Local inflammation, lethality and cytokine release in mice injected with Bothrops atrox venom.

We have provided evidence that: (a) lethality of mice to crude Bothrops venom varies according the isogenic strain (A/J > C57Bl/6 > A/Sn > BALB/c > C3H/HePas > DBA/2 > C3H/He); (b)BALB/c mice (LD50=100.0 microg) were injected i.p. with 50 microg of venom produced IL-6, IL-10, INF-gamma, TNF-alpha and NO in the serum. In vitro the cells from the mice injected and challenged with the venom only released IL-10 while peritoneal macrophages released IL-10, INF-gamma and less amounts of IL-6; (c) establishment of local inflammation and necrosis induced by the venom, coincides with the peaks of TNF-alpha, IFN-gamma and NO and the damage was neutralized when the venom was incubated with a monoclonal antibody against a 60 kDa haemorrhagic factor. These results suggest that susceptibility to Bothrops atrox venom is genetically dependent but MHC independent; that IL-6, IL-10, TNF-alpha, IFN-gamma and NO can be involved in the mediation of tissue damage; and that the major venom component inducers of the lesions are haemorrhagins

Mutucas (Diptera: Tabanidae) do Pantanal: abundância relativa e sazonalidade na sub-região da Nhecolândia.

De junho/92 a maio/94, capturas de mutucas foram realizadas mensalmente em eqüino e utilizando armadilhas do tipo ?canopy?, na fazenda Nhumirim, subregião da Nhecolândia, Pantanal sul-mato-grossense. Capturas no eqüino foram realizadas do crepúsculo matutino ao vespertino, com o auxílio de redes entomológicas, em ambientes de campo e cerradão (1 dia/ambiente/mês), durante o primeiro ano do estudo. Capturas com armadilhas foram realizadas nos mesmos ambientes (10 dias/ambiente/mês), por dois anos. Foram capturadas 6.274 mutucas ao longo do estudo, pertencentes a 25 espécies, 13 gêneros e 3 subfamílias. A espécies mais abundantes foram Tabanus importunus (44,04%), Tabanus occidentalis (15,95%), Tabanus claripennis (9,98%) e Lepiselaga crassipes (7,60%). Apesar do menor esforço de captura, as coletas no eqüino foram mais eficientes que as realizadas com armadilhas, totalizando 3.442 (54,9%) e 2.832 (45,1%) mutucas, respectivamente. Picos populacionais foram observados próximos ao início do período chuvoso, geralmente entre setembro e novembro (primavera). Entretanto, as mutucas foram relativamente abundantes também durante parte do verão. Os resultados obtidos nestes estudos indicam que mutucas são mais abundantes durante a época chuvosa, particularmente na primavera, considerada a época de maior risco de transmissão mecânica de patógenos por estes vetores.bitstream/item/37402/1/BP48.pd

Group Theory analysis of phonons in two-dimensional Transition Metal Dichalcogenides

Transition metal dichalcogenides (TMDCs) have emerged as a new two dimensional materials field since the monolayer and few-layer limits show different properties when compared to each other and to their respective bulk materials. For example, in some cases when the bulk material is exfoliated down to a monolayer, an indirect-to-direct band gap in the visible range is observed. The number of layers $N$ ($N$ even or odd) drives changes in space group symmetry that are reflected in the optical properties. The understanding of the space group symmetry as a function of the number of layers is therefore important for the correct interpretation of the experimental data. Here we present a thorough group theory study of the symmetry aspects relevant to optical and spectroscopic analysis, for the most common polytypes of TMDCs, i.e. $2Ha$, $2Hc$ and $1T$, as a function of the number of layers. Real space symmetries, the group of the wave vectors, the relevance of inversion symmetry, irreducible representations of the vibrational modes, optical selection rules and Raman tensors are discussed.Comment: 32 pages, 4 figure

Pecuária de corte e a conservação do Pantanal.

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A VEL3 histone deacetylase complex establishes a maternal epigenetic state controlling progeny seed dormancy

Mother plants play an important role in the control of dormancy and dispersal characters of their progeny. In Arabidopsis seed dormancy is imposed by the embryo-surrounding tissues of the endosperm and seed coat. Here we show that VERNALIZATION5/VIN3-LIKE 3 (VEL3) maintains maternal control over progeny seed dormancy. Acting early in endosperm development, it establishes an epigenetic state that primes the depth of primary seed dormancy that will be reached later during seed maturation. VEL3 relocates MSI1 to the nucleolus and associates with other components of the histone deacetylase complex (HDAC). Furthermore VEL3 preferentially associates with pericentromeric chromatin and is required for deacetylation and H3K27me3 deposition in the central cell. Interestingly, the epigenetic state established by the maternal VEL3 is retained in mature seeds, and controls seed dormancy in part through the repression of programmed cell death-associated gene ORE1. Our data demonstrates a novel mechanism by which maternal control of progeny seed physiology persists post-shedding, maintaining parental control of seed behaviour