Symposium review: Decomposing efficiency of milk production and maximizing profit

The dairy industry has focused on maximizing milk yield, as it is believed that this maximizes profit mainly through dilution of maintenance costs. Efficiency of milk production has received, until recently, considerably less attention. The most common method to determine biological efficiency of milk production is feed efficiency (FE), which is defined as the amount of milk produced relative to the amount of nutrients consumed. Economic efficiency is best measured as income over feed cost or gross margin obtained from feed investments. Feed efficiency is affected by a myriad of factors, but overall they could be clustered as follows: (1) physiological status of the cow (e.g., age, state of lactation, health, level of production, environmental conditions), (2) digestive function (e.g., feeding behavior, passage rate, rumen fermentation, rumen and hindgut microbiome), (3) metabolic partitioning (e.g., homeorhesis, insulin sensitivity, hormonal profile), (4) genetics (ultimately dictating the 2 previous aspects), and (5) nutrition (e.g., ration formulation, nutrient balance). Over the years, energy requirements for maintenance seem to have progressively increased, but efficiency of overall nutrient use for milk production has also increased due to dilution of nutrient requirements for maintenance. However, empirical evidence from the literature suggests that marginal increases in milk require progressively greater marginal increases in nutrient supply. Thus, the dilution of maintenance requirements associated with increases in production is partially overcome by a progressive diminishing marginal biological response to incremental energy and protein supplies. Because FE follows the law of diminishing returns, and because marginal feed costs increase progressively with milk production, profits associated with improving milk yield might, in some cases, be considerably lower than expected.info:eu-repo/semantics/publishedVersio

Feeding pasteurized waste milk to preweaned dairy calves changes fecal and upper respiratory tract microbiota

In the present study bacterial communities from both, the gastrointestinal and respiratory tract of pre-weaned dairy calves fed two different milk-feeding programs were characterized using 16S rRNA gene sequencing. Twenty female Holstein calves (38.8 ± 1.40 kg of BW) were fed pasteurized waste milk (pWM) containing residues of various antimicrobials. Twenty additional calves (38.1 ± 1.19 kg of BW) were fed milk replacer (MR) with similar nutrient composition (27.5% crude protein, 32.1% fat) compared to waste milk (28.6% crude protein, 30.0% fat) from day 1 to weaning at day 49 of study. Fecal samples and nasal swabs were collected on day 42 only from calves that were not treated with therapeutic antibiotics throughout the study, which were 8 MR and 10 pWM calves. To assess the impact of the two feeding regimes on the fecal and nasal microbiota, α and β-diversity measures were calculated, and the relative abundance of operational taxonomic units (OTUs) at different taxonomic levels was determined for each sample. In general, Chao1, PD Whole Tree, and Shannon diversity indices were similar for the fecal and nasal bacterial communities of calves regardless of the feeding regime. However, principal coordinate analysis based on unweighted Unifrac distances indicated differences in the structure of bacterial communities of calves fed milk replacer compared with those from calves fed pasteurized waste milk. The relative abundance of the Streptococcaceae family and the genus Histophilus was greater (P < 0.05) in the nasal microbiota of calves fed milk replacer than in those fed pasteurized waste milk. However, the genus Prevotella tended (P = 0.06) to be more relatively abundant in the respiratory tract of calves fed pasteurized waste milk than in those fed milk replacer. Differences in relative abundances of bacterial taxa in gut microbiota were only observed at the phylum level, suggesting that antimicrobial residues present in waste milk have a non-specific influence at a lower taxonomical level.info:eu-repo/semantics/publishedVersio

Using compositional mixed-effects models to evaluate responses to amino acid supplementation in milk replacers for calves

The consequences of supplementing Lys, Met, and Thr in milk replacers (MR) for calves have been widely studied, but scarce information exists about potential roles of other AA (whether essential or not). The effects on growth performance of supplementation of 4 different AA combinations in a mixed ration (25.4% crude protein and 20.3% fat) based on skim milk powder and whey protein concentrate were evaluated in 76 Holstein male calves (3 ± 1.7 d old). The 4 MR were as follows: CTRL with no AA supplementation; PG, supplying additional 0.3% Pro and 0.1% Gly; FY, supplying additional 0.2% Phe and 0.2% Tyr; and KMT, providing additional 0.62% Lys, 0.22% Met, and 0.61% Thr. All calves were fed the same milk allowance program and were weaned at 56 d of study. Concentrate intake was limited to minimize interference of potential differences in solid feed intake among treatments. Animals were weighed weekly, intakes recorded daily, and blood samples obtained at 2, 5, and 7 wk of study to determine serum urea and plasma AA concentrations. Plasma AA concentrations were explored using compositional data analysis, and their isometric log-ratio transformations were used to analyze their potential influence on ADG and serum urea concentration using a linear mixed-effects model. We detected no differences in calf performance and feed intake. Plasma relative concentration of the AA supplemented in the KMT and PG treatments increased in their respective treatments, and, in PG calves, a slight increase in the proportion of plasma Gly, Glu, and branched-chain AA was also observed. The proportions of plasma branched-chain AA, His, and Gln increased, and those of Thr, Arg, Lys, and Glu decreased with calves' age. A specific log-contrast balance formed by Arg, Thr, and Lys was found to be the main driver for lowering serum urea concentrations and increasing calf growth. The use of compositional mixed-effects models identified a cluster formed by the combination of Arg, Thr, and Lys, as a potential AA to optimize calf growth.info:eu-repo/semantics/publishedVersio

