19 research outputs found
Norbornene-chitosan spray-dried microspheres for peptide conjugation using thiol-ene âphotoclickâ chemistry
This work was financed by Portuguese funds through FCT/MCTES (Fundação para a CiĂȘncia e a Tecnologia/MinistĂ©rio da CiĂȘncia, Tecnologia e Inovação) in the framework of the projects 2022.06048.PTDC (i3S), UIDB/50006/2020 (LAQV-REQUIMTE), LA/P/0045/2020 (ALiCE) and UIDB/00511/2020 (LEPABE). P.A. (SFRH/BD/145471/2019) and D.F. (SFRH/ BD/146890/2019) doctoral grants, were financially supported by national (FCT/Norte 2020 Framework) and European Union (ESF â European Social Fund) funds. B.E. acknowledges FCT for the contract based on the âLei do Emprego CientĂficoâ (DL 57/2016). Maria Cristina L. Martins also acknowledges FCT (LA/P/0070/2020), project Bio2Skin Advanced (2021-24):NORTE-01-0247-FEDER-047225; and MOBILIsE Project, which has received funding from the European Unionâs Horizon 2020 research and innovation program under grant agreement no. 951723.The action of bioactive peptides, such as antimicrobial peptides (AMP), in the human body
is often compromised by limited residence time and stability in the target site. Bioconjugation of
peptides to biomaterial surfaces is one of the strategies that may overcome these limitations. Herein,
norbornene-chitosan (NorChit) microspheres were engineered to react with thiolated peptides by thiolene âphotoclickâ chemistry. NorChit microspheres were produced by spray drying and crosslinked
with dithiothreitol (DTT) to prevent their solubilization. Microspheres with a diameter of 5 ± 2 ”m
showed round and smooth morphology with pockets over the surface that could be related with
hydrophobic interactions between internal norbornene groups. Thiol-ene bioconjugation carried
out using a fluorescent model peptide, showed a yield of 45%, whereas using the peptide but without
UV exposure indicated a maximum of peptide adsorption of 30%. Altogether, NorChit microspheres
show the potential for carrying bioactive peptides, which may open avenues for AMP activity onto
harsh environments in the bod
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Preparation of chitosan nanoparticles by spray drying and their antibacterial activity
[[abstract]]Chitosan nanoparticles were prepared from chitosan with different molecular weight by spray drying method. The morphology of chitosan nanoparticles were characterized by SEM and size distribution and zeta potential values were determined. Effect of chitosan solution concentrations, molecular weight of chitosan (MMW, HMW and VHMW) and size of spray dryer nozzles on average size, size distribution and zeta potential values of chitosan nanoparticles were investigated. Moreover, the effect chitosan nanoparticles and chitosan nanoparticles/amoxicillin complex on Staphylococcus aureus was also tested. The results showed that the average size of chitosan nanoparticles were in the range of 95.5 to 395 nm and zeta potential values of 39.3 to 45.7 mV depended on concentration and molecular weight of chitosan. The lower concentration and molecular weight of chitosan were used, the smaller size of chitosan nanoparticles and the higher zeta potential values were obtained. The testing for antibacterial activity against S. aureus indicated that chitosan nanoparticles strongly inhibited the growth of bacteria with the minimum inhibitory concentration (MIC) of 20”g/mL, which were lower than that of chitosan solution and amoxicillin. The antibacterial capacity of chitosan nanoparticles also depended on size, zeta potential values and molecular weight of chitosan. Complex of chitosan nanoparticles/amoxicillin could improve antibacterial activity of amoxicillin.[[notice]]èŁæŁćźçą[[incitationindex]]SCI[[booktype]]çŽæŹ[[booktype]]é»ć