A large synoptic dataset of coastal in situ observations

Since the beginning of 2004 MUMM has performed a series of moorings in the Belgian Coastal Zone with a tripod or benthic lander, equipped with a series of oceanographic sensors. Current meters such as an Acous1c Doppler Profiler (ADP) and an Acoustic Doppler Velocimeter (ADV), a CT-­sensor capable of measuring temperature and salinity, op1cal (OBS) and acous1c backsca\er sensors (ADP and ADV) to measure suspended sediment concentra1on, a LISST 100 suspended par1cle sizer, a passive Cetacean monitoring device (C-­PoD) and a passive sampling device for chemical monitoring have ever since been moored for more than 1750 days. From autumn 2009 a permanent coastal observatory has been installed at MOW1, located to the west of the entrance to the Zeebrugge harbor. Other mooring sites include more offshore loca1ons, such as the Kwintebank, Gootebank, MOW0 and the offshore windmill farms and a very nearshore loca1on (Blankenberge)

Stability of far field R wave signals in different conditions

Aims The presence of far field R wave sensing (FFRS) is usually evaluated in patients with dual chamber pacemakers in supine position. To check if this approach is valid, we tested whether FFRS is consistent both in terms of amplitude threshold and timing characteristics in different daily life conditions. Methods and Results In 42 patients with a DDD pacemaker. the presence. amplitude threshold and timing parameters of FFRS were therefore determined. with patients supine. standing and at peak exercise. Measurements were made of paced and sensed R waves in unipolar and bipolar sensing configurations (at peak exercise only paced R waves and bipolar sensing). After paced R waves (bipolar sensing) amplitude thresholds/time of FFRS after Vpace were 0.32 +/- 0.18 mV/119-139 ms (supine). 0.32 +/- 0.16 mV/114-130 ms (upright) and 0.27 +/- 0.13 mV/121-136 ms (exercise) - with unipolar sensing. this was 0.49 +/- 0.27mV/101-150 ms (Supine). 0.51 +/- 0.29 mV/100-144 ms (upright). After sensed R waves (bipolar sensing) amplitude thresholds/time of FFRS after Vsense were 0.27 +/- 0.18 mV/ 24-42 ms (Supine). 0.29 +/- 0.16 mV/18 to 41 ms (upright) - with unipolar sensing. thresholds were 0.59 +/- 0.32 mV/3-50 ins (supine). 0.59 +/- 0.36 mV/2-58 ms (upright). Conclusion given the lower FFRS thresholds with bipolar sensing. bipolar sensing is superior in avoiding FFRS compared with Unipolar sensing. No differences were found in terms of amplitude thresholds and timing characteristics with patients supine. standing and at peak exercise. Thus. measurements made in the supine position Lire basically sufficient to predict the presence/absence of FFRS under different conditions

Synthesis of D- and L-myo-inositol 1,2,4,6-tetrakisphosphate, regioisomers of myo-inositol 1,3,4,5 tetrakisphosphate: activity against Ins(1,4,5)P3 binding proteins.

We report here the synthesis of D- and L-myo-inositol 1,2,4,6-tetrakisphosphate 3a and 3b and the racemic modification 3ab. Racemic myo-inositol 1,2,4,6-tetrakisphosphate 3ab was synthesised from DL-1,2,4,6-tetra-O-allyl-myo-inositol 9ab. Benzylation and de-allylation provided the tetraol 11ab, which was phosphitylated in the presence of bis(benzyloxy)diisopropylaminophosphine and 1H-tetrazole, then oxidised to give the fully protected 1,2,4,6-tetrakisphosphate 13ab. Hydrogenolysis of 13ab and purification of product by ion exchange chromatography gave racemic myo-inositol 1,2,4,6-tetrakisphosphate 3ab, which showed no demonstrable agonism or antagonism for Ca2+ release at 200 microM in permeabilised hepatocytes. The chiral derivatives, D-3a and L-myo-inositol 1,2,4,6-tetrakisphosphate 3b were synthesised from 5-O-benzyl-1,4,6-tri-O-p-methoxybenzyl-myo-inositol 19ab, which was resolved using R-(-)-O-acetylmandelic acid providing two diastereoisomers 21 and 22 which were separated and deacylated to give the corresponding enantiomers. Further transformations gave the corresponding chiral 1,2,4,6-tetraols which were phosphitylated, oxidised, deprotected and purified as for the racemic mixture. The enantiomeric tetrakisphosphates 3a and 3b were evaluated for inhibition of the metabolic enzymes inositol 1,4,5-trisphosphate 5-phosphatase and 3-kinase in comparison with the enantiomers of another synthetic regioisomer D- and L-myo-inositol 1,2,4,5-tetrakisphosphate. Both D- and L-myo-inositol 1,2,4,6-tetrakisphosphate inhibit 5-phosphatase with an IC50 value of 3.8 microM and 14 microM, repectively. However, both enantiomers were poorly recognised by the 3-kinase enzyme, with IC50 values greater than 100 microM. The enantiomers of the 1,2,4,5-tetrakisphosphate showed the same relative pattern of activity towards the two enzymes but were more potent against 5-phosphatase (0.47 microM and 3 microM respectively).Journal ArticleResearch Support, Non-U.S. Gov'tinfo:eu-repo/semantics/publishe