Environmental enrichment reduces signs of boredom in caged mink

Animals housed in impoverished cages are often labelled 'bored'. They have also been called 'apathetic' or 'depressed', particularly when profoundly inactive. However, these terms are rarely operationally defined and validated. As a negative state caused by under-stimulation, boredom should increase interest in stimuli of all kinds. Apathy (lack of interest), by contrast, should manifest as decreased interest in all stimuli, while anhedonia (loss of pleasure, a depressive symptom) should specifically decrease interest in normally rewarding stimuli. We tested the hypotheses that mink, a model carnivore, experience more boredom, depression-like apathy, or anhedonia in non-enriched (NE) cages than in complex, enriched (E) cages. We exposed 29 subjects (13 E, 16 NE) to ten stimuli categorized a priori as aversive (e.g. air puffs), rewarding (e.g. evoking chasing) or ambiguous/neutral (e.g. candles). Interest in stimuli was assessed via latencies to contact, contact durations, and durations oriented to stimuli. NE mink contacted all stimuli faster (P = 0.003) than E mink, and spent longer oriented to/in contact with them, albeit only significantly so for ambiguous ones (treatment*type P<0.013). With stimulus category removed from statistical models, interest in all stimuli was consistently higher among NE mink (P<0.0001 for all measures). NE mink also consumed more food rewards (P = 0.037). Finally, we investigated whether lying down while awake and stereotypic behaviour (both increased by NE housing) predicted these responses. Lying awake positively co-varied with certain measures of increased exploration. In contrast, stereotypic 'scrabbling' or locomotion (e.g. pacing) did not. Overall, NE mink showed no evidence of apathy or depression, but instead a heightened investigation of diverse stimuli consistent with boredom. This state was potentially indicated by spending much time lying still but awake (although this result requires replication). Boredom can thus be operationalized and assessed empirically in non-human animals. It can also be reduced by environmental enrichment

Can the electromyographic fatigue threshold be determined from superficial elbow flexor muscles during an isometric single-joint task?

The purpose of this study was to compare the electromyographic fatigue threshold (EMG) values determined simultaneously from superficial elbow flexor muscles during an isometric single-joint task. Eight subjects performed isometric elbow flexions at randomly ordered percentages of maximal voluntary contraction (20, 30, 40, 50 and 60%). During these bouts, electromyographic (EMG) activity was measured in the anterior head of Deltoïd, lateral head of Triceps brachii, Brachioradialis and both short and long head of Biceps brachii. For each subject and each muscle, the EMG amplitude data were plotted as function of time for the five submaximal bouts. The slope coefficient of the EMG amplitude versus time linear relationships were plotted against force level. EMG was determined as the y-intercept of this relationship and considered as valid only if the following criteria were met: (1) significant positive linear regression (P < 0.05) between force and slope coefficient, (2) an adjusted coefficient of determination for force versus slope coefficient relationship greater than 0.85, and (3) a standard error for the EMG below 5% of maximal voluntary contraction. The EMG could only be determined for one muscle (the long head of Biceps brachii) and only in three out of the eight subjects (mean value = 24.9 ± 1.1% of maximal voluntary contraction). The lack of EMG in most of the subjects (5/8) could be explained by putative compensations between elbow muscles which were indirectly observed in some subjects. In this way, EMG should be studied from a more simple movement i.e., ideally a movement implying mainly one muscle

Common input to different regions of biceps brachii long head

The purpose of the experiment was to compare the level of synchronization exhibited by pairs of motor units located within and between functionally distinct regions of the biceps brachii muscle. Pairs of single motor units were recorded from seven subjects using separate electrodes located in the lateral and medial aspects of the long head of biceps brachii. Participants were required to exert a combination of flexion and supination torques so that both motor units discharged at approximately 10 pps for >/=200 s and the level of motor unit synchronization could be quantified. When motor unit recordings were sufficiently stable at the completion of this synchrony task, a series of ramp contractions with multiple combinations of flexion and supination torques were performed to characterize the recruitment thresholds of the motor units. Common input strength (CIS) was significantly greater (P < 0.01) for the within-region pairs of motor units (0.28 extra sync. imps/s, n = 26) than for the between-region pairs (0.13 extra sync. imps/s, n = 18), but did not differ significantly for the 12 within-region pairs from the lateral head and 14 from the medial head (0.27 vs. 0.29 extra sync. imps/s; P = 0.83). Recruitment thresholds were measured for 33 motor units, but there was only a weak association between CIS and the respective recruitment patterns for motor unit pairs (n = 9). The present investigation provides evidence of a differential distribution of synaptic input across the biceps brachii motor neuron pool, but this appears to have minimal association with the recruitment patterns for individual motor units