NIRS International Symposium on Visualization of Radiation Response at the Molecular and Cellular Levels

細胞・分子イメージング研究の近年の発展を背景にして、国際・交流課と重粒子医科学センター粒子線生物研究グループとの共催で、３月12-13日に「細胞および分子レベルでの放射線応答の可視化」(Visualization of Radiation Response at the Molecular and Cellular Levels)と題する放医研国際シンポジウムを開催した

バイヨウサレタヒトニバイタイサイボウニオヨボスデンリホウシャセンタンイツショウシャノキュウセイコウカトバンハツコウカ

京都大学0048新制・課程博士医学博士甲第2311号医博第542号新制||医||269(附属図書館)6099UT51-55-G6京都大学大学院医学研究科生理系専攻(主査)教授 阿部 光幸, 教授 鳥塚 莞爾, 教授 菅原 努学位規則第5条第1項該当Kyoto UniversityDA

Dose Thioredoxin-1 Prevent Mitochondria- and Endoplasmic Reticulum-Mediated Neurotoxicity of 1-Methyl-4-phenyl-1,2,3,6-Tetrahydropyridine?

We show that 1-methyl-4-phenylpyridinium ion(MPP+), and active metabolite of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP),induces cytotoxicity via endoplasmic reticulum(ER)-and mitochondria-mediated pathways, and thioredoxin-1(TRX-1), a redox-active protein, prevents MPTP-induced neurotoxicity

gammaH2AX foci formation in x-irradiated mammalian cell lines with different radio-sensitivities

DNA double-strand breaks (DSBs) induced by ionizing radiation represent the most serious damage in cells. Immediately after the formation of DSB, Ser-139 of histone H2AX is phosphorylated at the site of DSB. The phosphorylated H2AX (gammaH2AX) foci can be observed with an immunohistochemical staining using anti-gammaH2AX antibody. It is also known that each gammaH2AX focus corresponds to one DSB and disappears when the DSB is rejoined. Using this method, we compared the amount of unrejoined DSBs among cell lines with different radiation sensitivities after X irradiation. At 30 min following 2 Gy of X-rays, the number of foci observed is similar in all cell lines studied. However, the disappearance of foci in radioresiatant colon cancer cell lines (SW-480 and CaCo-2) was faster than that in radiosensitive ones (SW-48 and LoVo). At 12 hr after X-irradiation, few foci were observed in SW-480 and CaCo-2 cells, while many foci were remained in SW-48 and LoVo cells. Furthermore, the number of focus disappeared much slower in irradiated radiosensitive AT and 180BR cells as compared to normal human fibroblasts. Similar results were obtained when cells from radiosensitive LEC rat were compared with cells from Fisher rat. Our data demonstrate that the rejoining kinetics of DSBs measured by gammaH2AX foci formation correlates with the cellular radiosensitivities.The 12th International Congress of Radiation Research(ICRR