52 research outputs found
Effect of Intraoperative Hypotension on Survival After Resection of Colorectal Liver Metastases
Comparative analysis of preparation of human hepatocytes by the ethylenediamine tetraacetic acid and collagenase technique
Solvent-selective routing for centrifugally automated solid-phase purification of RNA
The final publication is available at Springer via https://doi.org/10.1007/s10404-014-1477-9.We present a disc-based module for rotationally controlled solid-phase purification of RNA from cell lysate. To this end, multi-stage routing of a sequence of aqueous and organic liquids into designated waste and elution reservoirs is implemented by a network of strategically placed, solvent-selective composite valves. Using a bead-based stationary phase at the entrance of the router, we show that total RNA is purified with high integrity from cultured MCF7 and T47D cell lines, human leucocytes and Haemophilus influenzae cell lysates. Furthermore, we demonstrate the broad applicability of the device through the in vitro amplification of RNA purified on-disc using RT-PCR and NASBA. Our novel router will be at the pivot of a forthcoming, fully integrated and automated sample preparation system for RNA-based analysis.Peer reviewe
Intra‐abdominal hemorrhage following 739 consecutive pancreaticoduodenectomy: Risk factors and treatments
Survival benefit of repeat liver resections for recurrent colorectal metastases: 143 cases. Association Francaise de Chirurgie.
Histological identification of purified and cryopreserved allogeneic hepatocytes following transplantation in a murine model without host immunosuppression
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