Selective laser photolysis of organic molecules in complex matrices

No abstract availabl

Study of the phenotypic and genotypic biodiversity of potentially ochratoxigenic black aspergilli isolated from grapes

Int. J. Food Microbiol. ISI Document Delivery No.: 454WC Times Cited: 7 Cited Reference Count: 45 Dachoupakan, C. Ratomahenina, R. Martinez, V. Guiraud, J. P. Baccou, J. C. Schorr-Galindo, S. Elsevier science bv AmsterdamInternational audienceOchratoxin A (OTA) is a mycotoxin with nephrotoxic, carcinogenic, teratogenic and immunotoxic effects, naturally found in agricultural products including grapes and wine. Black Aspergillus species (Section Nigri) are mainly responsible for OTA accumulation in wine grapes and in particular Aspergillus carbonarius and Aspergillus niger aggregate. The biodiversity of potentially ochratoxigenic strains of black aspergilli from different French vineyards in the southern Mediterranean region of Languedoc-Roussillon was studied. One hundred and eighty nine black strains were isolated from grapes and studied according to harvest year, production zone. grape variety and pre-harvest treatment of grapevines. The strains were identified and classified in two groups according to macroscopic and microscopic characters; these were called the A. carbonarius representative group and the A. niger aggregate representative group. Members of each group were classified in subgroups based on macroscopic morphological colony characters. Strain biodiversity was studied according to phenotypic and genotypic characterization and to the OTA production of selected strains on PDA medium. After identification was confirmed by specific PCR using primer pair ITS1/CAR and ITS1/NIG, 24 potential ochratoxigenic strains belonging to A. carbonarius and A. niger aggregate were discriminated by RAPD-PCR using 8 different OPC primers. The use of specific primers supported the identification based on phenotypic and morphological characters. RAPD-PCR patterns demonstrated a considerable diversity among the strains. Clustering among A. niger aggregate strains was associated with production zone and harvest year, but not grape variety or pre-harvest treatment. Clustering among A. carbonanius strains was not associated with any of the above parameters. OTA production of strains on culture medium seemed to correlate better with morphological characters than with genotypic profiles. No clear relation could be established between phenotypic and genotypic characters of the studied black aspergilli. (C) 2009 Elsevier B.V. All rights reserved

Pourridie et deperissement du jasmin. Approche par les techniques de culture in vitro et de culture hors sol et approche diagnostic. Rapport final 1990

* INRA, Unite Regionale de Documentation, Montfavet (FRA) Diffusion du document : INRA, Unite Regionale de Documentation, Montfavet (FRA

Efficacité de la réduction de la masse des nutriments dans la prévention des malaïgues, application aux étangs palavasiens

[Departement_IRSTEA]GMA [TR1_IRSTEA]GMA4-Ressources aquatiques vivantes exploitéesCe document de synthèse était originellement accompagné des annexes suivantes : I-A. Évaluation des apports en azote et en phosphore des bassins versants (données statistiques et bibliographiques). Application aux etangs du departement de l'Hérault. I-B. Évaluation des apports en azote et en phosphore des bassins versants (approche préliminaire des mesures de flux). Application au bassin versant du Salaison. II. Cartographie et estimation des stocks de nutriments dans les sediments. Application aux étangs du complexe palavasien. III-A. Le fractionnement du phosphore en milieu saumâtre (etude bibliographique et comparaison de methodes). Application aux sediments de l'etang de l'Or. III-B. Échanges de phosphore eau-sediment en milieu saumatre (mesures du flux diffusif par peepers). Application aux etangs de l'Or et du Prevost. IV. Recherche de la limitation de Ia croissance des ulves par les nitrates et les phosphates (étude de terrain et de laboratoire). Application a l'etang du Prevost. V. Interventions pour reduire Ia masse de nutriments. Actions sur les bassins versants, les sédiments et les algues (analyse bibliographique et enquêtes). Application technique et économique aux lagunes palavasiennes. Le document numérisé joint ne comprend que les annexes, I-A, II, III-A, III-