Natural products from filamentous fungi and production by heterologous expression

Filamentous fungi represent an incredibly rich and rather overlooked reservoir of natural products, which often show potent bioactivity and find applications in different fields. Increasing the naturally low yields of bioactive metabolites within their host producers can be problematic, and yield improvement is further hampered by such fungi often being genetic intractable or having demanding culturing conditions. Additionally, total synthesis does not always represent a cost-effective approach for producing bioactive fungal-inspired metabolites, especially when pursuing assembly of compounds with complex chemistry. This review aims at providing insights into heterologous production of secondary metabolites from filamentous fungi, which has been established as a potent system for the biosynthesis of bioactive compounds. Numerous advantages are associated with this technique, such as the availability of tools that allow enhanced production yields and directing biosynthesis towards analogues of the naturally occurring metabolite. Furthermore, a choice of hosts is available for heterologous expression, going from model unicellular organisms to well-characterised filamentous fungi, which has also been shown to allow the study of biosynthesis of complex secondary metabolites. Looking to the future, fungi are likely to continue to play a substantial role as sources of new pharmaceuticals and agrochemicals—either as producers of novel natural products or indeed as platforms to generate new compounds through synthetic biology

Economic Contributions of Colleges and Universities in Maine

This report shows the following: Maine has 38 colleges and universities that educate 72,605 students, employ a combined 14,621 non-student workers, and generate about $2.2 billion in annual revenue. Colleges and universities in Maine generate a total annual economic contribution— including the spending of students and visitors, and multiplier effects—of an estimated$4.5 billion in output, 31,267 full- and part-time jobs (not including student workers), and $1.7 billion in labor income. Maine’s colleges and universities support at least ten jobs in 125 Maine sectors, and there are statewide employment impacts of twenty jobs or more in 108 industrie

Warrant is unique

Abstract Warrant is what fills the gap between mere true belief and knowledge. But a problem arises. Is there just one condition that satisfies this description? Suppose there isn't: can anything interesting be said about warrant after all? Call this the uniqueness problem. In this paper, I solve the problem. I examine one plausible argument that there is no one condition filling the gap between mere true belief and knowledge. I then motivate and formulate revisions of the standard analysis of warrant. Given these revisions, I argue that there is, after all, exactly one warrant condition. Keywords Warrant Á Huemer Á Merricks Á Plantinga Á Knowledge Á Epistemology Background Distinguish two ways of characterizing a condition C. The first (functional analysis) tries to state the role C plays in the relevant (epistemic, moral, etc.) economy. Functional analyses pick out a condition (or conditions) by description; one can imagine a functional analyst saying, 'let C name that condition (or those conditions) that satisfy the following description...'. A second way of characterizing a condition C (substantive analysis) tries to state what C actually is (or at least, when it holds). A substantive analysis is an attempt to formulate the necessary and sufficient conditions of C's being realized. Put differently, whil

Interrogating race: color, racial categories, and class across the Americas

We address long-standing debates on the utility of racial categories and color scales for understanding inequality in the United States and Latin America, using novel data that enable comparisons of these measures across both broad regions. In particular, we attend to the degree to which color and racial category inequality operate independently of parental socioeconomic status. We find a variety of patterns of racial category and color inequality, but that in most countries accounting for maternal education changes our coefficients by 5% or less. Overall, we argue that several posited divergences in ethnoracial stratification processes in the United States, compared with Latin America, might be overstated. We conclude that the comparison of the effects of multiple ethnoracial markers, such as color and racial categories, for the analysis of social stratification holds substantial promise for untangling the complexities of “race” across the Americas

Exploring fungal RiPPs from the perspective of chemical ecology

Since the initial detection, in 2007, of fungal ribosomally synthesised and post-translationally modified peptides (RiPPs), this group of natural products has undergone rapid expansion, with four separate classes now recognised: amatoxins/phallotoxins, borosins, dikaritins, and epichloëcyclins. Largely due to their historically anthropocentric employment in medicine and agriculture, novel fungal proteins and peptides are seldom investigated in relation to the fungus itself. Therefore, although the benefits these compounds confer to humans are often realised, their evolutionary advantage to the fungus, the reason for their continued production, is often obscure or ignored. This review sets out to summarise current knowledge on how these small peptide-derived products influence their producing species and surrounding biotic environment

Why Composition Matters

Many say that ontological disputes are defective because they are unimportant or without substance. In this paper, we defend ontological disputes from the charge, with a special focus on disputes over the existence of composite objects. Disputes over the existence of composite objects, we argue, have a number of substantive implications across a variety of topics in metaphysics, science, philosophical theology, philosophy of mind, and ethics. Since the disputes over the existence of composite objects have these substantive implications, they are themselves substantive