Electrospun Fibers as A Wound Dressing Material using Combination of Cellulose Acetate/Collagen Seeding Stem Cell

Wound healing is a complex tissue regeneration process that the body undergoes as a response to wound openings or missing cellular structures as a result of various types of traumatic injury. Because of the ability of materials to induce a high immune response or limited donor tissues, the skin repair and regeneration methods using allografts and autografts cannot be widely used. Many researches have shifted into tissue engineering approaches using scaffolds. To achieve the goal of tissue reconstruction, scaffolds must meet some specific requirements include biocompatibility, biodegradability, and mechanical properties. Our study aimed to fabricate composite cellulose acetate-collagen (CA/Collagen) scaffolds by electrospinning and determine the appropriate compositions of CA:Collagen for obtaining skin substitutes as wound dressing through investigating the morphological of stem cell seeded on electrospun CA/Collagen membranes. High proliferation of mesenchymal stem cells on electrospun CA/collagen 75:25 (wt.%) confirmed the capability of CA/collagen 75:25 nanofibers as a tissue-engineered scaffold, while the electrospun CA/collagen 75:25 can be a potential low-adherent wound dressing

Descriptive Study About The Difference of miR155-5p and miR125a-3p Expression Value in Breast Cancer Tissues

Background: One of the causative factor in abnormal cell growth in cancer is miRNA abnormality. In our bodies, miRNA act as regulator of our cell. miRNA of cancer in general is divided into 2 types: Oncogenic miRNA, tumor suppressor miRNA. miR-125a is one of the tumor suppressor micro RNA in breast cancer that have role to silence these genes: HuR, HER2, ETS1, Cyclin J, MEGF9. miR-155 is one of the oncogenic micro RNA in breast cancer that have role to silence these genes: SOCS1, TP53INP1, FOXO3, RhoA. Methods: This research uses descriptive method involved 3 samples, 1 of the sample is excluded because it fulfills exclusion factor due to undetermined value on miR155- 5p. This research takes place in tropical disease center during November 2016- Desember 2016. The data collected using breast cancer tissues. Results: The research result based on the research finding shows the differences expression value of miR155-5p and miR125a-3p. Conclusion: Since we do not have the control sample (Healthy tissue) for judging if miR155-5p is higher than normal or miR125a-3p is lower than normal. We can not conclude if the oncogenic micro RNA is greater than tumor suppressor micro RN