Effectiveness of precision feeding in reducing N excretion in dairy cattle

Two periods enrolling 56 (milk yield 36.2 ± 7.91 kg/d) and 58 (milk yield 32.4 ± 4.99 kg/d) grouped-fed dairy cows were performed to evaluate nutrient adjustment and N emissions of a precision feeding strategy. Pens blocked by parity were randomly assigned to a conventional (CONV) or to a precision feeding scheme (PREC) for a 21-d period. The CONV group was offered a total mixed ration (TMR, 6.82 and 6.65 MJ of NEl/kg of DM, in period 1 and 2, respectively, and 165 g of CP/kg of DM in both periods; whereas PREC cows were fed a partial mixed ration (PMR, 6.65 and 6.40 MJ of NEl/kg of DM, 135 and 137 g of CP/kg of DM, in period 1 and 2, respectively) and a concentrate feed supplemented twice daily in the milking parlour, which contained different quantities of soybean meal, corn meal, and wheat middling’s according to estimated nutritional needs of each cow above those supplied by the consumption of PMR. Individual daily nutritional needs and nutrients consumed from the PMR were calculated using a 10-d rolling average of performance data (milk yield and concentration of its components, and BW daily recorded in both periods). A N balance using urine and fecal spot sampling during the last 3 d of the study was performed in period 1, and stored manure gaseous emissions (ammonia, methane, nitrous oxide, and carbon dioxide) were measured for 2 wk in period 2. After 2 wk of adaptation to the diet, 82 cows homogeneously distributed in 4 DIM categories: early DIM ( 220) were used to assess how energy and protein requirements were adjusted using both feeding system. Dairy cows in both feeding systems were energetically overfed, and CONV cows tended to be more CP overfed in mid-late and late DIM cows than PREC fed cows. Total daily N urine excretion, and milk N urea concentration were greater in CONV than in PREC cows. There were no differences in ammonia and nitrous oxide emissions from the manure storage between PREC and CONV cows; however, methane and carbon dioxide emissions from manure increased by 55% and 15%, respectively in PREC fed cows. Precision feeding system based on preceding average daily milk yield and composition can reduce N excretion without affecting short-term milking performance but increasing C gaseous emissions from manure.This research was carried out in the framework of the project CIRCULAR AGRONOMICS (nº contract agreement 773649). IRTA thanks the support of the CERCA Program and the Consolidated Research Groups of Sustainability in Biosystems (ref. 2021 SGR 01568) and Sustainable Animal Husbandry (ref. 2021 SGR 01552), both from the Generalitat de Catalunya. Lluis Morey thanks Universitat Politècnica de Catalunya and the financial support of AGAUR, of the Generalitat de Catalunya (grant reference number 2019FI_B 00694)info:eu-repo/semantics/publishedVersio

Using compositional mixed-effects models to evaluate responses to amino acid supplementation in milk replacers for calves

The consequences of supplementing Lys, Met, and Thr in milk replacers (MR) for calves have been widely studied, but scarce information exists about potential roles of other AA (whether essential or not). The effects on growth performance of supplementation of 4 different AA combinations in a mixed ration (25.4% crude protein and 20.3% fat) based on skim milk powder and whey protein concentrate were evaluated in 76 Holstein male calves (3 ± 1.7 d old). The 4 MR were as follows: CTRL with no AA supplementation; PG, supplying additional 0.3% Pro and 0.1% Gly; FY, supplying additional 0.2% Phe and 0.2% Tyr; and KMT, providing additional 0.62% Lys, 0.22% Met, and 0.61% Thr. All calves were fed the same milk allowance program and were weaned at 56 d of study. Concentrate intake was limited to minimize interference of potential differences in solid feed intake among treatments. Animals were weighed weekly, intakes recorded daily, and blood samples obtained at 2, 5, and 7 wk of study to determine serum urea and plasma AA concentrations. Plasma AA concentrations were explored using compositional data analysis, and their isometric log-ratio transformations were used to analyze their potential influence on ADG and serum urea concentration using a linear mixed-effects model. We detected no differences in calf performance and feed intake. Plasma relative concentration of the AA supplemented in the KMT and PG treatments increased in their respective treatments, and, in PG calves, a slight increase in the proportion of plasma Gly, Glu, and branched-chain AA was also observed. The proportions of plasma branched-chain AA, His, and Gln increased, and those of Thr, Arg, Lys, and Glu decreased with calves' age. A specific log-contrast balance formed by Arg, Thr, and Lys was found to be the main driver for lowering serum urea concentrations and increasing calf growth. The use of compositional mixed-effects models identified a cluster formed by the combination of Arg, Thr, and Lys, as a potential AA to optimize calf growth.Peer ReviewedPostprint (published version

