Detección del virus papiloma humano (HPV) y citología de Papanicolaou en mujeres de bajos recursos de la ciudad de Posadas, Misiones, Argentina

El objetivo de este estudio fue determinar la prevalencia de la infección por HPV y de lesiones cervicales en mujeres asistidas en un centro de salud situado en un área de bajos recursos de la ciudad de Posadas, Misiones, Argentina. Las muestras (n = 163) fueron examinadas mediante las pruebas de Papanicolaou y de PCR para HPV. Los factores socio-culturales de riesgo fueron identifcados mediante el cálculo de la odds ratio (OR, IC 95 %). Se detectaron lesiones cervicales en el 14,7 % de las mujeres. La prevalencia de infección por HPV fue de 38 %. Los tipos más frecuentes en la población total fueron HPV-16 (9,8 %) y HPV-33 (9,3 %). El HPV-16 se detectó asociado al 29,2 % y al 6,5 % de las mujeres con lesiones del cuello uterino y sin ellas, respectivamente, con un OR de 5,3 (1,8-15,8). Los factores de riesgo para la infección por HPV-16 fueron el hábito de fumar y el antecedente de enfermedades de transmisión sexual. Estos datos son importantes para la ejecución de los programas de prevención, incluyendo una introducción adecuada de la vacunación y la línea de base para la vigilancia virológica en la era de la vacuna.The objective of this study was to determine the prevalence of HPV infection and cervical lesions present in women who attended a health center in a low-resource area of the city of Posadas, Misiones, Argentina. Cervical cell samples (n = 163) were processed for Papanicolaou cytology and HPV-PCR tests. Socio-cultural risk factors were estimated using the odds ratio (OR, CI 95 %). Cervical lesions were detected in 14.7 % of women. The general prevalence of HPV infection was of 38 %. The most common types among the total population were HPV-16 (9.8 %) and HPV-33 (9.3 %). HPV-16 was detected in association with 29.2 % and 6.5 % of women with and without cervical lesions, respectively, the OR being 5.3 (1.8-15.8). Risk factors for HPV-16 infection were a smoking habit and a history of previous sexually-transmitted diseases. These data are important for the implementation of prevention programs, including an appropriate introduction of vaccination and the baseline for virological surveillance in the vaccine era.Fil: Badano, Ines. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; ArgentinaFil: Pedrozo, Rene W.. Provincia de Misiones. Ministerio de Salud de la Provincia de Misiones; ArgentinaFil: Ruiz Diaz, Laura S.. Provincia de Misiones. Ministerio de Salud de la Provincia de Misiones; ArgentinaFil: Galuppo, Juan A.. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; ArgentinaFil: Picconi, María A.. Direccion Nacional de Instituto de Investigacion. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbran". Instituto Nacional de Enfermedades Infecciosas. Departamento de Virologia; ArgentinaFil: Campos, Rodolfo Hector. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Liotta, Domingo Javier. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Laboratorio de Biología Molecular Aplicada; Argentin

X‐ray microtomography and phylogenomics provide insights into the morphology and evolution of an enigmatic mesozoic insect larva

Fossils sometimes show unusual morphological features absent in living organisms, making it difficult to reconstruct both their affinity and their function. We describe here a new lacewing larva, Ankyloleon caudatus gen. et sp.n. (Neuroptera) from the Cretaceous amber of Myanmar, characterized by an abdomen unique among insects, with ‘tail-like’ terminal segments bearing a ventral pair of vesicles. Phase-contrast X-ray microtomography reveals that these structures were dense and equipped with a median duct, suggesting that they were likely pygopods used for locomotion, holding the position through adhesive secretions. Our phylogenetic analyses, combining genomic and morphological data from both living and fossil lacewings, proved critical to placing Ankyloleon gen.n. on the lacewing tree of life as an early representative of the antlion clade, Myrmeleontiformia. These results corroborate the view that derived myrmeleontiform lacewings ‘experimented’ with unusual combinations of features and specializations during their evolutionary history, some of which are now lost

Primosecundarismo sifilítico con chancros extragenitales múltiples en un paciente inmunocompetente

