2 research outputs found
Production of monoclonal and polyclonal antibodies against a Nigerian isolate of banana streak virus
Banana streak virus is serologically and genomically heterogenous
worldwide and there has been the need to produce antibodies that can
detect all known serotypes of this virus. Antibody production requires
purified virus, since BSV titre is low in Musa tissues, there was the
need for an efficient method of purifying the virus. We are reporting
the first production of two monoclonal antibodies, BSV 3F9/1 and BSV
3D4/2, against an isolate of BSV. Culture fluids of BSV 3F9/1 and BSV
3D4/2 had antibody titres of 1:204,000 and 1:6400, by ELISA,
respectively. The two monoclonal antibodies detected all isolates of
BSV that were detected by the homologous mouse polyclonal antibodies.
Caesium gradient centrifugation reduced yield of BSV during
purification. We described a method of purification, which excluded the
caesium gradient step and yet increased BSV yield by about 15-fold. The
virus preparation obtained by this new method was used to produce
BSV-specific mouse and rabbit polyclonal antibodies. These BSV
monoclonal antibodies together with the polyclonal antibodies were used
for the detection of BSV