Levamisole promotes an adjuvant effect on the immunity of pacu (Piaractus mesopotamicus) when immunized with Aeromonas hydrophila, even when provided in the diet

AbstractDietary immunomodulators can help to defend against disease, but their dosage and time of administration are not always clear in aquaculture. In this study, we analyzed the amount of dietary levamisole required to induce an adjuvant effect. Because immunostimulants and immunization can improve the fish immune system, this study evaluated the effects of dietary levamisole administration (at 0, 125, 250 and 500mg/kg diet) and immunization with A. hydrophila (1×109 CFU) on the acquired and innate immune systems of pacu (mean initial weight 176.03±15.73g). The results showed that levamisole can act as an adjuvant for vaccination, even when administered through the diet. Immunization and levamisole administration increased the antibody titer, serum bactericidal activity, and hematocrit as well as the numbers of red blood cells, leukocytes and thrombocytes in pacu (P<0.05). However, the lysozyme activity; leukocyte respiratory activity; total protein, albumin, and globulin levels; A:G index; corpuscular hemoglobin volume; and other white blood cells showed no differences when compared with the control fish. As the immunostimulant improved, certain parameters of the acquired and innate immune systems of pacu increased, and the administration of levamisole in the diet (125 or 250mg/kg) for seven days may be used to promote adjuvant effects during immunization with A. hydrophila and thus constitutes a feed protocol strategy to be used prior to immunization

Introduction to Quantum-Gravity Phenomenology

After a brief review of the first phase of development of Quantum-Gravity Phenomenology, I argue that this research line is now ready to enter a more advanced phase: while at first it was legitimate to resort to heuristic order-of-magnitude estimates, which were sufficient to establish that sensitivity to Planck-scale effects can be achieved, we should now rely on detailed analyses of some reference test theories. I illustrate this point in the specific example of studies of Planck-scale modifications of the energy/momentum dispersion relation, for which I consider two test theories. Both the photon-stability analyses and the Crab-nebula synchrotron-radiation analyses, which had raised high hopes of ``beyond-Plankian'' experimental bounds, turn out to be rather ineffective in constraining the two test theories. Examples of analyses which can provide constraints of rather wide applicability are the so-called ``time-of-flight analyses'', in the context of observations of gamma-ray bursts, and the analyses of the cosmic-ray spectrum near the GZK scale.Comment: 46 pages, LaTex. Based on lectures given at the 40th Karpacz Winter School in Theoretical Physic

Corrections to flat-space particle dynamics arising from space granularity

The construction of effective Hamiltonians describing corrections to flat space particle dynamics arising from the granularity of space at very short distances is discussed in the framework of an heuristic approach to the semiclassical limit of loop quantum gravity. After some general motivation of the subject, a brief non-specialist introduction to the basic tools employed in the loop approach is presented. The heuristical semiclassical limit is subsequently defined and the application to the case of photons and spin 1/2 fermions is described. The resulting modified Maxwell and Dirac Hamiltonians, leading in particular to Planck scale corrections in the energy-momentum relations, are presented. Alternative interpretations of the results and their limitations, together with other approaches are briefly discussed along the text. Three topics related to the above methods are reviewed: (1) The determination of bounds to the Lorentz violating parameters in the fermionic sector, obtained from clock comparison experiments.(2) The calculation of radiative corrections in preferred frames associated to space granularity in the framework of a Yukawa model for the interactions and (3) The calculation of synchrotron radiation in the framework of the Myers-Pospelov effective theories describing Lorentz invariance violations, as well as a generalized approach to radiation in Planck scale modified electrodynamics. The above exploratory results show that quantum gravity phenomenology provides observational guidance in the construction of quantum gravity theories and opens up the possibility of probing Planck scale physics.Comment: 49 pages, 6 figures and 4 tables. Extended version of the talk given at the 339-th WE-Heraeus-Seminar: Special Relativity, will it survive the next 100 years?, Potsdam, february 200

The Sudbury Neutrino Observatory

The Sudbury Neutrino Observatory is a second generation water Cherenkov detector designed to determine whether the currently observed solar neutrino deficit is a result of neutrino oscillations. The detector is unique in its use of D2O as a detection medium, permitting it to make a solar model-independent test of the neutrino oscillation hypothesis by comparison of the charged- and neutral-current interaction rates. In this paper the physical properties, construction, and preliminary operation of the Sudbury Neutrino Observatory are described. Data and predicted operating parameters are provided whenever possible.Comment: 58 pages, 12 figures, submitted to Nucl. Inst. Meth. Uses elsart and epsf style files. For additional information about SNO see http://www.sno.phy.queensu.ca . This version has some new reference

Serum bactericidal activity as indicator of innate immunity in pacu Piaractus mesopotamicus (Holmberg, 1887)

The immune system of teleost fish has mechanisms responsible for the defense against bacteria through protective proteins in several tissues. The protein action can be evaluated by serum bactericidal activity and this is an important tool to analyze the immune system. Pacu, Piaractus mesopotamicus, is one of the most important fish in national aquaculture. However there is a lack of studies on its immune responses. In order to standardize and assess the accuracy of the serum bactericidal activity assay, fish were briefly challenged with Aeromonas hydrophila and sampled one week after the challenge. The bacterial infection increased the concentration of protective proteins, resulting in a decrease of colony-forming unit values expressed as well as an enhanced serum bactericidal activity. The protocol showed a reliable assay, appropriate to determine the serum bactericidal activity of pacu in the present experimental conditions.O sistema imune de peixes teleósteos tem mecanismos responsáveis pela defesa contra bactérias e atua através de proteínas presentes em diversos tecidos. A ação destas proteínas pode ser avaliada pela atividade bactericida do soro, sendo esta uma importante ferramenta para analisar o sistema imune. O pacu, Piaractus mesopotamicus, é um peixe nativo muito importante para aquicultura nacional, entretanto há pouco conhecimento sobre o funcionamento de seu sistema imune. Assim foi realizado experimento para padronizar e avaliar a eficiência do ensaio de atividade bactericida. Resumidamente, peixes foram desafiados por Aeromonas hydrophila e amostradas uma semana após o desafio. A infecção bacteriana promoveu um aumento na concentração de proteínas protetoras, resultando em diminuição dos valores de unidades formadoras de colônias ou expressos também como aumento da atividade bactericida do soro. O protocolo se mostrou confiável, sendo apropriado para determinar a atividade bactericida do soro de pacu nas condições experimentais.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES