34 research outputs found

    Qualitative Real-Time PCR SYBR Green Detection of Petri Disease Fungi

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    Real-time PCR provides a fast, reliable, and cost-effective method for detecting the presence or absence of Petri disease fungi in grapevines. The primer pairs, Pmo1f + Pmo2r, and Pac1f + Pac2r, were designed for species and genus-specific amplification of Phaeomoniella chlamydospora and Phaeoacremonium spp. respectively, using realtime PCR with SYBR® Green. The primers were specific and showed no primer-primer dimers until after 35 cycles. Pa. chlamydospora was detected in roots, shoots, and young trunks of drill-inoculated vines. Phaeoacremonium was detected in trunk cross-sections of naturally infected vines from which Phaeoacremonium aleophilum had been isolated. The protocol presented here can be adapted to provide a reliable detection system for research and industry

    SU(2) Calorons and Magnetic Monopoles

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    We investigate the self-dual Yang-Mills gauge configurations on R3Ă—S1R^3\times S^1 when the gauge symmetry SU(2) is broken to U(1) by the Wilson loop. We construct the explicit field configuration for a single instanton by the Nahm method and show that an instanton is composed of two self-dual monopoles of opposite magnetic charge. We normalize the moduli space metric of an instanton and study various limits of the field configuration and its moduli space metric.Comment: 17 pages, RevTex, 1 Figur

    The Physics of the B Factories

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    Absorption Spectra in the Vacuum Ultraviolet and the Ionization Potentials of Naphthalene and Naphthalene-d8 Molecules

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    The vapour absorption spectra of naphthalene and naphthalene-d8 were obtained photographically and photoelectrically from 50000 to 70000 cm-1. A series of Rydberg transitions and possibly two Π-Π systems are observed. Each of the five Rydberg series converges to an ionization potential of 65620 ± 40 cm-l, i.e. 8.136 ± 0.005 eV (C10H8), and 65640 ± 60 cm-l, i.e. 8.138 ±0.008 eV (C10D8). The quantum defects of the series are (0.94, 0.88, 0.82, 0.67, 0.53) ± 0.01. The significance of these results is discussed

    Utilizing visual attention for cross-modal coreference interpretation

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    Abstract. In this paper, we describe an exploratory study to develop a model of visual attention that could aid automatic interpretation of exophors in situated dialog. The model is intended to support the reference resolution needs of embodied conversational agents, such as graphical avatars and robotic collaborators. The model tracks the attentional state of one dialog participant as it is represented by his visual input stream, taking into account the recency, exposure time, and visual distinctness of each viewed item. The model correctly predicts the correct referent of 52 % of referring expressions produced by speakers in human-human dialog while they were collaborating on a task in a virtual world. This accuracy is comparable with reference resolution based on calculating linguistic salience for the same data.
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