81 research outputs found
Analysis on Isotropic and Anisotropic Samples of Polypropylene/Polyethyleneterephthalate Blend/Graphene Nanoplatelets Nanocomposites: Effects of a Rubbery Compatibilizer
Over the past few years, polymer nanocomposites have garnered a significant amount of interest from both the scientific community and industry due to their remarkable versatility and wide range of potential uses in various fields, including automotive, electronics, medicine, textiles and environmental applications. In this regard, this study focuses on the influence of a compatibilizer rubber on a nanocomposite incorporating graphene nanoparticles (GNPs), with a polymer matrix based on a blend of polypropylene (PP) and polyethylene terephthalate (PET). This effect has been investigated on both isotropic samples and on anisotropic/spun fiber samples. The influence of the compatibilizer rubber on morphological, rheological and mechanical properties was analysed and discussed. Mechanical and morphological properties were evaluated on both isotropic samples obtained by compression moulding and melt-spun fibers. The addition of the rubbery compatibilizer increased the viscosity, improving interfacial adhesion, and the same effect was observed for the melt strength and breaking stretching ratios. Mechanical properties, including the elastic modulus, tensile strength and elongation at break, improved in both types of samples but more significantly in the fibers. These improvements were attributed to the orientation of the matrix, the formation of PET microfibrils, and the reduction in the size of graphene nanoparticles due to the action of the elongational flow. This reduction, facilitated by the elongation flow and the action of the compatibilizer, improved matrix-nanofiller adhesion due to the increased contact area between the two polymeric phases and between the filler and matrix. Finally, a transition from brittle to ductile behaviour was observed, particularly in the system with the compatibilizer, attributed to defect reduction and improved stress transmission
An optimized procedure for preparation of conditioned medium from Wharton’s jelly mesenchymal stromal cells isolated from umbilical cord
Cell-free therapy based on conditioned medium derived from mesenchymal stromal cells (MSCs) has gained attention in the field of protective and regenerative medicine. However, the exact composition and properties of MSC-derived conditioned media can vary greatly depending on multiple parameters, which hamper standardization. In this study, we have optimized a procedure for preparation of conditioned medium starting from efficient isolation, propagation and characterization of MSCs from human umbilical cord, using a culture medium supplemented with human platelet lysate as an alternative source to fetal bovine serum. Our procedure successfully maximizes the yield of viable MSCs that maintain canonical key features. Importantly, under these conditions, the compositional profile and biological effects elicited by the conditioned medium preparations derived from these MSC populations do not depend on donor individuality. Moreover, approximately 120 L of conditioned medium could be obtained from a single umbilical cord, which provides a suitable framework to produce industrial amounts of toxic-free conditioned medium with predictable composition
Throughput and energy efficiency in IEEE 802.11 WLANs: friends or foes?
Proceedings of: 6th International ICST Conference on Heterogeneous Networking for Quality, Reliability, Security and Robustness, QShine 2009
and
3rd International Workshop on Advanced Architectures and Algorithms for Internet Delivery and Applications, AAA-IDEA 2009, Las Palmas, Gran Canaria, November 23-25, 2009Understanding and optimizing the energy consumption of
wireless devices is critical to maximize network lifetime and to provide
guidelines for the design of new protocols and interfaces. In this work we
first provide an accurate analysis of the energy performance of an IEEE
802.11 WLAN, and then we derive the configuration to maximize it. We
also analyze the impact of the energy configuration of the device on the
throughput performance, and discuss in which circumstances throughput
and energy efficiency can be both maximized and where they constitute
different challenges.European Community's Seventh Framework ProgramPublicad
The sea urchin sns5 chromatin insulator settles a gene therapy vector into an independent domain of expression in the vertebrate genome
One of the critical aspects of introducing a transgene into the eukaryotic genome is the great variability of gene expression due to position effects (1). Chromatin-dependent repressive states could be overcome by incorporation in the transgene of chromatin insulators, functioning to establish and delimit domains of expression. We have previously demonstrated that the sea urchin sns5 DNA element has the typical features of an insulator: by acting as enhancer blocker, it shields promoters from neighboring regulatory elements, and by acting as barrier it buffers a transgene from the propagation of condensed chromatin (2,3).
We have investigated the use of sns5 in the field of gene therapy. Our preliminary studies shown that the inclusion of sns5 in \uf0e3-retroviral vectors allows position-independent expression in erythroid cells (4). Moreover, transcription factors and histone modifications mark the sns5 chromatin at the integration site (4), suggesting that sns5 displays mechanisms of action common to other well characterized insulators.
Here we show that sns5 increases the likelihood and the expression of a \uf062-globin/lentiviral vector integrated as a single copy in both murine cell clones and in a mouse model of \uf062-thalassemia.
It has been proposed that two copies of insulators may direct the formation of a chromatin loop by interaction among protein complexes assembled on their sequences (5). Intriguingly, by using the 3C technology, we found that sns5-flanked vectors integrated at a single copy in the resident genome are specifically organized into an independent chromatin structure.
Our findings highlight that sns5 could be a promising tool for improving the performance of vectors in the field of gene therapy.
