Parallel analysis of tri-molecular biosynthesis with cell identity and function in single cells.

Cellular products derived from the activity of DNA, RNA, and protein synthesis collectively control cell identity and function. Yet there is little information on how these three biosynthesis activities are coordinated during transient and sparse cellular processes, such as activation and differentiation. Here, we describe Simultaneous Overview of tri-Molecule Biosynthesis (SOM3B), a molecular labeling and simultaneous detection strategy to quantify DNA, RNA, and protein synthesis in individual cells. Comprehensive interrogation of biosynthesis activities during transient cell states, such as progression through cell cycle or cellular differentiation, is achieved by partnering SOM3B with parallel quantification of select biomolecules with conjugated antibody reagents. Here, we investigate differential de novo DNA, RNA, and protein synthesis dynamics in transformed human cell lines, primary activated human immune cells, and across the healthy human hematopoietic continuum, all at a single-cell resolution

Focal Mechanism and Rupture Process of 2004 Alhoceima (Morocco, Mw=6.2) Earthquake from Teleseismic and Regional Broad-Band Data.

We have study the focal mechanism of the 2004 Alhoceima (Morocco, Mw=6.2) earthquake using teleseismic and regional broad-band data. The solution obtained shows strike slip motion with planes striking respectively on NNE-SSW and WNW-ESE direction and horizontal pressure axes in NNW-SSE direction. We inverted body waves at teleseismic distances using as initial orientation the solution obtained from 126 P polarities. A model of extended source with rupture velocity between 2.5-3.0 km was used for the inversion. We find a complex rupture with four events at shallow depth (2-8 km). The rupture started at 6 km depth and propagated toward the south with maximum seismic moment releases at the first step (80% over a total of 1.8 x10e18 Nm). Similar result was obtained from slip inversion. An aftershock occurred on 12/03/04 (Mw=4.8) was used as empirical green function using broad-band data at re gional distances (40 to 300 km) to estimate the source time function. Comparison of these results with those obtained for the 1994 earthquake show similar behaviour, namely, a complex rupture process and apparently no relation of the 1994 and 2004 shocks with the Nekor fault, the most important geological feature in the studied area. The stress pattern derived from the 1994 and 2004 focal mechanisms are in agreement with the regional stress pattern in the Alboran Sea: horizontal compres- sion in NNW-SSE and horizontal extension in E-W direction

Generalising the logistic map through the qq-product

We investigate a generalisation of the logistic map as $x_{n+1}=1-ax_{n}\otimes_{q_{map}} x_{n}$ ($-1 \le x_{n} \le 1$, $0<a\le2$) where $\otimes_q$ stands for a generalisation of the ordinary product, known as $q$-product [Borges, E.P. Physica A {\bf 340}, 95 (2004)]. The usual product, and consequently the usual logistic map, is recovered in the limit $q\to 1$, The tent map is also a particular case for $q_{map}\to\infty$. The generalisation of this (and others) algebraic operator has been widely used within nonextensive statistical mechanics context (see C. Tsallis, {\em Introduction to Nonextensive Statistical Mechanics}, Springer, NY, 2009). We focus the analysis for $q_{map}>1$ at the edge of chaos, particularly at the first critical point $a_c$, that depends on the value of $q_{map}$. Bifurcation diagrams, sensitivity to initial conditions, fractal dimension and rate of entropy growth are evaluated at $a_c(q_{map})$, and connections with nonextensive statistical mechanics are explored.Comment: 12 pages, 23 figures, Dynamics Days South America. To be published in Journal of Physics: Conference Series (JPCS - IOP