LC-MS/MS profile of ACTPG showing the presence of bioactive compounds.

<p>LC-MS/MS profile of ACTPG showing the presence of bioactive compounds.</p

Effects of ACTPG on antiaggregation and disaggregation property of Aβ _25–35.

<p>Analysis of the deposited amyloid aggregates as assessed by Confocal Laser Microscope System (CLSM FV300, Olympus, Tokyo, Japan) in (A) Phase I (20, 48 h) & (B) Phase II (96 h & 9days) and processed by Adobe Photoshop (Adobe Systems, Mountain View, CA, USA). The fluorescence intensity was visualized in each of three random fields of the sample. Confocal microscopic image represents a view of deposited Aβ _25–35 amyloid aggregates, with representative fibrils from Aβ _25–35 samples (control) and Aβ _25–35 samples incubated with the presence and absence of ACTPG and galantamine.</p

Butyrylcholinesterase inhibitory activity of the active compounds (10–50 μg/ml).

<p>The result of BuChE inhibitory activity clearly shows that among the different treatment groups, alpha-bisabolol alone treatment possessed a maximum BuChE inhibitory activity(66%), with the IC50 value <10 μg/ml and showed significant inhibition (*p<0.1) at 50 μg/ml. Further, standard drug donepezil (IC50 value <6 μg/ml) and the compound of our interest, alpha-bisabolol displayed a significant inhibition on BuChE. Values are expressed as Mean ± SD (n = 3).</p

LC-MS/MS spectrum of active fraction (F10) of ACTPG.

<p>LC-MS/MS spectrum of active F10 fraction of ACTPG showed the presence of essential oil alpha-bisabolol.</p

Bioactivity guided fractionation of ACTPG.

<p>Bioactive fractions were eluted with linear gradient of solvent with increasing polarity from n-hexane to water and the 18 fractions (F1-F18) were collected and subjected to cholinesterase inhibition assay.</p

Effects of ACTPG on Aβ _25–35 fibrillogenesis.

<p>Analysis of the deposited amyloid aggregates as assessed by thioflavin T (ThT) assays in Phase I (20, 48 h) & II (96 h & 9days). Quantitative effects of ACTPG on Aβ _25–35 fibrillogenesis by ThT assay showing the bar diagram of thioflavin-T fluorescence of Aβ _25–35 in the presence and absence of ACTPG. The emission spectrum of ACTPG and galantamine alone was subtracted, and emission data of peptide dispersions were normalized. Values are expressed as Mean ± SD (n = 3).</p

AChE inhibitory activity of the column fractions (F1-F18).

<p>The eluted fractions (F1-F18) were subjected to AChE inhibitory assay. Among the fractions, F10 (EA: MET-9:1) showed highest inhibitory activity (93%) against AChE, but similar when compared to positive control donepezil (97%). Values are expressed as Mean ± SD (n = 3).</p