564 research outputs found
Liquid chromatography-tandem mass spectrometry for the simultaneous quantitation of ceftriaxone, metronidazole and hydroxymetronidazole in plasma from seriously ill, severely malnourished children.
We have developed and validated a novel, sensitive, selective and reproducible reversed-phase high-performance liquid chromatography method coupled with electrospray ionization mass spectrometry (HPLC-ESI-MS/MS) for the simultaneous quantitation of ceftriaxone (CEF), metronidazole (MET) and hydroxymetronidazole (MET-OH) from only 50 µL of human plasma, and unbound CEF from 25 µL plasma ultra-filtrate to evaluate the effect of protein binding. Cefuroxime axetil (CEFU) was used as an internal standard (IS). The analytes were extracted by a protein precipitation procedure with acetonitrile and separated on a reversed-phase Polaris 5 C18-Analytical column using a mobile phase composed of acetonitrile containing 0.1% (v/v) formic acid and 10 mM aqueous ammonium formate pH 2.5, delivered at a flow-rate of 300 µL/min. Multiple reaction monitoring was performed in the positive ion mode using the transitionsm/z555.1→m/z396.0 (CEF),m/z172.2→m/z128.2 (MET),m/z188.0→m/z125.9 (MET-OH) andm/z528.1→m/z364.0 (CEFU) to quantify the drugs. Calibration curves in spiked plasma and ultra-filtrate were linear (r2≥ 0.9948) from 0.4-300 µg/mL for CEF, 0.05-50 µg/mL for MET and 0.02 - 30 µg/mL for MET-OH. The intra- and inter- assay precisions were less than 9% and the mean extraction recoveries were 94.0% (CEF), 98.2% (MET), 99.6% (MET-OH) and 104.6% (CEF in ultra-filtrate); the recoveries for the IS were 93.8% (in plasma) and 97.6% (in ultra-filtrate). The validated method was successfully applied to a pharmacokinetic study of CEF, MET and MET-OH in hospitalized children with complicated severe acute malnutrition following an oral administration of MET and intravenous administration of CEF over the course of 72 hours
Geometrical dependence of low frequency noise in superconducting flux qubits
A general method for directly measuring the low-frequency flux noise (below
10 Hz) in compound Josephson junction superconducting flux qubits has been used
to study a series of 85 devices of varying design. The variation in flux noise
across sets of qubits with identical designs was observed to be small. However,
the levels of flux noise systematically varied between qubit designs with
strong dependence upon qubit wiring length and wiring width. Furthermore,
qubits fabricated above a superconducting ground plane yielded lower noise than
qubits without such a layer. These results support the hypothesis that
localized magnetic impurities in the vicinity of the qubit wiring are a key
source of low frequency flux noise in superconducting devices.Comment: 5 pages, 5 figure
Probing Noise in Flux Qubits via Macroscopic Resonant Tunneling
Macroscopic resonant tunneling between the two lowest lying states of a
bistable RF-SQUID is used to characterize noise in a flux qubit. Measurements
of the incoherent decay rate as a function of flux bias revealed a Gaussian
shaped profile that is not peaked at the resonance point, but is shifted to a
bias at which the initial well is higher than the target well. The r.m.s.
amplitude of the noise, which is proportional to the decoherence rate 1/T_2^*,
was observed to be weakly dependent on temperature below 70 mK. Analysis of
these results indicates that the dominant source of low frequency (1/f) flux
noise in this device is a quantum mechanical environment in thermal
equilibrium.Comment: 4 pages 4 figure
Profile: The Kilifi Health and Demographic Surveillance System (KHDSS).
The Kilifi Health and Demographic Surveillance System (KHDSS), located on the Indian Ocean coast of Kenya, was established in 2000 as a record of births, pregnancies, migration events and deaths and is maintained by 4-monthly household visits. The study area was selected to capture the majority of patients admitted to Kilifi District Hospital. The KHDSS has 260 000 residents and the hospital admits 4400 paediatric patients and 3400 adult patients per year. At the hospital, morbidity events are linked in real time by a computer search of the population register. Linked surveillance was extended to KHDSS vaccine clinics in 2008. KHDSS data have been used to define the incidence of hospital presentation with childhood infectious diseases (e.g. rotavirus diarrhoea, pneumococcal disease), to test the association between genetic risk factors (e.g. thalassaemia and sickle cell disease) and infectious diseases, to define the community prevalence of chronic diseases (e.g. epilepsy), to evaluate access to health care and to calculate the operational effectiveness of major public health interventions (e.g. conjugate Haemophilus influenzae type b vaccine). Rapport with residents is maintained through an active programme of community engagement. A system of collaborative engagement exists for sharing data on survival, morbidity, socio-economic status and vaccine coverage
Sign- and magnitude-tunable coupler for superconducting flux qubits
We experimentally confirm the functionality of a coupling element for
flux-based superconducting qubits, with a coupling strength whose sign and
magnitude can be tuned {\it in situ}. To measure the effective , the
groundstate of a coupled two-qubit system has been mapped as a function of the
local magnetic fields applied to each qubit. The state of the system is
determined by directly reading out the individual qubits while tunneling is
suppressed. These measurements demonstrate that can be tuned from
antiferromagnetic through zero to ferromagnetic.Comment: Updated text and figure
Entanglement in a quantum annealing processor
Entanglement lies at the core of quantum algorithms designed to solve
problems that are intractable by classical approaches. One such algorithm,
quantum annealing (QA), provides a promising path to a practical quantum
processor. We have built a series of scalable QA processors consisting of
networks of manufactured interacting spins (qubits). Here, we use qubit
tunneling spectroscopy to measure the energy eigenspectrum of two- and
eight-qubit systems within one such processor, demonstrating quantum coherence
in these systems. We present experimental evidence that, during a critical
portion of QA, the qubits become entangled and that entanglement persists even
as these systems reach equilibrium with a thermal environment. Our results
provide an encouraging sign that QA is a viable technology for large-scale
quantum computing.Comment: 13 pages, 8 figures, contact corresponding author for Supplementary
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