8 research outputs found
Quantitation of fluorescence energy transfer between cell surface proteins via fluorescence donor photobleaching kinetics
No full textRotational dynamics of type I Fc epsilon receptors on individually-selected rat mast cells studied by polarized fluorescence depletion
No full textKinetics of binding of 2,4-dinitrophenyl and 2,4,6-trinitrophenyl haptens to homologous and heterologous rabbit antibodies
No full textKinetic evidence for a conformational change by an anti-hapten antibody upon binding multivalent hapten
No full textImaging molecular interactions in cells by dynamic and static fluorescence anisotropy (rFLIM and emFRET)\ud
Get PDFWe report the implementation and exploitation of fluorescence polarization measurements, in the form of anisotropy fluorescence lifetime imaging microscopy (rFLIM) and energy migration Förster resonance energy transfer (emFRET) modalities, for wide-field, confocal laser-scanning microscopy and flow cytometry of cells. These methods permit the assessment of rotational motion, association and proximity of cellular proteins in vivo. They are particularly applicable to probes generated by fusions of visible fluorescence proteins, as exemplified by studies of the erbB receptor tyrosine kinases involved in growth-factor-mediated signal transduction.\ud
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