Utilization of winery wastes for Trichoderma viride biocontrol agent production by solid state fermentation

Copyright © 2008 The Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences Published by Elsevier B.V.Biocontrol agents are safe and environmental friendly alternatives for pesticides in agriculture application. Trichoderma viride WEBL0703 performed a high level of antagonistic activity toward a broad spectrum of phytopathogens and was determined as a biocontrol agent, which was produced by solid state fermentation using grape marc and wine lees. The maximum yield of T. viride conidia was up to 6.65 x 10(9) CFU/g initial dry substrate (IDS) after 10 d fermentation. As important enzymes for protecting plants from disease, chitinase, beta-glucanase, and pectinase yields were 47.8 U/g IDS, 8.32 U/g IDS and 9.83 U/g IDS, respectively. These results show that it is feasible to convert winery wastes to a value-added and environmental friendly biocontrol agent.Zhihui Bai, Bo Jin, Yuejie Li, Jian Chen and Zuming Lihttp://www.elsevier.com/wps/find/journaldescription.cws_home/709941/description#descriptio