Exploring the use of tertiary reclaimed water in dairy cattle production

The objective of this study was to explore through both in vitro and in vivo experiments the use of reclaimed urban wastewater in dairy cattle production systems with the aim of improving water efficiency and sustainability. Firstly, the use of different tertiary treatments (ultrafiltration (UF), ultraviolet disinfection (UV), chlorination process, and their combination) to improve the quality of an urban secondary effluent was studied in intestinal primary cell cultures evaluating the expression of genes related to apoptosis, cell damage, and inflammation. The results revealed that secondary treated wastewater and waters that were treated with a chlorination process (even tap water) caused an increase in apoptosis, intestinal primary cell damage, and inflammation. The in vivo experiment evaluated the short-term effects on health and performance of using UF- and UV-treated secondary effluent compared with the use of tap water for drinking and preparing milk replacer in young calves from 5 to 47 days of age. Calves previously fed with UF + UV treated secondary effluent clearly preferred tap water when they were exposed to a double water choice at the end of the study. This reduction of the palatability and acceptability was probably due to a greater level of water salinity of the treated reclaimed water (570 vs 1437 ± 76.5 μS/cm of conductivity for tap water and UF-UV treated secondary effluent, respectively), which potentially entailed a reduction of calf concentrate intake (466 vs 351 ± 32.2 g/d for calves fed with tap water and UF-UV treated water, respectively). The use of reclaimed water did not pose an acute risk to animal health. It is concluded that improvements on the tertiary treatment to reduce water salinity should be considered when using reclaimed water for drinking purposes in livestock production systems. This study is a first approach to a more sustainable and efficient use of water in animal husbandry for countries with water scarcity. However, more studies are required before its implementation to further study long-term effects and the presence of new-contaminants not defined in the current legislation.info:eu-repo/semantics/acceptedVersio

Serpentinization-Driven H2 Production From Continental Break-Up to Mid-Ocean Ridge Spreading: Unexpected High Rates at the West Iberia Margin

Molecular hydrogen (H2) released during serpentinization of mantle rocks is one of the main fuels for chemosynthetic life. Processes of H2 production at slow-spreading mid-ocean ridges (MORs) have received much attention in the past. Less well understood is serpentinization at passive continental margins where different rock types are involved (lherzolite instead of harzburgite/dunite at MORs) and the alteration temperatures tend to be lower (&lt;200°C vs. &gt;200°C). To help closing this knowledge gap we investigated drill core samples from the West Iberia margin. Lherzolitic compositions and spinel geochemistry indicate that the exhumed peridotites resemble sub-continental lithospheric mantle. The rocks are strongly serpentinized, mainly consist of serpentine with little magnetite, and are generally brucite-free. Serpentine can be uncommonly Fe-rich, with XMg = Mg/(Mg + Fe) &lt; 0.8, and shows distinct compositional trends toward a cronstedtite endmember. Bulk rock and silicate fraction Fe(III)/∑Fe ratios are 0.6–0.92 and 0.58–0.8, respectively; our data show that 2/3 of the ferric Fe is accounted for by Fe(III)-serpentine. Mass balance and thermodynamic calculations suggest that the sample’s initial serpentinization produced ∼120 to &gt;300 mmol H2 per kg rock. The cold, late-stage weathering of the serpentinites at the seafloor caused additional H2 formation. These results suggest that the H2 generation potential evolves during the transition from continental break-up to ultraslow and, eventually, slow MOR spreading. Metamorphic phase assemblages systematically vary between these settings, which has consequences for H2 yields during serpentinization. At magma-poor rifted margins and ultraslow-spreading MORs, serpentine hosts most Fe(III). Hydrogen yields of 120 to &gt;300 mmol and 50–150 mmol H2 per kg rock, respectively, may be expected at temperatures of &lt;200°C. At slow-spreading MORs, in contrast, serpentinization may produce 200–350 mmol H2, most of which is related to magnetite formation at &gt;200°C. Since, in comparison to slow-spreading MORs, geothermal gradients at magma-poor margins and ultraslow-spreading MORs are lower, larger volumes of low-temperature serpentinite should form in these settings. Serpentinization of lherzolitic rocks at magma-poor margins should produce particularly high amounts of H2 under conditions within the habitable zone. Magma-poor margins may hence be more relevant environments for hydrogenotrophic microbial life than previously thought