NLRP3 Inflammasome: Key Mediator of Neuroinflammation in Murine Japanese Encephalitis

Background: Japanese Encephalitis virus (JEV) is a common cause of acute and epidemic viral encephalitis. JEV infection is associated with microglial activation resulting in the production of pro-inflammatory cytokines including Interleukin-1 b (IL-1b) and Interleukin-18 (IL-18). The Pattern Recognition Receptors (PRRs) and the underlying mechanism by which microglia identify the viral particle leading to the production of these cytokines is unknown. Methodology/Principal Findings: For our studies, we have used murine model of JEV infection as well as BV-2 mouse microglia cell line. In this study, we have identified a signalling pathway which leads to the activation of caspase-1 as the key enzyme responsible for the maturation of both IL-1b and IL-18 in NACHT, LRR and PYD domains-containing protein-3 (NLRP3) dependent manner. Depletion of NLRP3 results in the reduction of caspase-1 activity and subsequent production of these cytokines. Conclusion/Significance: Our results identify a mechanism mediated by Reactive Oxygen Species (ROS) production and potassium efflux as the two danger signals that link JEV infection to caspase-1 activation resulting in subsequent IL-1b an

Nutritional survey of kinnow orchards soil series at Rawatsar and Fatehgarh of Hanumangarh district of arid Rajasthan

The present study was conducted to evaluate the fertility status of kinnow orchards at Rawatsar and Fatehgarh soil series of Hanumangarh district in irrigated area of arid Rajasthan. All kinnow orchards soils were found saline in nature, EC2 of all the orchard soil samples was normal. The calcium carbonate content in soils showed increasing trend with depth, low in organic carbon and available N, low to medium in available P2 O5, medium to high in available K2O content, majority of samples were found high in available Fe, available Mn and low to sufficient in available Cu and low in available Zn content. Leaf N content were found low in kinnow orchards, majority of leaf samples found medium to optimum in leaf P, Fe, and Mn content, whereas leaf K content and Zn content were found low to medium in kinnow orchards. The leaves Cu content were found optimum range in kinnow orchards. Fruit juice N, Fe and Mn content were found low to optimum, whereas fruit juice P, K and Cu content were found optimum and Zn content low at Rawatsar and Fatehgarh soil series of Hanumangarh district

Nutritional survey of kinnow orchards soil series at Rawatsar and Fatehgarh of Hanumangarh district of arid Rajasthan

The present study was conducted to evaluate the fertility status of kinnow orchards at Rawatsar and Fatehgarh soil series of Hanumangarh district in irrigated area of arid Rajasthan. All kinnow orchards soils were found saline in nature, EC2 of all the orchard soil samples was normal. The calcium carbonate content in soils showed increasing trend with depth, low in organic carbon and available N, low to medium in available P2 O5, medium to high in available K2O content, majority of samples were found high in available Fe, available Mn and low to sufficient in available Cu and low in available Zn content. Leaf N content were found low in kinnow orchards, majority of leaf samples found medium to optimum in leaf P, Fe, and Mn content, whereas leaf K content and Zn content were found low to medium in kinnow orchards. The leaves Cu content were found optimum range in kinnow orchards. Fruit juice N, Fe and Mn content were found low to optimum, whereas fruit juice P, K and Cu content were found optimum and Zn content low at Rawatsar and Fatehgarh soil series of Hanumangarh district