La sífilis es una infección de transmisión sexual causada por la espiroqueta Treponema pallidum subespecie pallidum. Caso clínico: hombre de 28 años sin antecedentes relevantes, que consultó por presentar una placa erosionada infralabial y una lesión tumoral en fosa amigdalina izquierda, asociadas a adenopatía submandibular. Se evidenció un exantema macular en tronco. Con el resultado positivo de la VDRL, se arribó al diagnóstico de primosecundarismo sifilítico con chancros extragenitales múltiples. Tanto los chancros extragenitales como el primosecundarismo sifilítico son poco habituales, siendo más frecuentes en pacientes inmunocomprometidos y con infección por VIH. Aunque las lesiones extragenitales son similares a los chancros genitales, pueden mostrar ciertas características clínicas particulares. Los chancros sifilíticos localizados en piel son extremadamente raros, se han reportado pocos casos en la literatura. El diagnóstico se realiza en base a la sospecha clínica junto con las pruebas de serología positivas. El tratamiento de elección es el uso de penicilina G benzatínica intramuscular.Syphilis is a sexually transmitted infection caused by Treponema pallidum subspecies pallidum. Clinical case: An otherwise healthy 28-year-old male with an ulcerative plaque on his lower lip, a tumoral lesion in the left tonsillar fossa and a left submandibular adenopathy. Physical examination revealed a macular rash on the trunk. VDRL was positive, thus confirming the diagnosis of coexistence of primary and secondary syphilis with multiple extragenital chancre. Extragenital chancres and the overlap between the primary and secondary stage of syphilis is uncommon, being more frequent in immunocompromised and HIV positive patients. Although extragenital lesions are similar to genital chancres, they can have some particular clinical characteristics. The skin syphilitic chancres are extremely rare, and few cases have been described in the literature. Diagnosis depends on clinical suspicion combined with positive serology testing. Benzathine penicillin G is the recommended treatment.Fil: Sánchez Martini, Pía Florencia. Hospital Luis Lagomaggiore (Mendoza, Argentina). Servicio de DermatologíaFil: Cantú Parra, Laura. Hospital Luis Lagomaggiore (Mendoza, Argentina). Servicio de DermatologíaFil: García Llaver, Valeria. Hospital Alfredo Ítalo Perrupato (Mendoza, Argentina)Fil: Innocenti Badano, Alicia Carolina. Hospital Luis Lagomaggiore (Mendoza, Argentina). Servicio de DermatologíaFil: Tennerini, María Leticia. Hospital Luis Lagomaggiore (Mendoza, Argentina). Servicio de Dermatologí

Controlled HIV-HCV Viremia and Immune-Reconstitution are Associated with Slow Progression of Liver Disease in Co-infected Hemophilic Patients After 30 Years of Follow-Up

Introduction and aim: Controversial results have been reported about the progressionof liver disease in HIV-HCV coinfected populations. The purpose of this study is to assesslong-term liver disease progression in a group of coinfected patients with hemophilia.1.2. Materials and Methods: From 1995 to 2015, liver disease was assessed through enzymelevels, platelet counts, Hepatitis C and HIV viral loads (VL), and CD4+T cell counts. Evolution of the APRI liver index was used to estimate hepatic disease (APRI > 1.0 indicatingsevere fibrosis).1.3. Results: 2005-2015 proportional liver-related mortality was below 17% while AIDSand other causes including hemorrhagic events reached 42% each. APRI index >1.0 wasfound in 3 of 32 (9%) patients alive, showing significant liver disease after more than 30years of infection. Analyzing the evolution of liver disease markers, liver enzymes increasedsignificantly only in those patients with detectable HIV and /or HCV VL (for AST and ALT,p<0.0001; for GGT, p=0.001). HIV suppression and reconstitution of CD4+T cell countswere required to achieve HCV eradication. Through multivariate logistic regression, pre ART(pre-antiretroviral therapy) HIV VL was associated with the development of liver fibrosis(OR=4.755; IC95: 1.057 21.387) and with altered liver enzyme values (OR=4.091; IC95:1.293 ? 12.947). No persistent increase in enzyme levels or APRI index was observed in thegroup controlling HIV and HCV replication and adequate immune recovery.1.4. Conclusions: The suppression of both viruses, HIV and HCV, together with adequateimmune recovery is associated with minimal or slow progression of liver disease.Fil: Badano, Maria Noel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Monzani, María Cecili. Academia Nacional de Medicina de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Aloisi Zavala, Natalia Andrea. Academia Nacional de Medicina de Buenos Aires; ArgentinaFil: Gualtieri, Ariel Félix. Academia Nacional de Medicina de Buenos Aires; ArgentinaFil: Corti, Marcelo. Fundacion de la Hemofilia; ArgentinaFil: Parodi, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Pinto, Tezanos. Academia Nacional de Medicina de Buenos Aires; ArgentinaFil: Primiani, Laura. Fundacion de la Hemofilia; ArgentinaFil: Bracco, M. M. E. D.. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Chuit, Roberto. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Epidemiológicas; ArgentinaFil: Baré, Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentin

