Developing molecular genetic markers for grape breeding; using polymerase chain reaction procedures

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Genomic DNA fingerprinting of Vitis vinifera by the use of multi-loci probes

Charakterisierung genomischer DNA von Vitis vinifera mit Hilfe von ,,Multi-loci-Sonden"Durch Southern-Transfer von Restriktionsfragmenten genomischer DNA aus Vitis vinifera ließen sich Unterschiede zwischen verschiedenen Rebsorten aufzeigen; für die Hybridisierung wurden Sonden aus M 13-DNA und Human-33.6-Minisatelliten-DNA verwendet. Die Restriktionsenzyme Hinf I und Hae III waren für die Bestimmung sortenspezifischer DNA-Muster gleichermaßen geeignet. Die M 13-Sonde identifizierte in Verbindung mit beiden Enzymen etwa 3mal soviele Banden und zeigte viel mehr Abweichungen zwischen den Sorten auf als die Human-Minisatelliten-Sonde. Das Differenzierungsverfahren kann bei der Rebe zur Analyse der Abstammung, zur Definition von Merkmalen bei sortengeschützten Reben sowie für Züchtungsprogramme eingesetzt werden, sobald Korrelationen mit wirtschaftlich wichtigen Eigenschaften gesichert sind.

Mn 2+ reduces Y z + in manganese-depleted Photosystem II preparations

Manganese in the oxygen-evolving complex is a physiological electron donor to Photosystem II. PS II depleted of manganese may oxidize exogenous reductants including benzidine and Mn 2+ . Using flash photolysis with electron spin resonance detection, we examined the room-temperature reaction kinetics of these reductants with Y z + , the tyrosine radical formed in PS II membranes under illumination. Kinetics were measured with membranes that did or did not contain the 33 kDa extrinsic polypeptide of PS II, whose presence had no effect on the reaction kinetics with either reductant. The rate of Y z + reduction by benzidine was a linear function of benzidine concentration. The rate of Y z + reduction by Mn 2+ at pH 6 increased linearly at low Mn 2+ concentrations and reached a maximum at the Mn 2+ concentrations equal to several times the reaction center concentration. The rate was inhibited by K + , Ca 2+ and Mg 2+ . These data are described by a model in which negative charge on the membrane causes a local increase in the cation concentration. The rate of Y z + reduction at pH 7.5 was biphasic with a fast 400 μs phase that suggests binding of Mn 2+ near Y z + at a site that may be one of the native manganese binding sites.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/43534/1/11120_2004_Article_BF00048306.pd

Changing root and shoot architecture with the rolA gene from Agrobacterium rhizogenes: Interactions with gibberellic acid and polyamine metabolism

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