A novel 3-hydroxypropionic acid-inducible promoter regulated by the LysR-type transcriptional activator protein MmsR of Pseudomonas denitrificans

MmsR (33.3 kDa) is a putative LysR-type transcriptional activator of Pseudomonas denitrificans. With the help of 3-hydroxypropionic acid (3-HP), an important platform chemical, MmsR positively regulates the expression of mmsA, which encodes methylmalonylsemialdehyde dehydrogenase, the enzyme involved in valine degradation. In the present study, the cellular function of MmsR and its binding to the regulatory DNA sequence of mmsA expression were investigated both in vivo and in vitro. Transcription of the mmsA was enhanced >140-fold in the presence of 3-HP. In the MmsR-responsive promoter region, two operators showing dyad symmetry, designated O-1 and O-2 and centered at the -79 and -28 positions, respectively, were present upstream of the mmsA transcription start site. An electrophoretic mobility shift assay indicated that MmsR binds to both operator sites for transcription activation, probably in cooperative manner. When either O-1 or O-2 or both regions were mutated, the inducibility by the MmsR-3-HP complex was significantly reduced or completely removed, indicating that both sites are required for transcription activation. A 3-HP sensor was developed by connecting the activation of MmsR to a green fluorescent readout. A more than 50-fold induction by 25 mM 3-HP was observed

Geological observations in the southern West Greenland basement from Ameralik to Frederikshåb Isblink in 2008

In 2008, the Geological Survey of Denmark and Greenland began a project in collaboration with the Bureau of Minerals and Petroleum of Greenland with the aim to publish a web-based, seamless digital map of the Precambrian bedrock between 61°30´ and 64°N in southern West Greenland. Such a map will be helpful for the mineral exploration industry and for basic research. Producing an updated digital map requires additional field work revisiting key localities to collect samples for geochemistry, geochronology and metamorphic petrology. The new data will help us to test and refine existing models and improve general understanding of the geological evolution of the area. Here we summarise some results from the 2008 field activities between Ame - ralik in the north and Frederikshåb Isblink in the south (Fig. 1). The area was mapped in the 1960s and 1970s, and although the 1:100 000-scale maps are of excellent quality, they do not include more recent developments in geochronology, thermobarometry and geochemistry. A notable exception is the Fiskenæsset complex (Fig. 1), which has received considerable attention after it was first mapped (Ellitsgaard-Rasmussen & Mouritzen 1954; Windley et al. 1973; Windley & Smith, 1974; Myers 1985). New tectonic models have been developed since the original 1:100 000 maps were produced, and the tectonic evolution has been com - monly ex plained in terms of terrane accretion (Friend et al. 1996)