8 research outputs found
Development of a cellâfree protein synthesis protocol to rapidly screen Lâasparaginase
Holistic engineering of cell-free systems through proteome-reprogramming synthetic circuits
Cell-free protein synthesis from genomically recoded bacteria enables multisite incorporation of noncanonical amino acids
An antimicrobial peptide that inhibits translation by trapping release factors on the ribosome
Synthetic Biology at the Hand of Cell-Free Systems
International audienceCell-free systems are emerging as membraneless transcriptional and translational tools to study biology and for prototyping, characterization, and engineering novel biological systems. Cell-free systems allow in vitro transcription and translation using the cellular machinery prepared from a variety of prokaryotic and eukaryotic cells. These non-GMO tools enable rapid and high-throughput characterization by their rapid gene expression, simple building of large combinatorial libraries, easier cloning, lower noise, less susceptibility to toxicity, and high tunability. In this chapter, different types of cell-free systems, different techniques to obtain them, and their applications in synthetic biology and bioengineering are presented