Results: 78.3% of students were satisfied from midwifery group, 76.1% from Shahrekord University of Medical Sciences and 82.6% from midwifery field. The assessment of 61% of students was high of their abilities in obstetrics area , 60% in gynecologic area , 54% in fundamentals of nursing and midwifery area, 41% in maternal and child health area and 33% in infants area. The midwifery students considered themselves essentially unskilled in doing of breech delivery, multiple delivery and inserting the intra uterine device (IUD). Conclusion: The midwifery students assessed their skills as moderate in areas of obstetrics, gynecology and fundamentals of nursing and midwifery and Less than moderate in areas of maternal and child health and infants