9 research outputs found
Estimation of the genetic contribution of presenilin-1 and -2 mutations in a population-based study of presenile Alzheimer disease
Two closely related genes, the presenilins ( PS ), located at chromosomes
14q24.3 and 1q42.1, have been identified for autosomal dominant Alzheimer
disease (AD) with onset age below 65 years (presenile AD). We performed a
systematic mutation analysis of all coding and 5'-non-coding exons of PS
-1 and PS -2 in a population-based epidemiological series of 101 unrelated
familial and sporadic presenile AD cases. The familial cases included 10
patients of autosomal dominant AD families sampled for linkage analysis
studies. In all pat
Study of the origin of nondisjunction in a family with 2 cases of down-syndrome using cytogenetic and molecular polymorphisms
A population-based study of familial Alzheimer's disease: linkage to chromosome 14, 19 and 21
Linkage of Alzheimer disease (AD) to DNA markers on chromosomes 14, 19, and 21 was studied in 10 families in which the disease was apparently inherited as an autosomal dominant trait. Families were derived from a Dutch population-based epidemiologic study of early-onset AD. Although in all probands the onset of AD was at or before age 65 years, the mean age at onset was after age 65 years in four families (referred to as "LOAD"). Among the six families with early-onset AD (referred to as "EOAD," i.e., mean age of onset of AD of relatives was at or before age 65 years), conclusive linkage to 14q24.3 was found in one family with a very early onset (around 47 years), while linkage to the same region was excluded in two other families. For the LOAD families, predominantly negative lod scores were obtained, and the overall lod score excluded linkage to chromosome 14. The results with markers on chromosome 19 and chromosome 21 were not conclusive for EOAD and LOAD. The findings of our study confirm genetic heterogeneity within familial EOAD
Genetic variability in the regulatory region of presenilin 1 associated with risk for Alzheimer's disease and variable
Mutations in the presenilin 1 ( PSEN1 ) gene have been implicated in
18-50% of autosomal dominant cases with early-onset Alzheimer's disease
(EOAD). Also, PSEN1 has been suggested as a potential risk gene in
late-onset AD cases. We recently showed genetic association in a
population-based study of EOAD, pointing to the 5' regulatory region of
PSEN1. In this study we systematically screened 3.5 kb of the PSEN1
upstream region and found four novel polymorphisms. Genetic analysis
confirmed association of two polymorphisms with increased risk for EOAD.
In addition, we detected two different mutations in EOAD cases at-280
and-2818 relative to the transcription initiation site in exon 1A of
PSEN1. Analysis of the mutant and wild-type-280 variants using luciferase
reporter gene expression in transiently transfected neuroblastoma cells
showed a 30% decrease in transcriptional activity for the mutant-280G
PSEN1 promoter variant compared with the wild-type variant-280C. Our data
suggest that the increased risk for EOAD associated with PSEN1 may result
from genetic variations in the regulatory region leading to altered
expression levels of the PSEN1 protein
Aberrant splicing in the presenilin-1 intron 4 mutation causes presenile Alzheimer's disease by increased Aβ42 secretion
We previously described a splice donor site mutation in intron 4 of presenilin-1 (PSEN1) in two patients with autopsy-confirmed early-onset Alzheimer's disease (AD), Here we provide evidence that the intron 4 mutation is present in four additional unrelated early-onset AD cases, that the mutation segregates in an autosomal dominant manner and that all cases have one common ancestor. We demonstrate that the intron 4 mutation produces three different transcripts, two deletion transcripts (Delta 4 and Delta 4(cryptic)) and one insertion transcript (ins(TAC)), by aberrant splicing, The deletion transcripts result in the formation of C-truncated (similar to 7 kDa) PSEN1 proteins while the insertion transcript produces a full-length PSEN1 with one extra amino acid (Thr) inserted between codons 113 and 114 (PSEN1 T113-114ins). The truncated proteins were not detectable in vivo in brain homogenates or lymphoblast lysates of mutation carriers. In vitro HEK-293 cells overexpressing Delta 4, Delta 4(cryptic) or ins(TAC) PSEN1 cDNAs showed increased A beta 42 secretion (similar to 3.4 times) only for the insertion cDNA construct. Increased A beta 42 production was also observed in brain homogenates, Our data indicate that in the case of intron 4 mutation, the AD pathophysiology results from the presence of the PSEN1 T113-114ins protein comparable with cases carrying dominant PSEN1 missense mutations
Mapping and sequencing the chromosome 14q24.3 dihydrolipoyl succinyltransferase (DLST) gene in patients with early-onset Alzheimer's disease
Linkage analysis studies have indicated that the chromosome band 14q24.3 harbours a major gene for familial early-onset Alzheimer's disease (AD). Recently we localized the chromosome 14 AD gene (AD3) in the 6.4 cM interval between the markers D14S289 and D14S61. We mapped the gene encoding dihydrolipoyl succinyltransferase (DLST), the E2k component of human alpha-ketoglutarate dehydrogenase complex (KGDHC), in the AD3 candidate region using yeast artificial chromosomes (YACs). The DLST gene is a candidate for the AD3 gene since deficiencies in KGDHC activity have been observed in brain tissue and fibroblasts of AD patients. The 15 exons and the promoter region of the DLST gene were analysed for mutations in chromosome 14 linked AD cases and in two series of unrelated early-onset AD cases (onset age < 55 years). Sequence variations in intronic sequences (introns 3, 5 and 10) or silent mutations in exonic sequences (exons 8 and 14) were identified. However, no AD related mutations were observed, suggesting that the DLST gene is not the chromosome 14 AD3 gene
Subregional localization of the chromosome 21 loci D21S24 and D21S26 using physical mapping techniques
Genetic-linkage studies suggest that alzheimers-disease is not a single homogeneous disorder
Genetic-linkage studies suggest that alzheimers-disease is not a single homogeneous disorder
Alzheimer's disease, a fatal neurodegenerative disorder of unknown aetiology, is usually considered to be a single disorder because of the general uniformity of the disease phenotype. Two recent genetic linkage studies revealed co-segregation of familial Alzheimer disease with the D21S1/S11 and D21S16 loci on chromosome 21. But two other studies, one of predominantly multiplex kindreds with a late age-of-onset, the other of a cadre of kindreds with a unique Volga German ethnic origin, found absence of linkage at least to D21S1/S11. So far it has not been possible to discern whether these conflicting reports reflect aetiological heterogeneity, differences in methods of pedigree selection, effects of confounding variables in the analysis (for example, diagnostic errors, assortative matings), or true non-replication. To resolve this issue, we have now examined the inheritance of five polymorphic DNA markers from the proximal long arm of chromosome 21 in a large unselected series of pedigrees with familial Alzheimer's disease. Our data suggest that Alzheimer's disease is not a single entity, but rather results from genetic defects on chromosome 21 and from other genetic or nongenetic factors