Amidoschwarz10B in der forensischen Spurenuntersuchung: Vergleichende Untersuchungen an forensischem Spurenmaterial

Zusammenfassung: Blutspuren gehören zu den aussagekräftigsten Spuren in der forensischen Fallanalyse. Einerseits kann die Beurteilung von Aussehen, Menge, Form und Verteilung an Tatorten Hinweise auf den Tathergang geben. Andererseits lassen sich heute auch aus kleinsten Blutmengen zumeist vollständige DNA-Profile erstellen, die dann ebenfalls eine Rekonstruktion des Tatablaufs und über die molekulargenetische DNA-Analyse Aussagen zur Tatbeteiligung von Personen ermöglichen. In der vorgestellten Studie wurden Vollblutproben auf unterschiedliche Spurenträger aufgebracht und mithilfe des Hexagon OBTI® auf das Vorhandensein von Blut hin untersucht. Nach Inkubation mit 2 verschiedenen Zubereitungen von Amidoschwarz10B konnte gezeigt werden, dass dessen Einsatz zu starken Beeinträchtigungen bzw. falsch-negativen Ergebnissen des OBTI führen kann. Ein negativer Einfluss von Amidoschwarz10B auf das Probenmaterial war hingegen bei den nachfolgend durchgeführten DNA-Analysen nicht festzustelle

Jets in deep-inelastic scattering at HERA

Jet cross sections in deep-inelastic scattering over a wide region of phase space have been measured at HERA. These cross section measurements provide a thorough test of the implementation of Quantum Chromodynamics in next-to-leading order (NLO) calculations. They also provide the opportunity to test the consistency of the gluon distribution in the proton as extracted from (mainly) inclusive DIS measurements. Comparison of the cross sections with NLO enables accurate extractions of the strong coupling constant, $\alpha_s$, to be made, several of which are reported here.Comment: Talk presented at "New Trends in HERA Physics", Ringberg Workshop, June 2001. 13pages, 12figure

On the Perturbative Stability of the QCD Predictions for the Ratio R=FL/FTR=F_L/F_T in Heavy-Quark Leptoproduction

We analyze the perturbative and parametric stability of the QCD predictions for the Callan-Gross ratio $R(x,Q^2)=F_L/F_T$ in heavy-quark leptoproduction. We consider the radiative corrections to the dominant photon-gluon fusion mechanism. In various kinematic regions, the following contributions are investigated: exact NLO results at low and moderate $Q^2\lesssim m^2$, asymptotic NLO predictions at high $Q^2\gg m^2$, and both NLO and NNLO soft-gluon (or threshold) corrections at large Bjorken $x$. Our analysis shows that large radiative corrections to the structure functions $F_T(x,Q^2)$ and $F_L(x,Q^2)$ cancel each other in their ratio $R(x,Q^2)$ with good accuracy. As a result, the NLO contributions to the Callan-Gross ratio are less than 10% in a wide region of the variables $x$ and $Q^2$. We provide compact LO predictions for $R(x,Q^2)$ in the case of low $x\ll 1$. A simple formula connecting the high-energy behavior of the Callan-Gross ratio and low-$x$ asymptotics of the gluon density is derived. It is shown that the obtained hadron-level predictions for $R(x\to 0,Q^2)$ are stable under the DGLAP evolution of the gluon distribution function. Our analytic results simplify the extraction of the structure functions $F_2^c(x,Q^2)$ and $F_2^b(x,Q^2)$ from measurements of the corresponding reduced cross sections, in particular at DESY HERA.Comment: 14 pages, 6 figures, revtex4; minor correction

A global analysis of Y-chromosomal haplotype diversity for 23 STR loci

In a worldwide collaborative effort, 19,630 Y-chromosomes were sampled from 129 different populations in 51 countries. These chromosomes were typed for 23 short-tandem repeat (STR) loci (DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS385ab, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS635, GATAH4, DYS481, DYS533, DYS549, DYS570, DYS576, and DYS643) and using the PowerPlex Y23 System (PPY23, Promega Corporation, Madison, WI). Locus-specific allelic spectra of these markers were determined and a consistently high level of allelic diversity was observed. A considerable number of null, duplicate and off-ladder alleles were revealed. Standard single-locus and haplotype-based parameters were calculated and compared between subsets of Y-STR markers established for forensic casework. The PPY23 marker set provides substantially stronger discriminatory power than other available kits but at the same time reveals the same general patterns of population structure as other marker sets. A strong correlation was observed between the number of Y-STRs included in a marker set and some of the forensic parameters under study. Interestingly a weak but consistent trend toward smaller genetic distances resulting from larger numbers of markers became apparent