14 research outputs found
Social preferences and network structure in a population of reef manta rays
Understanding how individual behavior shapes the structure and ecology ofpopulations is key to species conservation and management. Like manyelasmobranchs, manta rays are highly mobile and wide ranging species threatened byanthropogenic impacts. In shallow-water environments these pelagic rays often formgroups, and perform several apparently socially-mediated behaviors. Group structuresmay result from active choices of individual rays to interact, or passive processes.Social behavior is known to affect spatial ecology in other elasmobranchs, but this isthe first study providing quantitative evidence for structured social relationships inmanta rays. To construct social networks, we collected data from more than 500groups of reef manta rays over five years, in the Raja Ampat Regency of West Papua.We used generalized affiliation indices to isolate social preferences from non-socialassociations, the first study on elasmobranchs to use this method. Longer lastingsocial preferences were detected mostly between female rays. We detectedassortment of social relations by phenotype and variation in social strategies, with theoverall social network divided into two main communities. Overall network structurewas characteristic of a dynamic fission-fusion society, with differentiated relationshipslinked to strong fidelity to cleaning station sites. Our results suggest that fine-scaleconservation measures will be useful in protecting social groups of M. alfredi in theirnatural habitats, and that a more complete understanding of the social nature of mantarays will help predict population response
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Effect of carbon-fiber-reinforced polymer laminate configuration on the behavior of strengthened reinforced concrete beams
This paper presents test results of 18 small-scale reinforced concrete specimens of strengthened beams using carbon-fiber-reinforced polymer (CFRP) composites. The specimens were instrumented with strain gauges in a region where cracks in the concrete were preformed to monitor the variation of strains throughout testing. Results indicate that there can be a very large variation in the measured strains in the composites depending, not only on the location of the cracks, but also on the configuration used to bond the composites to the surface of the elements. The interface shear stresses generated at failure of the beams are compared with two existing analytical models. Additionally, the stress level in the composites was determined for all the strengthened specimens from the experimental data. The calculated stress in the composites reached between 20 and 43% of the CFRP rupture stress. The information presented in this paper provides information that can be used to validate or modify current design procedures of strengthened beams using composites
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Hysteretic Behavior of bridge columns with FRP-Jacketed lap splices designed for moderate ductility enhancement
This paper presents test results of six specimens representing older bridge columns with inadequate reinforcement detailing consisting of short lap splices at the base and widely spaced transverse reinforcement. Four of these specimens were rehabilitated using fiber-reinforced polymer (FRP) jackets of two different composite materials (carbon and aramid) to avoid premature failure of the lapped bars after a limited number of postyield cycles. The test results indicate that thin FRP jackets can be used to avoid failure of short lap splices at moderate displacement ductilities. Displacement capacities consistent with expected demands in regions of moderate or low seismicity were achieved after jacket retrofitting. The hysteretic behavior of rehabilitated columns was assessed with emphasizing issues related to variation of stiffness and damping ratio as a function of ductility demand for this class of columns. Equations that account for the effect of axial load level on estimates of effective stiffness and damping as a function of displacement ductility are proposed for this class of columns
Plasmonic ELISA for the detection of analytes at ultralow concentrations with the naked eye
This protocol describes a signal-generation mechanism for the naked-eye detection of analytes at low concentrations with ELISA. The key step is to generate solutions of desired tonality by growing gold nanoparticles with a particular state of aggregation. This is accomplished by linking the growth of gold nanoparticles with the biocatalytic cycle of the enzyme label. The protocol adapts a conventional ELISA procedure with catalase-labeled antibodies. The enzyme consumes hydrogen peroxide, and then gold (III) ions are added to generate gold nanoparticles. The concentration of hydrogen peroxide dictates the state of aggregation of gold nanoparticles. This allows for the naked-eye detection of analytes by observing the generation of blue- or red-colored gold nanoparticle solutions. When coupled with conventional ELISA, this signal-generation procedure allows for the naked-eye detection of analytes within 1 h