Tungiasis : enfermedad tropical con manifestación cutánea

La tungiasis es una ectoparasitosis prevalente en Sudamérica, el Caribe y algunas regiones de África. Clínicamente se manifiesta como pápulas o nódulos, únicos o múltiples, blanquecinos o grisáceos, que reflejan el desarrollo de la pulga. El diagnóstico se basa fundamentalmente en el antecedente epidemiológico, la clínica y la dermatoscopia. El tratamiento de elección es la extracción quirúrgica de la pulga, al que se le suma la profilaxis antitetánica.Tungiasis is an ectoparasitosis, prevalent in South America, the Caribbean and some regions of Africa. Clinical findings are papules or nodules, single or multiple, whitish or grayish, which reflect the development of the flea. The diagnosis is fundamentally based on the epidemiological history, the skin lesions and the dermoscopy. Standard treatment consists of surgical extraction of the flea followed by tetanus prophylaxis.Fil: Camardella, Ileana. Hospital Luis Lagomaggiore (Mendoza, Argentina). Servicio de DermatologíaFil: Boulet, María Julia. Hospital Luis Lagomaggiore (Mendoza, Argentina). Servicio de DermatologíaFil: Cantú Parra, Laura. Hospital Luis Lagomaggiore (Mendoza, Argentina). Servicio de DermatologíaFil: Cheseé, Carla. Hospital Luis Lagomaggiore (Mendoza, Argentina). Servicio de DermatologíaFil: Rivero, Lorena. Hospital Luis Lagomaggiore (Mendoza, Argentina). Servicio de MicrobiologíaFil: Borzotta, Florencia. Hospital Luis Lagomaggiore (Mendoza, Argentina). Servicio de DermatologíaFil: Innocenti Badano, Alicia Carolina. Hospital Luis Lagomaggiore (Mendoza, Argentina). Servicio de Dermatologí

Miasis cutánea foruncular : presentación de un caso autóctono en Mendoza

La miasis foruncular es una infestación cutánea infrecuente en la provincia de Mendoza. Es causada por larvas de moscas de la especie Dermatobia hominis. Clínicamente, se manifiesta como pápulas o nódulos eritematosos con orificio central, correspondiente al poro respiratorio de la larva. Presentamos el caso de un hombre de 49 años que consultó por nódulos eritematosos con sensación de movimiento en su interior, localizados en axila derecha. Es importante sospechar este diagnóstico en pacientes con lesiones símil forúnculos, para indicar un tratamiento precoz y evitar complicaciones.Furuncular myiasis is a skin infestation rarely observed in Mendoza. It is caused by the larvae of Dermatobia hominis. Clinically, it manifests as erythematous papules or nodules with a central orifice, corresponding to the respiratory pore of the larva. An otherwise healthy 49-year-old man who presented with erythematous nodules with a sensation of movement inside, located on the right axilla. It is important to suspect this diagnosis in patients with furuncular-type lesions, to indicate an accurate treatment and avoid complications.Fil: Camardella, Ileana. Hospital Luis Lagomaggiore (Mendoza, Argentina). Servicio de DermatologíaFil: Sánchez Martini, Pía Florencia. Hospital Luis Lagomaggiore (Mendoza, Argentina). Servicio de DermatologíaFil: Morea, Gastón. Hospital Luis Lagomaggiore (Mendoza, Argentina). Sección de InfectologíaFil: Cantú Parra, Laura. Hospital Luis Lagomaggiore (Mendoza, Argentina). Servicio de DermatologíaFil: Innocenti Badano, Alicia Carolina. Hospital Luis Lagomaggiore (Mendoza, Argentina). Servicio de DermatologíaFil: Tennerini, María Leticia. Hospital Luis Lagomaggiore (Mendoza, Argentina). Servicio de Dermatologí

