A multi-stages multi-objective assignment for facilities layout design

Al-Ma'amun factory of the General Company for Food Products suffers from inefficient facilities layout of its production departments and warehouses of both types (warehouses of raw materials and warehouses of finished products), which causes a lot of waste of time, resources and effort in the process of transporting raw materials or even individuals, which is leads to an increase in the costs of handling materials and not providing the service in time. Therefore, this study came to solve the problem by constructing a mathematical model based on the method of multi-stage assignment to find the optimal assignment, since the management of the factory has many goals, it was necessary to use an efficient mathematical method which is goal programming. Therefore, a multi-stage multi-objectives assignment method is applied in two stages, the first of which includes the process of transporting raw materials from raw material warehouses to the production departments to carry out the manufacturing process within them, the second stage includes the transfer of finished products from the production department to the final production warehouses. After comparing the results of the proposed layout with the results of the current layout of the factory is achieved an optimum layout of the factory because of it reduced the total traveled distance by (%25) per day, it also reduced the overall time spent by (25.5%) and reduced the total volume of spent fuel by (30.7%) per day for the new layout

Bronchial wash miR-21 as a potential biomarker for non-small cell lung cancer

Background: Lung cancer is one of the major health problems all over the world. Most of cases are discovered at advanced stages because of late appearance of symptoms and the lack of efficient and effective methods for early diagnosis and screening of high risk groups. The microRNA-21(miR-21) was stably present and reliably measurable in all samples of bronchial wash whether positive or negative (control) for lung cancer. Objectives: to evaluate the expressions of the miRNA-21 as a minimally invasive diagnostic biomarker for non-small cell lung cancer (NSCLC). Results: Relative quantification of miR-21 gene showed overexpression in samples positive for NSCLC (non-small cell lung cancer) and ROC study yielding 85% sensitivity and 98% specificity in distinguishing NSCLC patients from controls with p-value < 0.05. Conclusion: altered expressions of the miR-21 in samples of bronchial wash may provide a potential biomarker for detection of non-small cell lung cancer

Molecular Diagnosis of CoccidioidesPosadasii from Aborted Placenta of Ewes by RT-PCR

Diagnosis of diseases depends on histology using formaldehyde-fixed paraffin embedded tissue .The polymerase chain reaction (PCR)helps the pathologists to confirm the diagnosis of many disease including mycotic infections.Aborted placentas from 40 ewes were formalin fixed, paraffin embedded and sectioned. Tissue sections were stained with haematoxylin and eosine, and periodic acid Schiff (PAS) for histopathology examination. Fresh tissue parts were cultivated ,subjected to DNA extraction for the purpose of real-time PCR amplification.Histopathological examination of placental tissue revealed extensive necrotizing placentitis with the presence of spherule of CoccidioidesPosadasiiin the necrotic area. Only eight samples gave positive growth for coccidioidesPosadasii out of forty  aborted placental samples.DNA amplification by real-time PCR revealed positive amplification for the eight samples which confirmed the detection of coccidiodesposadasii

R229Q Polymorphism of NPHS2 Gene in Group of Iraqi Children with Steroid-Resistant Nephrotic Syndrome

Background. The polymorphism R229Q is one of the most commonly reported podocin sequence variations among steroid-resistant nephrotic syndromes (SRNS). Aim of the Study. We investigated the frequency and risk of this polymorphism among a group of Iraqi children with SRNS and steroid-sensitive nephrotic syndrome (SSNS). Patients and Methods. A prospective case control study which was conducted in Al-Imamein Al-Kadhimein Medical City, spanning the period from the 1st of April 2015 to 30th of November 2015. Study sample consisted of 54 children having NS, divided into 2 groups: patients group consisted of 27 children with SRNS, and control group involved 27 children with SSNS. Both were screened by real time polymerase chain reaction for R229Q in exon 5 of NPHS2 gene. Results. Molecular study showed R229Q polymorphism in 96.3% of SRNS and 100% of SSNS. There were no phenotypic or histologic characteristics of patients bearing homozygous R229Q polymorphism and the patients with heterozygous R229Q polymorphism. Conclusion. Polymorphism R229Q of NPHS2 gene is prevalent in Iraqi children with SRNS and SSNS. Further study needs to be done, for other exons and polymorphism of NPHS2 gene in those patients

R229Q Polymorphism of NPHS2 Gene in Group of Iraqi Children with Steroid-Resistant Nephrotic Syndrome

Background. The polymorphism R229Q is one of the most commonly reported podocin sequence variations among steroid-resistant nephrotic syndromes (SRNS). Aim of the Study. We investigated the frequency and risk of this polymorphism among a group of Iraqi children with SRNS and steroid-sensitive nephrotic syndrome (SSNS). Patients and Methods. A prospective case control study which was conducted in Al-Imamein Al-Kadhimein Medical City, spanning the period from the 1st of April 2015 to 30th of November 2015. Study sample consisted of 54 children having NS, divided into 2 groups: patients group consisted of 27 children with SRNS, and control group involved 27 children with SSNS. Both were screened by real time polymerase chain reaction for R229Q in exon 5 of NPHS2 gene. Results. Molecular study showed R229Q polymorphism in 96.3% of SRNS and 100% of SSNS. There were no phenotypic or histologic characteristics of patients bearing homozygous R229Q polymorphism and the patients with heterozygous R229Q polymorphism. Conclusion. Polymorphism R229Q of NPHS2 gene is prevalent in Iraqi children with SRNS and SSNS. Further study needs to be done, for other exons and polymorphism of NPHS2 gene in those patients