Porcine ovarian biometry, oocyte retrieval and quality of oocytes under different seasons in north east hill region

The present study was conducted to evaluate the effect of different seasons on porcine ovarian biometry and centred, oocyte retrieval and quality of oocytes in north east hill region of India. Porcine ovaries (1886) were collected over a period of 12 months from local abattoirs. The morphological studies revealed mulberry shaped multi- lobulated ovaries with an average weight of 4.435 ± 0.622 The average number of GF and CL were significantly higher in winter than monsoon) but not significantly different from pre-monsoon/summer. The number of CA was significantly higher in winter than monsoon and summer. The number of recovered oocyte from the visible follicles in oocyte collection medium had significantly higher number in winter than other two seasons. The oocyte percentage of grade-I, grade-II, grade-III and grade-IV were found to be 24, 36, 23 and 17%, respectively. The culturable oocytes were significantly higher during winter than summer and monsoon seasons. The season had significant effect on quality and quantity of porcine ovaries which could be well utilized in-vitro maturation and fertilization by the research in field of reproduction and biotechnology

Detection of foot-and-mouth disease virus type O in recovered as well as healthy cattle to study carrier status in Assam

200-206Foot and mouth disease (FMD), one of the most contagious diseases of animals, affects different host species including wild animals. Asymptomatic FMD recovered animals may remain as carrier, which may be threat to other healthy animals. Hence, it is necessary to monitor the carrier status of the FMD recovered animals to effectively prevent further spread of the disease. Out of all the seven serotypes of FMD, O serotype is most commonly found in livestock. Therefore, in the present study, we chose to detect serotype ‘O’ in oropharyngeal fluid (OP) and to quantify cytokines, viz. IL-1α, IL-1β and IL-2. A total of 30 OP fluids and 30 blood samples were collected from 10 animals (1 in-contact healthy animal) for 3 months post infection. FMD O serotype could be detected in all the animals (100%). The RQ values were found to be 0.014 to 63.118 and 0.162 to 46.889 for IL-1α and IL-1β genes respectively, while insignificant RQ values were obtained for IL-2. In the second and third months, two animals showed down regulation for IL-1α gene, while IL-1β and IL-2 genes were down regulated in 7 animals and in all 10 animals, respectively for all the three months

Detection of foot-and-mouth disease virus type O in recovered as well as healthy cattle to study carrier status in Assam

Foot and mouth disease (FMD), one of the most contagious diseases of animals, affects different host species including wild animals. Asymptomatic FMD recovered animals may remain as carrier, which may be threat to other healthy animals. Hence, it is necessary to monitor the carrier status of the FMD recovered animals to effectively prevent further spread of the disease. Out of all the seven serotypes of FMD, O serotype is most commonly found in livestock. Therefore, in the present study, we chose to detect serotype ‘O’ in oropharyngeal fluid (OP) and to quantify cytokines, viz. IL-1α, IL-1β and IL-2. A total of 30 OP fluids and 30 blood samples were collected from 10 animals (1 in-contact healthy animal) for 3 months post infection. FMD O serotype could be detected in all the animals (100%). The RQ values were found to be 0.014 to 63.118 and 0.162 to 46.889 for IL-1α and IL-1β genes respectively, while insignificant RQ values were obtained for IL-2. In the second and third months, two animals showed down regulation for IL-1α gene, while IL-1β and IL-2 genes were down regulated in 7 animals and in all 10 animals, respectively for all the three months