Síndrome de Wells : reporte de un caso

El Síndrome de Wells o celulitis eosinofílica, es una dermatosis infrecuente de etiología desconocida. Se lo clasifica dentro de las enfermedades eosinofílicas, ya que presentan en la histopatología infiltración por dichas células. Se han reportado casos asociados a múltiples entidades, como infecciones, neoplasias y fármacos. Clínicamente se manifiesta con pápulas y placas eritematosas, de bordes definidos. La mayoría evidencia una buena respuesta al tratamiento con corticoides sistémicos y puede evolucionar con períodos de brotes y remisiones.Wells syndrome, also known as eosinophilic cellulitis, is a rare dermatosis of unknown etiology. It belongs to the eosinophilic skin diseases, characterized by an eosinophil-rich infiltrate in histopathology. Most cases have been reported associated with multiple entities, such as infections, neoplasms and drugs. Typical findings include infiltrated erythematous papules and plaques. Most patients benefit from systemic corticosteroid therapy and can evolve with alternating periods of flares and remissions.Fil: Arzalluz, Gabriela. Hospital Luis Lagomaggiore (Mendoza, Argentina). Servicio de DermatologíaFil: Camardella, Ileana Rosalía. Hospital Luis Lagomaggiore (Mendoza, Argentina). Servicio de DermatologíaFil: Boulet, María Julia. Hospital Luis Lagomaggiore (Mendoza, Argentina). Servicio de DermatologíaFil: Cantú Parra, Laura. Hospital Luis Lagomaggiore (Mendoza, Argentina). Servicio de DermatologíaFil: Cippitelli, María José. Hospital Luis Lagomaggiore (Mendoza, Argentina). Servicio de DermatologíaFil: Innocenti Badano, Alicia Carolina. Hospital Luis Lagomaggiore (Mendoza, Argentina). Servicio de Dermatologí

A novel form of deleted in breast cancer 1 (DBC1) lacking the N-terminal domain does not bind SIRT1 and is dynamically regulated in vivo

The protein Deleted in Breast Cancer-1 is a regulator of several transcription factors and epigenetic regulators, including HDAC3, Rev-erb-alpha, PARP1 and SIRT1. It is well known that DBC1 regulates its targets, including SIRT1, by protein-protein interaction. However, little is known about how DBC1 biological activity is regulated. In this work, we show that in quiescent cells DBC1 is proteolytically cleaved, producing a protein (DN-DBC1) that misses the S1-like domain and no longer binds to SIRT1. DN-DBC1 is also found in vivo in mouse and human tissues. Interestingly, DN-DBC1 is cleared once quiescent cells re-enter to the cell cycle. Using a model of liver regeneration after partial hepatectomy, we found that DN-DBC1 is down-regulated in vivo during regeneration. In fact, WT mice show a decrease in SIRT1 activity during liver regeneration, coincidentally with DN-DBC1 downregulation and the appearance of full length DBC1. This effect on SIRT1 activity was not observed in DBC1 KO mice. Finally, we found that DBC1 KO mice have altered cell cycle progression and liver regeneration after partial hepatectomy, suggesting that DBC1/DN-DBC1 transitions play a role in normal cell cycle progression in vivo after cells leave quiescence. We propose that quiescent cells express DN-DBC1, which either replaces or coexist with the full-length protein, and that restoring of DBC1 is required for normal cell cycle progression in vitro and in vivo. Our results describe for the first time in vivo a naturally occurring form of DBC1, which does not bind SIRT1 and is dynamically regulated, thus contributing to redefine the knowledge about its function

Predicting Quantitative Genetic Interactions by Means of Sequential Matrix Approximation

Despite the emerging experimental techniques for perturbing multiple genes and measuring their quantitative phenotypic effects, genetic interactions have remained extremely difficult to predict on a large scale. Using a recent high-resolution screen of genetic interactions in yeast as a case study, we investigated whether the extraction of pertinent information encoded in the quantitative phenotypic measurements could be improved by computational means. By taking advantage of the observation that most gene pairs in the genetic interaction screens have no significant interactions with each other, we developed a sequential approximation procedure which ranks the mutation pairs in order of evidence for a genetic interaction. The sequential approximations can efficiently remove background variation in the double-mutation screens and give increasingly accurate estimates of the single-mutant fitness measurements. Interestingly, these estimates not only provide predictions for genetic interactions which are consistent with those obtained using the measured fitness, but they can even significantly improve the accuracy with which one can distinguish functionally-related gene pairs from the non-interacting pairs. The computational approach, in general, enables an efficient exploration and classification of genetic interactions in other studies and systems as well