Commissioning and improvements of the instrumentation and launch of the scientific exploitation of OARPAF, the Regional Astronomical Observatory of the Antola Park

The OARPAF telescope is an 80-cm-diameter optical telescope installed in the Antola Mount Regional Reserve, in Northern Italy. We present the results of the characterization of the site, as well as developments and interventions that have been implemented, with the goal of exploiting the facility for scientific and educational purposes. During the characterization of the site, an average background brightness of 22.40mAB (B filter) to 21.14mAB (I) per arcsecond squared, and a 1.5″ to 3.0″ seeing, have been measured. An estimate of the magnitude zero points for photometry is also reported. The material under commissioning includes three CCD detectors for which we provide the linearity range, gain, and dark current; a 31-orders échelle spectrograph with R ∼ 8500 to 15,000 and a dispersion of n = 1.39 × 10 − 6 px − 1λ + 1.45 × 10 − 4 nm / px, where λ is expressed in nm. The scientific and outreach potential of the facility is proven in different science cases, such as exoplanetary transits and active galactic nuclei variability. The determination of time delays of gravitationally lensed quasars, the microlensing phenomenon, and the tracking and the study of asteroids are also discussed as prospective science cases

CAPS: A New Method for the Identification of Different Surface Displacements in Landslide and Subsidence Environments through Correlation Analysis on Persistent Scatterers Time-Series from PSI

Persistent Scatterer Interferometry (PSI) is one of the most powerful tools for identifying and monitoring areas exposed to surface deformations such as landslides or subsidence. In this work, we propose a new method that we named CAPS (Correlation Analysis on Persistent Scatterers), to extend the capability of PSI in recognizing and characterising areas influenced by complex ground deformations and differential motions. CAPS must be applied to both ascending and descending orbits separately and comprises three major steps: (i) calculating the cross-correlation matrix on detrended PS time-series; (ii) extracting PS pairs with similarity greater than a given threshold; (iii) grouping PS in families by sorting and classification. Thus, in both orbits, PS Families identify groups of PS with similar movements. This allows distinguishing sectors characterised by different displacements over time even in areas with similar LOS (Line of Sight) velocities. As test sites, we considered four different known geological scenarios: two representing landslide environments (Santo Stefano d’Aveto and Arzeno, both in Liguria, NW Italy) and two subsidence environments (Rome and Venice, urban and surrounding areas). This method proved to be versatile, applicable to different geological situations and at different scales of observation, for recognizing both regional and local differential deformations

CAPS: A New Method for the Identification of Different Surface Displacements in Landslide and Subsidence Environments through Correlation Analysis on Persistent Scatterers Time-Series from PSI

Persistent Scatterer Interferometry (PSI) is one of the most powerful tools for identifying and monitoring areas exposed to surface deformations such as landslides or subsidence. In this work, we propose a new method that we named CAPS (Correlation Analysis on Persistent Scatterers), to extend the capability of PSI in recognizing and characterising areas influenced by complex ground deformations and differential motions. CAPS must be applied to both ascending and descending orbits separately and comprises three major steps: (i) calculating the cross-correlation matrix on detrended PS time-series; (ii) extracting PS pairs with similarity greater than a given threshold; (iii) grouping PS in families by sorting and classification. Thus, in both orbits, PS Families identify groups of PS with similar movements. This allows distinguishing sectors characterised by different displacements over time even in areas with similar LOS (Line of Sight) velocities. As test sites, we considered four different known geological scenarios: two representing landslide environments (Santo Stefano d’Aveto and Arzeno, both in Liguria, NW Italy) and two subsidence environments (Rome and Venice, urban and surrounding areas). This method proved to be versatile, applicable to different geological situations and at different scales of observation, for recognizing both regional and local differential deformations

Persistent Scatterer Interferometry and Statistical Analysis of Time-Series for Landslide Monitoring: Application to Santo Stefano d’Aveto (Liguria, NW Italy)

Landslides are a major threat for population and urban areas. Persistent Scatterer Interferometry (PSI) is a powerful tool for identifying landslides and monitoring their evolution over long periods and has proven to be very useful especially in urban areas, where a sufficient number of PS can be generated. In this study, we applied PS interferometry to investigate the landslide affecting Santo Stefano d’Aveto (Liguria, NW Italy) by integrating classic interferometric techniques with cross-correlation analysis of PS time-series and with geological and geotechnical field information. We used open-source software and packages to process Synthetic Aperture Radar (SAR) images from the Copernicus Sentinel-1A satellite for both ascending and descending orbits over the period 2015–2021 and calculate both the vertical motion and the E-W horizontal displacement. By computing the cross-correlation of the PS time-series, we identified three families of PS with a similarity greater than 0.70. The cross-correlation analysis allowed subdividing the landslide in different sectors, each of which is characterized by a specific type of movement. The geological meaning of this subdivision is still a matter of discussion but it is presumably driven by the geomorphological setting of the area and by the regional tectonics

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)

In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field