1. Gaszner and Felsenfeld (2006). Nat Rev Genet 7:703-13
2. Palla et al (1997). PNAS USA 94:2272-7
3. Cavalieri et al (2009). Nucleic Acids Res 37:7407-15
4. D'Apolito et al (2009). Mol Ther 17:1434-41
5. Wallace and Felsenfeld (2007). Curr Op Genet Dev 17:400-
Turbulence in Rivers
The study of turbulence has always been a challenge for scientists working on geophysical flows. Turbulent flows are common in nature and have an important role in geophysical disciplines such as river morphology, landscape modeling, atmospheric dynamics and ocean currents. At present, new measurement and observation techniques suitable for fieldwork can be combined with laboratory and theoretical work to advance the understanding of river processes. Nevertheless, despite more than a century of attempts to correctly formalize turbulent flows, much still remains to be done by researchers and engineers working in hydraulics and fluid mechanics. In this contribution we introduce a general framework for the analysis of river turbulence. We revisit some findings and theoretical frameworks and provide a critical analysis of where the study of turbulence is important and how to include detailed information of this in the analysis of fluvial processes. We also provide a perspective of some general aspects that are essential for researchers/ practitioners addressing the subject for the first time. Furthermore, we show some results of interest to scientists and engineers working on river flows
Analytical solution of kinematic wave time of concentration for overland flow under green-ampt infiltration
In this paper the well-known kinematic wave equation for computing the time of concentration for impervious surfaces has been extended to the case of pervious hillslopes, accounting for infiltration. An analytical solution for the time of concentration for overland flow on a rectangular plane surface is derived using the kinematic wave equation under the Green-Ampt infiltration. The relative time of concentration is defined as the ratio between the time of concentration of an infiltrating plane and the soil sorptivity time scale, depending on the normalized rainfall intensity and a parameter synthesizing the soil and hillslope characteristics. It is shown that for a more complex case (corresponding to the second domain of solution domain), the time of concentration can also be estimated by two simplified approximate procedures. An error analysis for the time of concentration computed for constant and time-varying infiltration is carried out. Finally, for a hillslope under the Green-Ampt infiltration, the time of concentration obtained by the kinematic wave equation is compared with that computed by a nonlinear storage model. An application of the proposed method for two different soils is shown and discussed
Determining Probability Distribution of Hillslope Peak Discharge by Using an Analytical Solution of Kinematic Wave Time of Concentration.
Hillslope hydrology is fundamental for understanding the flood phenomenon and for evaluating the time of
concentration. The latter is a key variable for predicting peak discharge at the basin outlet and for designing urban
infrastructure facilities. There have been a multitude of studies on the hydrologic response at the hillslope scale,
and the time of concentration has been derived for different approaches. One approach for deriving hillslope
response utilizes, in a distributed form, the differential equations of unsteady overland flow, specifically developed
at the hydrodynamic scale, in order to account for the spatial heterogeneity of soil characteristics, topography,
roughness and vegetation cover on the hillslope. Therefore, this approach seemingly mimics the complete
hydraulics of flow. However, the very complex patterns generated by spatial heterogeneity can cause considerable
error in the prediction even by very sophisticated models. Another approach that directly operates at the hillslope
scale is by averaging over the hillslope the soil hydraulics, the topography, and the roughness characteristics. A
physically-based lumped model of hillslope response was first proposed by Horton (1938), under the assumption
that the flow regime is intermediate between laminar and turbulent regimes (transitional flow regime), by applying
the mass conservation equation to the hillslope as a whole and by using the kinematic wave assumption for
the friction slope (Singh, 1976, 1996). Robinson et al. (1995) and Robinson and Sivapalan (1996) generalized
Horton\u2019s approach, suggesting an approximate solution of the overland flow equation that is valid for all flow
regimes. Agnese et al. (2001) derived an analytical solution of a nonlinear storage model of hillslope response that
is valid for all flow regimes, and the associated time of concentration.
Recently, the well-known kinematic wave equation for computing the time of concentration for impervious
surfaces has been extended to the case of pervious hillslopes, accounting for infiltration. In particular, an analytical
solution for the time of concentration for overland flow on a rectangular plane surface was derived using the
kinematic wave equation under the Green-Ampt infiltration (Baiamonte and Singh, 2015). The objective of
this work is to apply the latter solution to determine the probability distribution of hillslope peak discharge by
combining it with the familiar rainfall duration-intensity-frequency approach
Overland Flow Times of Concentration for Hillslopes of Complex Topography
The time of concentration is an important parameter for predicting peak discharge at the basin outlet and for designing urban infrastructure facilities. In studying the hillslope response, employing hydraulic equations of flow, the shape of the hillslope geometry has often been assumed as rectangular and planar. However, natural hillslopes have complex topographies whose shapes are characterized by irregularly spaced contour lines. Recently, kinematic wave time of concentration has been derived for rectangular and curved parallel hillslopes. This paper extends this work to hillslopes of complex planform geometry, considering the degree of divergence or convergence of the hillslope. The extended formulation consists of only one equation that is valid for both divergent/convergent surfaces and for concave/convex hillslope profile, and is compared with the formulations for plane convergent and plane divergent surfaces previously introduced. Results are compared with those already available in the literature, which were obtained by using the nonlinear storage model applied to the same complex hillslopes
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